Rifampicin Increases Expression of Plant Codon-Optimized Bacillus thuringiensis δ-Endotoxin Genes in Escherichia coli

Singh, Vivek Kumar; Nain, Vikrant; Phanindra, Mullapudi Lakshmi Venkata; Sellamuthu, Gothandapani; Chhapekar, Sushil Satish; Sreevathsa, Rohini; Rao, K.R.S. Sambasiva; Kumar, Polumetla Ananda; Kumar, Awanish

doi:10.1007/s10930-022-10043-y

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…Purified Cry proteins have a number of potentially important applications (37). They can be used for crop protection against target insects devoid of live bacteria and bacterial products.…”

Section: Discussionmentioning

confidence: 99%

Purified cytosolic crystals from Bacillus thuringiensis as a novel active pharmaceutical ingredient (API)

Chicca

Cazeault

Rus

et al. 2022

Preprint

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Bacillus thuringiensis or Bt is a Gram-positive soil bacterium, widely and safely applied in the environment as an insecticide for combatting insect pests that damage crops and vector diseases. Dominant active ingredients made by Bt are insect-killing crystal (Cry) proteins released as crystalline inclusions upon bacterial sporulation. Some Bt Cry proteins, e.g., Cry5B, target nematodes (roundworms) and show exceptional promise as anthelmintics (cures for parasitic nematode diseases). We have recently described IBaCC (for Inactivated Bacteria with Cytosolic Crystal(s)) in which bioactive Bt Cry crystals (containing Cry5B) are fully contained within the cytosol of dead bacterial ghosts. Here we demonstrate that these IBaCC-trapped Cry5B crystals can be liberated and purified away from cellular constituents yielding Purified Cytosolic Crystals (PCC). Cry5B PCC contains ~95% Cry5B protein out of the total protein content. Cry5B PCC is highly bioactive against parasitic nematode larvae and adults in vitro. Cry5B PCC is also highly active in vivo against experimental human hookworm and Ascaris infections in rodents. The process was scaled up to the 100 liter scale to produce PCC for a pilot study to treat two foals infected with the Ascarid, Parascaris spp. Single dose Cry5B PCC brought the fecal egg counts of both foals to zero. These studies describe the process for the scalable production of purified Bt crystals and define a new active pharmaceutical ingredient form of Bt Cry proteins.

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“…Purified Cry proteins have a number of potentially important applications (37). They can be used for crop protection against target insects devoid of live bacteria and bacterial products.…”

Section: Discussionmentioning

confidence: 99%

Purified cytosolic crystals from Bacillus thuringiensis as a novel active pharmaceutical ingredient (API)

Chicca

Cazeault

Rus

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Purified Cry proteins have a number of potentially important applications ( 36 ). They can be used for crop protection against target insects devoid of live bacteria and bacterial products.…”

Section: Discussionmentioning

confidence: 99%

Efficient and Scalable Process to Produce Novel and Highly Bioactive Purified Cytosolic Crystals from Bacillus thuringiensis

et al. 2022

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Transcriptomic analysis approach towards an improved tolerance of Escherichia coli to gallic acid stress

Liu,

Tang,

Liu

et al. 2023

Arch Microbiol

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Rifampicin Increases Expression of Plant Codon-Optimized Bacillus thuringiensis δ-Endotoxin Genes in Escherichia coli

Cited by 3 publications

References 37 publications

Purified cytosolic crystals from Bacillus thuringiensis as a novel active pharmaceutical ingredient (API)

Purified cytosolic crystals from Bacillus thuringiensis as a novel active pharmaceutical ingredient (API)

Efficient and Scalable Process to Produce Novel and Highly Bioactive Purified Cytosolic Crystals from Bacillus thuringiensis

Transcriptomic analysis approach towards an improved tolerance of Escherichia coli to gallic acid stress

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