Rickettsia massiliae circulation in sheep and attached Rhipicephalus sanguineus in Central Portugal

Mesquita, João R.; Santos‐Silva, Sérgio; Moreira, Alícia de Sousa; Baptista, Maria Beatriz; Cruz, Rita de Cássia Ariza da; Esteves, Fernando; Vala, Helena; Barradas, Patrícia F.

doi:10.1007/s11250-022-03206-7

Cited by 2 publications

(3 citation statements)

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“…cornupunctata ticks infesting cattle, buffaloes, sheep, and goats were found to be positive for R. massiliae . Previously, R. massiliae has been detected in tick species of the genera Rhipicephalus , Haemaphysalis , Hyalomma , and Dermacentor , collected from dogs, small ruminants, and cattle in China, Iran, Nigeria, Tunisia, Portugal, Argentina, and the USA [ 17 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Rickettsia massiliae in Rh.…”

Section: Discussionmentioning

confidence: 99%

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

Shehla,

Ullah,

Alouffi

et al. 2023

Pathogens

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Ixodid ticks are responsible for the transmission of various intracellular bacteria, such as the Rickettsia species. Little Information is available about the genetic characterization and epidemiology of Rickettsia spp. The current study was designed to assess the tick species infesting various livestock hosts and the associated Rickettsia spp. in Pakistan. Ticks were collected from different livestock hosts (equids, cattle, buffaloes, sheep, goats, and camels); morphologically identified; and screened for the genetic characterization of Rickettsia spp. by the amplification of partial fragments of the gltA, ompA and ompB genes. Altogether, 707 ticks were collected from 373 infested hosts out of 575 observed hosts. The infested hosts comprised 105 cattle, 71 buffaloes, 70 sheep, 60 goats, 34 camels, and 33 equids. The overall occurrence of Rickettsia spp. was 7.6% (25/330) in the tested ticks. Rickettsia DNA was detected in Rhipicephalus haemaphysaloides (9/50, 18.0%), followed by Rhipicephalus turanicus (13/99, 13.1%), Haemaphysalis cornupunctata (1/18, 5.5%), and Rhipicephalus microplus (2/49, 4.1%); however, no rickettsial DNA was detected in Hyalomma anatolicum (71), Hyalomma dromedarii (35), and Haemaphysalis sulcata (8). Two Rickettsia agents were identified based on partial gltA, ompA, and ompB DNA sequences. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus showed 99–100% identity with Rickettsia sp. and Candidatus Rickettsia shennongii, and in the phylogenetic trees clustered with the corresponding Rickettsia spp. The Rickettsia species detected in Rh. haemaphysaloides, Rh. turanicus, Rh. microplus, and Ha. cornupunctata showed 100% identity with R. massiliae, and in the phylogenetic trees it was clustered with the same species. Candidatus R. shennongii was characterized for the first time in Rh. haemaphysaloides, Rh. turanicus, and Rh. microplus. The presence of SFG Rickettsia spp., including the human pathogen R. massiliae, indicates a zoonotic risk in the study region, thus stressing the need for regular surveillance.

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Section: Discussionmentioning

confidence: 99%

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

Shehla,

Ullah,

Alouffi

et al. 2023

Pathogens

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“…In animals, R. massiliae was previously detected in dog blood samples with splenic disease (Movilla et al, 2017) and in peripheral blood of camels (Selmi et al, 2020). Mesquita et al (2022) Candidatus R. barbariae, which is also a member of the spotted fever group, was first detected from Rhipicephalus bursa in Portugal, and named Rickettsia sp. PoTiRb169 (Sousa et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In animals, R. massiliae was previously detected in dog blood samples with splenic disease (Movilla et al, 2017) and in peripheral blood of camels (Selmi et al, 2020). Mesquita et al (2022) clarified the role of sheep as hosts in the maintenance, transmission, and enzootic circulation of this zoonotic agent. R. massiliae also is recognised as a human pathogen and may give rise to a clinical febrile illness with maculopapular rash, fever, night sweats, and headache (Garcia‐Garcia et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Detection of Rickettsia spp. and Anaplasma ovis in Melophagus ovinus from southern Xinjiang, China

Zhang

et al. 2023

Medical Vet Entomology

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Melophagus ovinus is a hematophagous insect that is distributed worldwide and plays a crucial role in transmitting disease‐causing pathogens. From June 2021 to March 2022, a total of 370 M. ovinus were collected from 11 sampling points in southern Xinjiang, China. The specimens were identified using morphological and molecular analyses. Rickettsia spp. and Anaplasma ovis were detected from all the samples using seven Rickettsia‐specific genetic markers and the msp‐4 gene of A. ovis. Approximately 11% of the M. ovinus specimens were positive for Rickettsia spp., and Candidatus Rickettsia barbariae was the most predominant species (35/41; 85.4%), while R. massiliae was least prevalent (6/41; 14.6%). Approximately 10.5% (39/370) of the M. ovinus specimens were positive for A. ovis of genotype III, which was co‐detected with Candidatus R. barbariae in M. ovinus (3/370; 0.8%). To the best of our knowledge, this is the first report of the detection of R. massiliae and Candidatus R. barbariae in M. ovinus globally. The detection and control of insect‐borne diseases originating from M. ovinus should be strengthened in southern Xinjiang, an area important to animal husbandry and production.

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Rickettsia massiliae circulation in sheep and attached Rhipicephalus sanguineus in Central Portugal

Cited by 2 publications

References 50 publications

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

Detection of Rickettsia spp. and Anaplasma ovis in Melophagus ovinus from southern Xinjiang, China

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