Rickettsia lusitaniae associated with Ornithodoros yumatensis (Acari: Argasidae) from two caves in Yucatan, Mexico

Sánchez‐Montes, Sokani; Guzmán-Cornejo, Carmen; Martínez-Nájera, Yecenia; Becker, Ingeborg; Venzal, José M.; Labruna, Marcelo B.

doi:10.1016/j.ttbdis.2016.09.003

Cited by 32 publications

(22 citation statements)

References 27 publications

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“…In order to identify the species of Bartonella and Rickettsia, we used the similarity criteria of the gltA and ompB genes proposed by La Scola (2003), Fournier and Roult (2009) and Fournier et al (2003). Global alignments were done using Clustal W (Thompson et al 1994) and the best substitution model was selected based on the lowest BIC (Bayesian Information Criterion) score for each gene using MEGA 6.0 (Tamura et al 2011;Sánchez-Montes et al 2016c). Additionally phylogenetic reconstruction was done using Maximum Likelihood also in MEGA 6.0 and branch support was evaluated over 10,000 bootstrap replications.…”

Section: Methodsmentioning

confidence: 99%

Detection of Bartonella and Rickettsia in small mammals and their ectoparasites in México

Sánchez‐Montes

Cabrera-Garrido

Ríos-Muñoz

et al. 2019

Therya

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Fleas and sucking lice are important vectors of multiple pathogens causing major epidemics worldwide. However these insects are vectors of a wide range of largely understudied and unattended pathogens, especially several species of bacteria's of the genera Bartonella and Rickettsia. For this reason the aim of the present work was to identify the presence and diversity of Bartonella and Rickettsia species in endemic murine typhus foci in Hidalgo, México. A cross-sectional study was carried out to collect small mammals and their associated ectoparasites during October, 2014. Samples of liver and ear of hosts, and ectoparasites were fixed in absolute ethanol and examined to identify the presence of Bartonella and Rickettsia DNA by the amplification of specific fragments of the gltA and ompB genes using conventional PCR. The recovered sequences were compared with those deposited in GenBank, and phylogenetic analyzes were carried out to identify the position of the pathogens detected with respect to the valid species previously reported worldwide. A total of 47 fleas and 172 sucking lice, belonging to five families (Ceratophyllidae, Leptopsyllidae, Ctenophtalmidae, Hoplopleuridae, Polyplacidae) and related to six species were collected from 40 rodents of four species and one shrew. Only four hosts (two P. beatae, and two R. norvergicus) were positive to Bartonella elizabethae, Bartonella vinsonii and Rickettsia typhi. In the case of ectoparasites, 23 specimens of two flea species (Peromyscopsylla hesperomys and Plusaetis mathesoni) tested positive for B. vinsonii. No evidence of Bartonella or Rickettsia was detected in any lice. Our findings represent the first record of Bartonella elizabethae a confirmed zoonotic pathogen causing endocarditis in México and several new associations of Bartonella with Mexican flea species, which highlight the importance of the establishment of active entomological surveillance in wildlife. Las pulgas y los piojos son vectores de patógenos causantes de epidemias de importancia histórica. Sin embargo, estos insectos son vectores de una amplia gama de patógenos poco estudiados y no atendidos, especialmente varias especies de bacterias de los géneros Bartonella y Rickettsia. Por este motivo, el objetivo del presente trabajo fue identificar la presencia y diversidad de las especies de Bartonella y Rickettsia en un foco de tifus murino en el estado de Hidalgo, México. Se realizó un estudio transversal para recolectar hospederos y sus ectoparásitos durante octubre de 2014. Las muestras de hígado y oreja de los hospederos y los ectoparásitos se fijaron en etanol absoluto y se examinaron para identificar la presencia de ADN de Bartonella y Rickettsia mediante la extracción de DNA y amplificación de fragmentos específicos de los genes gltA y ompB. Las secuencias obtenidas fueron comparadas con aquellas depositadas en GenBank y se realizaron análisis filogenéticos para identificar la posición de los patógenos detectados respecto a las especies válidas previamente reportadas a nivel mundial. Se re...

