Rickettsial agents detected in the genus Psathyromyia (Diptera:Phlebotominae) from a Biosphere Reserve of Veracruz, Mexico

Lozano-Sardaneta, Yokomi N; Valderrama, Anayansí; Sánchez‐Montes, Sokani; Grostieta, Estefanía; Colunga‐Salas, Pablo; Sánchez‐Cordero, Víctor; Becker, Ingeborg

doi:10.1016/j.parint.2021.102286

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…The association of the Wolbachia wWhi strain with Pa. shannoni has previously been recorded in Mexico (Lozano-Sardaneta, Valderrama et al, 2021), but our current work shows the highest MIR reported to date in females and males. This could suggest that wWhi strain is vertically transmitted in this species.…”

Section: Pathogen Detection In Sand Fliescontrasting

confidence: 50%

“…DNA was extracted individually from the collected sand flies (females and males) using the Chelex‐100 protocol (Lozano‐Sardaneta, Valderrama, et al, 2021). The DNA was analysed in pools of 10 specimens, which were grouped according to their taxonomic identification (species level), sex (female or male), date and locality.…”

Section: Methodsmentioning

confidence: 99%

“…For the detection of Leishmania spp., we amplified an ~350 bp fragment of the ribosomal ITS‐1 region using the primers LITSR (5′‐CTG GAT CAT TTT CCG ATG‐3′) and L5.8S (5′‐TGA TAC CAC TTA TCG CAC TT‐3′) and the previously described PCR conditions (Lozano‐Sardaneta et al, 2020). We amplified a fragment of the surface protein wsp of ~600 bp for the molecular detection of Wolbachia strains, using the primers wsp 81F (5′‐TGG TCC AAT AAG TGA TGA AGA AAC‐3′) and wsp 691R (5′‐AAA AAT TAA ACG CTA CTC CA‐3′) and previously reported PCR conditions (Lozano‐Sardaneta, Valderrama, et al, 2021). For the detection of Bartonella spp., a segment of ~378 bp of the citrate synthase ( glt A) gene was amplified using the primers BhCS871.p (5′‐GGG GAC CAG CTC ATG GTG G‐3′) and BhCS1137.n (5′‐AAT GCA AAA AGA ACA GTA AAC A‐3′), following the PCR conditions previously specified (Lozano‐Sardaneta et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

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Species diversity and detection of pathogens in phlebotomine sand flies collected from forest management areas of Quintana Roo, Mexico

Martínez‐Burgos,

Lozano‐Sardaneta,

Rodríguez‐Rojas

et al. 2023

Medical Vet Entomology

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Sand flies have expanded their areas of distribution, thereby increasing the risk of pathogen transmission in non‐endemic areas. To establish efficient prevention and control strategies for the transmission of vector‐borne pathogens, it is important to understand seasonal dynamics of their vectors. In Mexico, there are several areas where the contact between sand flies, hosts and reservoirs favours the transmission of the pathogen. We compared sand fly communities in a forest management area and a conserved area in Noh‐Bec, Quintana Roo, Mexico. The analysis included species diversity, activity peaks and molecular detection of pathogens. Sand flies were collected from November to December 2021 and April to May 2022, during 84 night‐traps. The conserved area showed higher numbers and greater species heterogeneity of sand flies as compared with the other sites. The β‐diversity analysis revealed that sites disturbed by logging (S1, S2, S3) had greater similarity (90%) in their sand fly species composition than a conserved area (S4) (similarity = 36%). Although none of the specimens were infected with Leishmania, we detected Wolbachia (19.4%) in all four sites, as well as Bartonella (3.25%) only in the disturbed sites. Further studies on the dynamics of sand fly populations and their association with pathogens are necessary.

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Section: Pathogen Detection In Sand Fliescontrasting

confidence: 50%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Species diversity and detection of pathogens in phlebotomine sand flies collected from forest management areas of Quintana Roo, Mexico

Martínez‐Burgos,

Lozano‐Sardaneta,

Rodríguez‐Rojas

et al. 2023

Medical Vet Entomology

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“…In Mexico, studies on microorganisms associated with sand flies are scarce. Yet, Wolbachia and Bartonella had previously been reported in sand flies (Mikery-Pacheco et al 2012;Lozano-Sardaneta et al 2019, 2021aLozano-Sardaneta et al 2021a, 2022. This led us to retrospectively analyze the possible presence of bacteria in sand flies species that had previously been collected in Chiapas, Mexico, a state with a high prevalence of leishmaniasis.…”

Section: Discussionmentioning

confidence: 99%

“…have recently been recorded in association with Lu. cruciata and Pa. shannoni in the states of Veracruz and Tamaulipas, Mexico, although it remains to be established whether the sand flies are vectors of these bacteria and if these Bartonella species cause emerging diseases (Lozano-Sardaneta et al 2019;Lozano-Sardaneta et al 2021a).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Wolbachia and Bartonella as part of the microbiome of phlebotomine sand flies from Chiapas, Mexico

et al. 2023

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Phlebotomine sand flies are dipterans of relevance due to their role as vectors of several pathogens worldwide. Bacteria in the gut of sand flies possibly affect their vectorial capacity and competence to transmit parasites. A retrospective study was performed in sand fly specimens that had previously been collected in four localities of the state of Chiapas during the period 2009–2011 to detect Wolbachia and Bartonella and their possible coinfection with Leishmania. For the molecular detection of bacteria, we used primers and conditions that had previously been reported. A total of 531 sand fly specimens of 10 species were analyzed. Four Wolbachia strains were detected in five sand fly species, showing a prevalence of 8.6%. All the Wolbachia strains had previously been reported in other taxa. In one sand fly species, we also detected a new lineage of Bartonella evidenced by a phylogenetic analysis. No sand fly specimens showed coinfections of these bacteria and Leishmania. The bacteria found in the phlebotomine sand flies are possibly transmitted by plant-mediated horizontal transmission and during blood meal feeding.

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Detection of Wolbachia and Leishmania DNA in sand flies (Diptera: Psychodidae, Phlebotominae) from a focus of cutaneous leishmaniasis in Tabasco, Mexico

et al. 2022

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Rickettsial agents detected in the genus Psathyromyia (Diptera:Phlebotominae) from a Biosphere Reserve of Veracruz, Mexico

Cited by 9 publications

References 44 publications

Species diversity and detection of pathogens in phlebotomine sand flies collected from forest management areas of Quintana Roo, Mexico

Species diversity and detection of pathogens in phlebotomine sand flies collected from forest management areas of Quintana Roo, Mexico

Molecular detection of Wolbachia and Bartonella as part of the microbiome of phlebotomine sand flies from Chiapas, Mexico

Detection of Wolbachia and Leishmania DNA in sand flies (Diptera: Psychodidae, Phlebotominae) from a focus of cutaneous leishmaniasis in Tabasco, Mexico

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