Rifomycin. XI. taxonomic study on streptomyces mediterranei nov. sp.

Margalith, P.; Beretta, G.

doi:10.1007/bf02089930

Cited by 63 publications

(33 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…mediterranei was originally classified as Sreptomyces mediterranei in 1957 [18]. However, later in 1969, the suggestion was made that it should have been labeled Nocardia [19] because of its cell-wall composition of meso-diaminopimilic acid (meso-DAP) and arabinose with a lack of glycine (Supplementary information, Table S4).…”

Section: The Phylogenetic/taxonomic Characteristics Of Amycolatopsismentioning

confidence: 99%

Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism

et al. 2010

View full text Add to dashboard Cite

Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10 236 715 base pairs, is one of the largest prokaryotic genomes ever sequenced so far. Unlike the linear topology found in streptomycetes, this chromosome is circular, particularly similar to that of Saccharopolyspora erythraea and Nocardia farcinica, representing their close relationship in phylogeny and taxonomy. Although the predicted 9 228 protein-coding genes in the A. mediterranei genome shared the greatest number of orthologs with those of S. erythraea, it was unexpectedly followed by Streptomyces coelicolor rather than N. farcinica, indicating the distinct metabolic characteristics evolved via adaptation to diverse ecological niches. Besides a core region analogous to that common in streptomycetes, a novel 'quasi-core' with typical core characteristics is defined within the non-core region, where 21 out of the total 26 gene clusters for secondary metabolite production are located. The rifamycin biosynthesis gene cluster located in the core encodes a cytochrome P450 enzyme essential for the conversion of rifamycin SV to B, revealed by comparing to the highly homologous cluster of the rifamycin B-producing strain S699 and further confirmed by genetic complementation. The genomic information of A. mediterranei demonstrates a metabolic network orchestrated not only for extensive utilization of various carbon sources and inorganic nitrogen compounds but also for effective funneling of metabolic intermediates into the secondary antibiotic synthesis process under the control of a seemingly complex regulatory mechanism.

show abstract

Section: The Phylogenetic/taxonomic Characteristics Of Amycolatopsismentioning

confidence: 99%

Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In fact, both organisms were originally described as Streptomyces spp. (Margalith & Beretta, 1960;Labeda, 1987). On the parsimonious hypothesis that sporulation septation in S. coelicolor and fragmentation in other actinomycetes are indeed homologous processes, whiG would appear not to be centrally involved in either process, whereas the omnipresent whiB might play a more direct role.…”

Section: Discussionmentioning

confidence: 99%

Functional and evolutionary implications of a survey of various actinomycetes for homologues of two Streptomyces coelicolor sporulation genes

Soliveri¹,

Vijgenboom²,

Granozzi³

et al. 1993

Journal of General Microbiology

View full text Add to dashboard Cite

In Streptomyces coelicolor A3(2) the whiB and whiG genes are essential for sporulation, their deduced products being a possible transcriptional activator and an RNA polymerase sigma factor, respectively. In a survey of DNA from diverse actinomycetes by Southern blotting, all samples tested hybridized with whiB, but only those representing genera capable of producing sporulating aerial mycelium hybridized with whiG. It is postulated that whiB may play a more intimate role in hyphal fragmentation processes (including sporulation) than whiG. The whiB and whiG homologues (whiB-Stv and whiG-Stv) of Streptoverticillium griseocarneum were cloned and sequenced, and subjected to functional tests in S. coelicolor whiB and whiC mutants. The genes were closely similar, but not identical, to their S. coelicolor counterparts at the DNA and deduced protein levels, and both Stv. griseocarnum gene products could function well in S. coelicolor. However, studies with hybrid transcription units suggested that the promoter region of whiB-Stv is somewhat inefficient in S. coelicolor.

show abstract

“…This finding suggests that these strains might share a highly similar chaxalactin PKS, which is not surprising given the number of examples of similar biosynthetic gene clusters occurring in distinct actinomycetes often isolated from different geographical locations. For instance, the rifamycin biosynthesis gene cluster is present in Amycolatopsis mediterranei S699, a mutant of a strain isolated in France (Kim et al 1992;Margalith and Beretta 1960) and in Salinispora arenicola CNS-205, isolated from Palau (Gontang et al 2007;Schultz et al 2008;Wilson et al 2010). …”

Section: S Leeuwenhoekii Strain C34mentioning

confidence: 99%

The ‘gifted’ actinomycete Streptomyces leeuwenhoekii

Castro

Razmilic

Gomez-Escribano

et al. 2018

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Streptomyces leeuwenhoekii strains C34T , C38, C58 and C79 were isolated from a soil sample collected from the Chaxa Lagoon, located in the Salar de Atacama in northern Chile. These streptomycetes produce a variety of new specialised metabolites with antibiotic, anti-cancer and anti-inflammatory activities. Moreover, genome mining performed on two of these strains has revealed the presence of biosynthetic gene clusters with the potential to produce new specialised metabolites. This review focusses on this new clade of Streptomyces strains, summarises the literature and presents new information on strain C34T .

show abstract

Rifomycin. XI. taxonomic study on streptomyces mediterranei nov. sp.

Cited by 63 publications

References 3 publications

Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism

Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism

Functional and evolutionary implications of a survey of various actinomycetes for homologues of two Streptomyces coelicolor sporulation genes

The ‘gifted’ actinomycete Streptomyces leeuwenhoekii

Contact Info

Product

Resources

About