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Section: Methodsmentioning

confidence: 99%

Detection of Bartonella and Rickettsia in small mammals and their ectoparasites in México

Sánchez‐Montes

Cabrera-Garrido

Ríos-Muñoz

et al. 2019

Therya

Self Cite

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“…For the remaining specimens, we performed DNA extraction individually using the Chelex100 Chelating Resin (Bio-Rad, Hercules, CA, USA) protocol ( 10 ). For the initial screening, we amplified a conserved fragment of 805 bp of the gltA gene, which is present in all Rickettsia species ( 11 ). We characterized all positive samples by the amplification of 3 additional gene fragments ( ompA , ompB , and htrA ) using primers and conditions described elsewhere ( 11 , 12 ).…”

Section: The Studymentioning

confidence: 99%

“…For the initial screening, we amplified a conserved fragment of 805 bp of the gltA gene, which is present in all Rickettsia species ( 11 ). We characterized all positive samples by the amplification of 3 additional gene fragments ( ompA , ompB , and htrA ) using primers and conditions described elsewhere ( 11 , 12 ). The reaction mixture consisted of 12.5 μL of GoTaq Green Master Mix 2X (Promega, Madison, WI, USA), the corresponding pair of primers (100 ng each), 6.5 μL nuclease-free water, and 100 ng of DNA in a final volume of 25 μL.…”

Section: The Studymentioning

confidence: 99%

“…The reaction mixture consisted of 12.5 μL of GoTaq Green Master Mix 2X (Promega, Madison, WI, USA), the corresponding pair of primers (100 ng each), 6.5 μL nuclease-free water, and 100 ng of DNA in a final volume of 25 μL. In all reactions, we included a negative (reaction mix without DNA) and a positive (reaction mix with Rickettsia lusitaniae DNA detected in Ornithodoros yumatensis from a previous study in southern Mexico [ 11 ]) control. We sent amplicons of the expected size to Laboratorio de Biología Molecular y de la Salud, UNAM, for purification and sequencing.…”

Section: The Studymentioning

confidence: 99%

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Rickettsia parkeriinDermacentor parumapertusTicks, Mexico

Sánchez‐Montes¹,

López‐Pérez²,

Guzmán-Cornejo³

et al. 2018

Emerg. Infect. Dis.

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“…that were identical to those found in bats, indicating the vector-borne transmission of Rickettsia spp. [18][19][20][21][22][23]. So far, there is a lack of knowledge on Rickettsia spp.…”

Section: Introductionmentioning

confidence: 99%

Babesia vesperuginis in insectivorous bats from China

et al. 2018

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BackgroundTo increase understanding of human bacterial and parasitic pathogens in bats, we investigated the prevalence of Babesia spp., Rickettsia spp., Anaplasma spp. and Coxiella burnetii in bats from China.MethodsBats were captured from Mengyin County, Shandong Province of China using nets. DNA was extracted from the blood and spleen of bats for molecular detection of Babesia spp., Rickettsia spp., Anaplasma spp. and Coxiella burnetii with specific primers for each species.ResultsA total of 146 spleen samples and 107 blood samples of insectivorous bats, which belonged to 6 species within two families, were collected from Mengyin County, Shandong Province of China. We found that two Eptesicus serotinus (2/15, 13.3%) were positive for Babesia vesperuginis. We were unable to detect genomic sequences for Rickettsia spp., Anaplasma spp. and Coxiella burnetii.ConclusionsTo our knowledge, our study showed for the first time the presence of Babesia vesperuginis in Eptesicus serotinus collected from China, suggesting that Babesia vesperuginis has a broad host species and geographical distribution.

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Rickettsia lusitaniae associated with Ornithodoros yumatensis (Acari: Argasidae) from two caves in Yucatan, Mexico

Cited by 32 publications

References 27 publications

Detection of Bartonella and Rickettsia in small mammals and their ectoparasites in México

Detection of Bartonella and Rickettsia in small mammals and their ectoparasites in México

Rickettsia parkeriinDermacentor parumapertusTicks, Mexico

Babesia vesperuginis in insectivorous bats from China

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