RISC assembly: Coordination between small RNAs and Argonaute proteins

Kobayashi, Hotaka; Tomari, Yukihide

doi:10.1016/j.bbagrm.2015.08.007

Cited by 251 publications

(207 citation statements)

References 198 publications

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“…In addition, by RIP assays, we also found that TUG1 and miR-299 were in the same RISC, suggesting that TUG1 and miR-299 were present in one RISC, consistent with our bioinformatic analysis and luciferase assays, which could be supported by other studies. 43 HOTAIR and miR-331-3p both bind with Ago2 in the same RNA-induced silencing complex. 18 GAS5 was a potential tumor suppressor and executed the anti-cancer function in part through the repression of miR-21 in the same RISC complex.…”

Section: Discussionmentioning

confidence: 98%

Long non-coding RNA taurine upregulated 1 enhances tumor-induced angiogenesis through inhibiting microRNA-299 in human glioblastoma

et al. 2016

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Angiogenesis is one of the critical biological elements affecting the development and progression of cancer. Long non-coding RNAs (lncRNAs) are important regulators and aberrantly expressed in various types of human cancer. Our previous studies indicated that lncRNA taurine upregulated 1 (TUG1) implicated in the regulation of blood-tumor barrier permeability; however, its role in glioblastoma angiogenesis still unclear. Here we demonstrated that TUG1 was up-expressed in human glioblastoma tissues and glioblastoma cell lines. Knockdown of TUG1 remarkably suppressed tumor-induced endothelial cell proliferation, migration and tube formation as well as reducing spheroid-based angiogenesis ability in vitro, which are the critical steps for tumor angiogenesis. Besides, knockdown of TUG1 significantly increased the expression of mircroRNA-299 (miR-299), which was down-expressed in glioblastoma tissues and glioblastoma cell lines. Bioinformatics analysis and luciferase reporter assay revealed that TUG1 influenced tumor angiogenesis via directly binding to the miR-299 and there was a reciprocal repression between TUG1 and miR-299 in the same RNA-induced silencing complex. Moreover, knockdown of TUG1 reduced the expression of vascular endothelial growth factor A (VEGFA), which was defined as a functional downstream target of miR-299. In addition, knockdown of TUG1, shown in the in vivo studies, has effects on suppressing tumor growth, reducing tumor microvessel density and decreasing the VEGFA expression by upregulating miR-299 in xenograft glioblastoma model. Overall, the results demonstrated that TUG1 enhances tumor-induced angiogenesis and VEGF expression through inhibiting miR-299. Also, the inhibition of TUG1 could provide a novel therapeutic target for glioblastoma treatment.

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Section: Discussionmentioning

confidence: 98%

Long non-coding RNA taurine upregulated 1 enhances tumor-induced angiogenesis through inhibiting microRNA-299 in human glioblastoma

et al. 2016

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“…In the cytoplasm, the RNase III enzyme Dicer processes the pre-miRNA to an 18-23 nucleotides (nt) long double-stranded (ds) intermediate. One strand is selected and gives rise to the mature miRNA, while the other strand is degraded (Dueck and Meister, 2014;Kobayashi and Tomari, 2015). The mature miRNA is incorporated into the RNA-induced silencing complex (RISC), where it binds to an Argonaute protein of the Ago family (Meister, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Lupus Autoantigen La Prevents Mis-channeling of tRNA Fragments into the Human MicroRNA Pathway

et al. 2016

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The Lupus autoantigen La is an RNA-binding protein that stabilizes RNA polymerase III (Pol III) transcripts and supports RNA folding and has in addition been implicated in the mammalian microRNA (miRNA) pathway. Here, we have analyzed effects of La depletion on Argonaute (Ago)-bound small RNAs in human cells. We find that in the absence of La, distinct tRNA fragments are loaded into Ago proteins. Thus, La functions as gatekeeper ensuring correct tRNA maturation and protecting the miRNA pathway from potentially functional tRNA fragments. However, one specific isoleucin pre-tRNA produces both a functional tRNA and a miRNA even when La is present. We demonstrate that the fully complementary 5' leader and 3' trailer of the pre-tRNA-Ile form a double-stranded RNA molecule that has low affinity to La. Instead, Exportin-5 (Xpo5) recognizes it as miRNA precursor and transports it into the cytoplasm for Dicer processing and Ago loading.

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“…Processing of double stranded RNA (dsRNA) into small RNA (sRNA) duplexes of 21–24 nucleotides is governed by RNase III-like Dicer-like (DCL) enzymes (Bologna and Voinnet, 2014). The sRNAs are incorporated into the Argonaute (AGO) protein complex to direct slicing and degradation of RNA molecules with sequence homology to the AGO-bound sRNAs (Carbonell and Carrington, 2015, Kobayashi and Tomari, 2016, Meister, 2013). The Arabidopsi s thaliana genome encodes four DCL and ten AGO families, many of which participate in antiviral defense (Carbonell and Carrington, 2015, Garcia-Ruiz et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Differential response of cassava genotypes to infection by cassava mosaic geminiviruses

et al. 2017

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HighlightsCassava genotypes respond differently to infection by cassava mosaic geminiviruses.Cassava mosaic disease resistant loci prompt recovery from systemic infection.CMD symptoms are directly correlated with contents of viral DNA and virus specific small RNAs.CMD infected plants abundantly accumulate 21–24 nt of virus specific small RNAs.VsRNAs heterogeneously map the entire virus genome in both polarities.

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RISC assembly: Coordination between small RNAs and Argonaute proteins

Cited by 251 publications

References 198 publications

Long non-coding RNA taurine upregulated 1 enhances tumor-induced angiogenesis through inhibiting microRNA-299 in human glioblastoma

Long non-coding RNA taurine upregulated 1 enhances tumor-induced angiogenesis through inhibiting microRNA-299 in human glioblastoma

The Lupus Autoantigen La Prevents Mis-channeling of tRNA Fragments into the Human MicroRNA Pathway

Differential response of cassava genotypes to infection by cassava mosaic geminiviruses

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