RIPK1 binds to vitamin D receptor and decreases vitamin D-induced growth suppression

Quarni, Waise; Lungchukiet, Panida; Tse, Anfernee; Wang, Pei; Sun, Yinyan; Kasiappan, Ravi; Wu, Jheng-Yu; Zhang, Xiaohong; Bai, Wenlong

doi:10.1016/j.jsbmb.2017.01.024

Cited by 4 publications

(4 citation statements)

References 46 publications

(49 reference statements)

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“…Venous blood Samples were taken for the es ma on of 25(OH) Vitamin D, Calcium and Phosphate. Sta s cal analysis was done using SPSS so ware version 16.…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Status Among Children aged 2 months to 13 years-a Hospital Based Study

Rimal

Walavalkar

2018

Birat J. Health Sci.

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Introduction: Vitamin D is an important micronutrient having crucial role in calcium and phosphate homeostasis but there is emerging evidence to suggest its role in prevention of infectious, inflammatory and neoplastic diseases. The vitamin D insufficiency/deficiency is a widely recognized problem among children in developed as well as developing countries.Objective: To find out the prevalence of vitamin D deficiency/insufficiency among children aged 2 months to 13 yrs and its correlation with serum calcium, phosphate and demographic factors.Methodology: Prospective, cross sectional, hospital based study conducted at Birat Medical College Teaching Hospital for a period of one year among children presented to pediatric OPD in whom pediatrician had a suspicion of Vitamin D Insufficiency /Deficiency. Venous blood Samples were taken for the estimation of 25(OH) Vitamin D, Calcium and Phosphate. Statistical analysis was done using SPSS software version 16. Results Among 174 children aged 2 months to 13 years, the prevalence of vitamin D insufficiency at the cutoff of <30ng/ml was 82.75% where as no cases of vitamin D deficiency was noted at cut off level of < 10 ng/ml. Children residing in urban area, samples taken in the winter season had higher prevalence of vitamin D insufficiency (p value <0.05). Serum calcium and phosphate level were found positively correlated with Vitamin D level (p<.05). However the relationship was very strong between Vitamin D and Calcium (r=0.402, p-value <0.001) and weak positive correlation was noted with phosphate(r=0.155, p value <0.05).Conclusion: There is very high prevalence of Vitamin D insufficiency among children. The vitamin D insufficiency was significantly higher in winter season as compared to summer. Children living in the urban area are more prone to Vitamin D insufficiency. Moderately strong positive correlation was observed between Vitamin D level and calcium but weak correlation existed with phosphate. BJHS 2018;3(1)5 : 381-384

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“…Venous blood Samples were taken for the es ma on of 25(OH) Vitamin D, Calcium and Phosphate. Sta s cal analysis was done using SPSS so ware version 16.…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Status Among Children aged 2 months to 13 years-a Hospital Based Study

Rimal

Walavalkar

2018

Birat J. Health Sci.

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“…Consistent with the observation, RIPK1 was found that formed a complex with VDR and retained VDR in cytoplasm in MEFs and ovarian cancer cells. The formed complex not only blocked transcriptional activity of VDR, but also disturbed the necrosome formation (225).…”

Section: Vitamin D and Regulated Necrosismentioning

confidence: 99%

Crosstalk between regulated necrosis and micronutrition, bridged by reactive oxygen species

Zhang

Liu²,

Dai³

et al. 2022

Front. Nutr.

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The discovery of regulated necrosis revitalizes the understanding of necrosis from a passive and accidental cell death to a highly coordinated and genetically regulated cell death routine. Since the emergence of RIPK1 (receptor-interacting protein kinase 1)-RIPK3-MLKL (mixed lineage kinase domain-like) axis-mediated necroptosis, various other forms of regulated necrosis, including ferroptosis and pyroptosis, have been described, which enrich the understanding of pathophysiological nature of diseases and provide novel therapeutics. Micronutrients, vitamins, and minerals, position centrally in metabolism, which are required to maintain cellular homeostasis and functions. A steady supply of micronutrients benefits health, whereas either deficiency or excessive amounts of micronutrients are considered harmful and clinically associated with certain diseases, such as cardiovascular disease and neurodegenerative disease. Recent advance reveals that micronutrients are actively involved in the signaling pathways of regulated necrosis. For example, iron-mediated oxidative stress leads to lipid peroxidation, which triggers ferroptotic cell death in cancer cells. In this review, we illustrate the crosstalk between micronutrients and regulated necrosis, and unravel the important roles of micronutrients in the process of regulated necrosis. Meanwhile, we analyze the perspective mechanism of each micronutrient in regulated necrosis, with a particular focus on reactive oxygen species (ROS).

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“…This might be achieved through the shift of VDR from the nucleus into the cytosol or through the retention of VDR in the cytosol. 35 Despite the overall reduction in VDR expression in colitis, VDR expression in the cytosol actually increased and thereby influenced the formation of the RIPK1/3 complex and subsequently relieved inflammatory responses by inhibiting necroptosis. This process might be a negative loop through which intestinal epithelial cells protect themselves in the case of colitis.…”

Section: F I G U R Ementioning

confidence: 99%

“…In addition, the key components in the necroptotic pathway, such as caspase-8 and RIPK3, play multi-directional roles in the balance between the pathways of cell death and process of tissue repair. 35 Our future studies will focus on the specific role of each necroptotic protein in response to vitamin D treatment in colitis.…”

Section: F I G U R Ementioning

confidence: 99%

Intestinal vitamin D receptor signaling ameliorates dextran sulfate sodium‐induced colitis by suppressing necroptosis of intestinal epithelial cells

et al. 2020

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Vitamin D status is closely related to inflammatory bowel disease (IBD), but the mechanism has not been fully elucidated. This study explored the effect of intestinal vitamin D signaling on necroptosis and the underlying mechanism in colitis. Serum 25(OH)D levels and the expression of necroptotic proteins were examined in patients with IBD. Colitis was induced in an intestinal‐specific hVDR transgenic model, and the gross manifestation, histological integrity, and intestinal barrier function were tested. The findings were further confirmed in vitro. Immunoprecipitation and colocalization were performed to investigate the association between the vitamin D receptor and necroptotic proteins. We found that serum 25(OH)D decreased in patients with IBD, while the expression of necroptotic proteins increased. The intestinal hVDR transgenic model could largely ameliorate the structural destruction, restore barrier dysfunction, and suppress necroptosis caused by DSS. This was probably achieved by binding to RIPK1/3 necrosomes, as we observed decreased RIPK1/3 necrosome formation and increased VDR expression in the cytosol. This study demonstrated an inhibitory effect of the intestinal vitamin D signaling pathway on necroptosis in DSS‐induced colitis. The vitamin D receptor shifts from the nucleus to the cytosol to impede the formation of RIPK1/3. Our findings may offer some theoretical basis for a novel treatment of IBD in clinical practice.

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RIPK1 binds to vitamin D receptor and decreases vitamin D-induced growth suppression

Cited by 4 publications

References 46 publications

Vitamin D Status Among Children aged 2 months to 13 years-a Hospital Based Study

Vitamin D Status Among Children aged 2 months to 13 years-a Hospital Based Study

Crosstalk between regulated necrosis and micronutrition, bridged by reactive oxygen species

Intestinal vitamin D receptor signaling ameliorates dextran sulfate sodium‐induced colitis by suppressing necroptosis of intestinal epithelial cells

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