RINGO C is required to sustain the spindle-assembly checkpoint

Mourón, Silvana; Cárcer, Guillermo de; Seco, Esther; Fernández-Miranda, Gonzalo; Malumbres, Marcos; Nebreda, Ángel R.

doi:10.1242/jcs.078493

Cited by 2 publications

(2 citation statements)

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“…Three-dimensional (3D) image stacks were collected for each nucleus analyzed at 0.2-m Z-spacing. Fluorescence intensity quantification for staining of histone modifications was completed in Slidebook by a method similar to those used previously by other groups in a variety of experimental systems (25,52,73). Images were collected by setting exposure times such that the fluorescence intensity for each channel fell within the dynamic range of detection (approximately 2/3 of the maximal intensity for the sample).…”

Section: Methodsmentioning

confidence: 99%

“…We analyzed intestinal nuclei, which are 32-ploid, facilitating easier visualization and quantification of antibody staining. We quantified the ratio of average histone modification staining on X chromosomes versus the entire nucleus using intensity-based masks (see Materials and Methods), which is similar to methods used previously in other systems (25,52,73). Because dosage compensation in C. elegans involves a downregulation of gene expression on both hermaphrodite X chromosomes, we expected some activating histone modifications to be underrepresented or some repressive modifications to be enriched or a combination of the two.…”

Section: Dosage Compensation-dependent Changes In Chromatinmentioning

confidence: 99%

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Caenorhabditis elegans Dosage Compensation Regulates Histone H4 Chromatin State on X Chromosomes

et al. 2012

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Dosage compensation equalizes X-linked gene expression between the sexes. This process is achieved in Caenorhabditis elegans by hermaphrodite-specific, dosage compensation complex (DCC)-mediated, 2-fold X chromosome downregulation. How the DCC downregulates gene expression is not known. By analyzing the distribution of histone modifications in nuclei using quantitative fluorescence microscopy, we found that H4K16 acetylation (H4K16ac) is underrepresented and H4K20 monomethylation (H4K20me1) is enriched on hermaphrodite X chromosomes in a DCC-dependent manner. Depletion of H4K16ac also requires the conserved histone deacetylase SIR-2.1, while enrichment of H4K20me1 requires the activities of the histone methyltransferases SET-1 and SET-4. Our data suggest that the mechanism of dosage compensation in C. elegans involves redistribution of chromatin-modifying activities, leading to a depletion of H4K16ac and an enrichment of H4K20me1 on the X chromosomes. These results support conserved roles for histone H4 chromatin modification in worm dosage compensation analogous to those seen in flies, using similar elements and opposing strategies to achieve differential 2-fold changes in X-linked gene expression.

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Section: Methodsmentioning

confidence: 99%

Section: Dosage Compensation-dependent Changes In Chromatinmentioning

confidence: 99%