Ripe fruits of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells

Son, Young‐Ok; Kim, J; Lim, Ji-Youn; Chung, Yong Ji; Chung, Gook-Hyun; Lee, J.-C

doi:10.1016/s0278-6915(03)00161-3

Cited by 136 publications

(102 citation statements)

References 26 publications

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“…The whole nightshade plant contains numerous steroid alkaloids, including solamargine, solasonine, solanine and saponin, and may be used for antitumor therapeutics, as it has a clear inhibitory effect on tumor growth in animal models and a toxic effect on tumor cells (14,15). The ethanol extract of nightshade is able to inhibit the growth of breast cancer and induce apoptosis in tumor cells (16).…”

Section: Introductionmentioning

confidence: 99%

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Meng

Zhang

et al. 2016

Oncology Letters

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Abstract. The aim of the present study was to investigate the effect of solanine on promoting human hepatocellular carcinoma HepG2 cells to produce reactive oxygen species (ROS), and the molecular mechanisms leading to tumor cell apoptosis. Solanine was administered to HepG2 cells in vitro. A selection of probes targeting various cellular localizations of ROS were used to detect ROS expression using flow cytometry. The expression levels of apoptosis-associated proteins, including apoptosis signal-regulating kinase 1 (ASK1) and thioredoxin binding protein 2 (TBP-2), and proliferation-associated proteins, including histone deacetylase 1 (HDAC1), were detected using western blotting. The percentage of cells undergoing apoptosis was measured using an Annexin V-fluorescein isothiocyanate/propidium iodide assay, and cell morphology was examined using Wright's stain followed by inverted microscopy analysis. ROS detection probes 2',7'-dichlorofluorescin diacetate and dihydrorhodamine 123 identified that abundant ROS, including hydroxyl radical (OH -) and hydrogen peroxide (H 2 O 2 ), were produced in the cytoplasm and mitochondria of the solanine-treated HepG2 cells compared with the control cells (P<0.05). Superoxide anion specific probes dihydroethidium and MitoSOX™ demonstrated that there were no significant alterations in the HepG2 cells following solanine treatment compared with the control cells (P>0.05). Western blotting results revealed that solanine upregulated the expression levels of ASK1 and TBP-2 and enhanced their kinase activities, whereas solanine decreased the expression level of the proliferation-associated protein, HDAC1. The cell apoptotic rate was significantly increased (P<0.0001) in the solanine-treated HepG2 cells compared with the control cells. (P<0.05). Overall, the study indicated that solanine induces HepG2 cells to produce ROS, mainly OH -and H 2 O 2 , in a mitochondria-dependent and -independent manner. In addition, solanine stimulates the expression of ASK1 and TBP-2, and their kinase activities, but inhibits the expression of proliferation-associated proteins, such as HDAC1, thus contributing to HepG2 cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Meng

Zhang

et al. 2016

Oncology Letters

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“…Solamargine (SM), a member of the Solanaceae (or nightshade) family, is extracted from the Chinese herb Solanum incanum L. and is a major steroidal glycoalkaloids (16). Previous studies have demonstrated that SM strongly inhibits the growth and induces the apoptosis of several human tumor cells, including breast, prostate, colon, lung and hepatoma cancer cells (17)(18)(19). Therefore, its marked antitumor activity has received increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Solamargine triggers hepatoma cell death through apoptosis

et al. 2015

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Abstract. Solamargine (SM), a steroidal alkaloid glycoside extracted from the traditional Chinese herb Solanum incanum, has been evidenced to inhibit the growth and induce apoptosis in a number of human cancer cell lines. In the present study, the anticancer effect of SM and underlying molecular mechanism of SM-induced apoptosis were investigated on the human hepatocellular carcinoma cells, SMMC7721 and HepG2. The proliferation effects of SM on the SMMC7721 and HepG2 cell lines were evaluated using MTT and colony formation assays. In addition, the percentage of apoptosis was measured using an Annexin V/propidium iodide staining method and the cell cycle distribution mediated by SM was analyzed using flow cytometry. The expression levels of B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3, caspase-9, proliferating cell nuclear antigen (pcna) and Ki67 proteins were examined to further demonstrate the proliferate and apoptosis effects of SM on the hepatoma cells. The results indicated that SM effectively inhibited hepatoma cell proliferation and promoted apoptosis. SM resulted in cell cycle arrest at the G 2 /M phase in the two cell lines. In addition, SM downregulated the levels of proliferation-associated (Ki67 and pcna) and anti-apoptotic (Bcl-2) proteins, and promoted the activity of apoptosis-associated proteins (Bax, caspase-3 and caspase-9). Therefore, the activation of the Bcl-2/Bax and caspase signaling pathways may be involved in the SM-induced apoptosis of hepatoma cells.

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“…Compared with the negative control group, SNL each dose group can significantly improve the S180 tumor-bearing mice red cell surface sialic acid content, including high-dose group SNL and SNL dose group had significant difference (p<0.05), SNL each dose group enhanced H 22 tumor-bearing mice can increase red cell surface sialic acid content. In summary SNL can significantly improve the sialic acid content of red blood cell membrane, thereby enhancing the body's red blood cells of tumor-bearing mice to identify, adhesion and transport function, prevent loss of sialic acid from the surface of the red cell membrane, restoring tumor-bearing mice red cell membrane function to achieve anti-tumor effect (Son et al, 2003).…”

Section: Resultsmentioning

confidence: 97%

Solanum Nigrum Polysaccharide (SNL) Extract Effects in Transplanted Tumor-bearing Mice - Erythrocyte Membrane Fluidity and Blocking of Functions

Yuan¹,

Liu²

2015

Asian Pacific Journal of Cancer Prevention

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Background: Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing S 180 and H 22 in mice. Materials and Methods: Fluorescencelabeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Results: Solanum nigrum polysaccharide could significantly improve the S 180 and H 22 tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then S 180 tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). H 22 tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

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Ripe fruits of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells

Cited by 136 publications

References 26 publications

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells

Solamargine triggers hepatoma cell death through apoptosis

Solanum Nigrum Polysaccharide (SNL) Extract Effects in Transplanted Tumor-bearing Mice - Erythrocyte Membrane Fluidity and Blocking of Functions

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