Exposing partially ripe fruit to 1-methylcyclopropene (1-MCP) before or after cutting may be a useful supplement to proper temperature and relative humidity management for maintaining quality of fresh-cut fruit products. In this study tomato fruits were exposed to 0.5 ppm of 1-MCP for 24 hours, while tomato slices were exposed to the same concentration of 1-MCP for 6 hours. Untreated slices were used as control. Initially and after 3, 7, and 9 days of storage at 5 °C the following quality attributes were evaluated: flesh and skin color, firmness, total soluble solids content, titratable acidity and weight loss. In addition, respiration rate and ethylene production were measured. Fresh-cut tomato slices treated with 0.5 ppm of 1-MCP before cutting showed higher firmness retention than untreated slices, while slices treated after cutting showed an intermediate firmness value. Color development was delayed in both 1-MCP treated samples, which presented higher skin and flesh hue angle value compared with untreated slices. The initial decrease in skin hue angle value was reduced in slices treated either before or after cutting, while those treated after cutting showed the highest value of hue angle of the flesh. Application of 1-MCP did not affect the respiration rate, but slowed down C 2 H 4 production in slices treated after cutting, compared to slices from untreated tomatoes. No significant effect of 1-MCP treatment was observed on titratable acidity, while for soluble solids content slices treated after cutting showed a value significantly higher than untreated slices. Application before processing resulted most effective for firmness retention, while all other effects were more visible when application followed cutting.