Rise and decay kinetics of photosensitized singlet oxygen luminescence in water. Measurements with nanosecond time-correlated single photon counting technique

Егоров, С. А.; Kamalov, Valey; Koroteev, N. I.; Krasnovsky, А.А.; Toleutaev, B. N.; Zinukov, S. V.

doi:10.1016/0009-2614(89)85161-9

Cited by 196 publications

(114 citation statements)

References 9 publications

Supporting

Mentioning

108

Contrasting

Order By: Relevance

“…This proves that the process of excitation energy transfer from photosensitizers to oxygen follows bimolecular mechanism even in the range of much lower oxygen concentrations than that was published in Ref. [7]. The value of the respective bimolecular quenching constant was determined as (1.570.1) Â 10 9 l/mol s, which is in a perfect agreement with our previously published value of (1.770.8) Â 10 9 l/mol s [1] and 1.8 Â 10 9 l/ mol s obtained by Mosinger et al [8], this time with significantly higher precision.…”

Section: Resultsmentioning

confidence: 57%

Singlet oxygen quenching by oxygen in tetraphenyl-porphyrin solutions

Dědic¹,

Kořı́nek²,

Molnar³

et al. 2006

Journal of Luminescence

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 57%

Singlet oxygen quenching by oxygen in tetraphenyl-porphyrin solutions

Dědic¹,

Kořı́nek²,

Molnar³

et al. 2006

Journal of Luminescence

View full text Add to dashboard Cite

“…µs 19 whereas it is ~ 3.5 µs in H 2 O, 20 and this difference is manifested in the quantum efficiency of singlet oxygen phosphorescence. 2 16 In most of our previous singlet oxygen work in cells, we used a hydrophilic cationic porphyrin as a photosensitizer: 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H, 23H-porphine (TMPyP).…”

Section: Introductionmentioning

confidence: 95%

Singlet Oxygen in a Cell: Spatially Dependent Lifetimes and Quenching Rate Constants

2008

View full text Add to dashboard Cite

Singlet molecular oxygen, O 2 (a 1 ∆ g ), can be created in a single cell from ground state oxygen, O 2 (X 3 Σ g -), upon focused laser irradiation of an intracellular sensitizer. This cytotoxic species can subsequently be detected by its 1270 nm phosphorescence (a 1 ∆ g → X 3 Σ g -) with subcellular spatial resolution. The singlet oxygen lifetime determines its diffusion distance and, hence, the intracellular volume element in which singlet-oxygen-initiated perturbation of the cell occurs. In this study, the time-resolved phosphorescence of singlet oxygen produced by the sensitizers chlorin (Chl) and 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H,23H-porphine (TMPyP) was monitored. These molecules localize in different domains of a living cell. The data indicate that (i) the singlet oxygen lifetime and (ii) the rate constant for singlet oxygen quenching by added NaN 3 depend on whether Chl or TMPyP was the photosensitizer.These observations likely reflect differences in the chemical and physical constituency of a given subcellular domain (e.g., spatially-dependent oxygen and NaN 3 diffusion coefficients) and, as such, are evidence that singlet oxygen responds to the inherent heterogeneity of a cell.Thus, despite a relatively long intracellular lifetime, singlet oxygen does not diffuse a great distance from its site of production. This is a consequence of an apparent intracellular viscosity that is comparatively large.3

show abstract

“…Summary of measurements.Note: Literature values of ⌬ in acetone, methanol, and water are 51, 9.5, and 4.2 s, respectively [13][14][15][17][18][19]. …”

mentioning

confidence: 99%

Pulsed diode laser-based monitor for singlet molecular oxygen

et al. 2008

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in the metastable singlet delta state O2(1Delta) are believed to be the species that destroys cancerous cells during PDT. Monitoring singlet oxygen produced by PDT may lead to more precise and effective PDT treatments. Our approach uses a pulsed diode laser-based monitor with optical fibers and a fast data acquisition system to monitor singlet oxygen during PDT. We present results of in vitro singlet oxygen detection in solutions and in a rat prostate cancer cell line as well as PDT mechanism modeling.

show abstract

Rise and decay kinetics of photosensitized singlet oxygen luminescence in water. Measurements with nanosecond time-correlated single photon counting technique

Cited by 196 publications

References 9 publications

Singlet oxygen quenching by oxygen in tetraphenyl-porphyrin solutions

Singlet oxygen quenching by oxygen in tetraphenyl-porphyrin solutions

Singlet Oxygen in a Cell: Spatially Dependent Lifetimes and Quenching Rate Constants

Pulsed diode laser-based monitor for singlet molecular oxygen

Contact Info

Product

Resources

About