Rickettsia japonica infections in Huanggang, China, in 2021

Li, Wei; Liu, Su-nan

doi:10.1016/j.idcr.2021.e01309

Cited by 9 publications

(3 citation statements)

References 16 publications

(21 reference statements)

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“…Rickettsia japonica has only been discovered once in north-eastern Europe [ 20 ], but the precise location, animal and tick source of the microorganism's DNA were not specified in this report. Rickettsia japonica is the causative agent of Japanese spotted fever (JSF), which is endemic in Japan [ 21 ]; in Europe, neither its vector, Haemaphysalis longicornis, nor a human case of JSF have ever been described.…”

Section: Resultsmentioning

confidence: 99%

Rickettsiales in the WHO European Region: an update from a One Health perspective

Guccione

Colomba

Iaria³

2023

Parasites Vectors

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Background The availability of molecular techniques has significantly increased our understanding of bacteria of the order Rickettsiales, allowing the identification of distinct species in both vector and host arthropods. However, the literature lacks studies that comprehensively summarize the vast amount of knowledge generated on this topic in recent years. The purpose of this study was to conduct a comprehensive analysis of the distribution of Rickettsiales in arthropod vectors, animals and humans in the WHO European Region in order to provide useful information to predict the emergence of certain diseases in specific geographical areas and to formulate hypotheses regarding the possible pathogenetic role of some rickettsial species in the etiology of human pathological conditions. Methods A systematic review of the literature in the PubMed and EMBASE databases was conducted following the PRISMA methodology using the search terms “Spotted fever” OR “rickettsiosis” OR “ricketts*” AND all the countries of the WHO European Region, from 1 January 2013 to 12 February 2022. Only studies that identified rickettsiae in human, animal or arthropod samples using molecular techniques were included in the review. Results A total of 467 articles considering 61 different species of Rickettsiales with confirmed or suspected human pathogenicity were analyzed in the review. More than 566 identifications of Rickettsiales DNA in human samples were described, of which 89 cases were assessed as importation cases. A total of 55 species of ticks, 17 species of fleas, 10 species of mite and four species of lice were found infected. Twenty-three species of Rickettsiales were detected in wild and domestic animal samples. Conclusion The routine use of molecular methods to search for Rickettsiales DNA in questing ticks and other blood-sucking arthropods that commonly bite humans should be encouraged. Molecular methods specific for Rickettsiales should be used routinely in the diagnostics of fever of unknown origin and in all cases of human diseases secondary to an arthropod bite or animal contact. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Rickettsiales in the WHO European Region: an update from a One Health perspective

Guccione

Colomba

Iaria³

2023

Parasites Vectors

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“…It was reported that the seroprevalence rate of anti-Rickettsia japonica antibody is about 21% among people in Yichang, a city in the Yiling Mountains region of central China (12). The main clinical symptoms of tick-borne rickettsioses also include fever and thrombocytopenia as well as headache, muscle pain, rash and local lymphadenopathy (13)(14)(15)(16) (12,17). It is worth noting that central China including the Dabie and Yiling Mountains region is also known to be an important endemic area for orthohantavirus hantanense (HTNV) infections transmitted by rodents, which belongs to the order Bunyavirales, family Hantaviridae, and genus Orthohantavirus (18).…”

Section: Introductionmentioning

confidence: 99%

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and orthohantavirus hantanense prevalent in central China

Wang,

Chen,

et al. 2024

Preprint

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Central China has been reported to be one of the most important endemic areas of zoonotic infection by spotted fever group rickettsiae(SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV) and orthohantavirus hantanense(HTNV). Due to similar clinical symptoms, it is challenging to make a definite diagnosis rapidly and accurately in the absence of microbiological tests. In the present study, an all-in-one real-time PCR assay was developed for the simultaneous detection of nucleic acids from SFGR, SFTSV and HTNV. Three linear standard curves for determining SFGR-ompA, SFTSV-Land HTNV-Lwere obtained within the range of 101-106copies/μL, with the PCR amplification efficiencies ranging from 93.46% to 96.88% and the regression coefficients R2of >0.99. The detection limit was 1.108 copies/μL for SFGR-ompA, 1.075 copies/μL for SFTSV-Land 1.006 copies/μL for HTNV-L, respectively. Both the within-run and within-laboratory coefficients of variation on the cycle threshold (Ct) values were within the range of 0.53%-2.15%. It was also found there was no statistical difference in the Ct values between with and without other non-target bloodborne virus nucleic acids (PSFGR-ompA=0.186, PSFTSV-L=0.612, PHTNV-L=0.298). The sensitivity, specificity, positive and negative predictive value were all 100% for determining SFGR-ompAand SFTSV-L, 97%, 100%, 100% and 99.6% for HTNV-L, respectively. Therefore, the all-in-one real-time PCR assay appears to be a reliable, sensitive, rapid, high-throughput and low cost-effective method to diagnose the zoonotic infection by SFGR, SFTSV and HTNV.Author SummarySpotted fever, severe fever with thrombocytopenia syndrome (SFTS), and hemorrhagic fever with renal syndrome (HFRS) sporadically have outbreaks in central China. Due to the similarities in clinical symptoms and the absence of reliable diagnostic methods, clinical diagnosis and treatment frequently result in misdiagnosis or missed diagnosis. Thus, the development of a fast and accurate diagnostic method is crucial for prevention and precise treatment. In this study, we designed an all-in-one real-time PCR assay to differentiate spotted fever group rickettsiae(SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV) and orthohantavirus hantanense(HTNV). The geneompAof SFGR, as well as the gene segmentLof SFTSV and HTNV, were used as targets to design primers and probes for amplification. Through the verification of nucleic acid and clinical sample detection, the sensitivity of this detection method exceeded 97%, and its specificity was 100%.This new assay could be applied in epidemiology and clinical diagnosis, to control new outbreaks, reduce diagnostic and identification time, and improve test efficiency.

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“…Spotted fever is caused by the spotted fever group Rickettsia species, which are mainly vectored and transmitted by ticks. In China, Japanese spotted fever (JSF) caused by Rickettsia japonica is a common disease in eastern and central areas such as Zhejiang, Hubei, and Henan provinces ( Lu et al., 2018 ; Li et al., 2019 ; Li and Liu, 2021 ; Gao et al., 2022 ; Zhou et al., 2023 ). The main manifestations of JSF are fever, erythema, and eschar ( Noguchi et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Survey of Rickettsia species in hematophagous arthropods from endemic areas for Japanese spotted fever in China

Tian,

Liu,

Liu

et al. 2024

Front. Cell. Infect. Microbiol.

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Japanese spotted fever (JSF) is caused by Rickettsia japonica, mainly vectored by hard ticks. However, whether R. japonica can be transmitted by other arthropods remains unknown. Moreover, it is of interest to investigate whether other Rickettsia species cause spotted fever in endemic areas. In this study, a survey of Rickettsia species was performed in hematophagous arthropods (mosquitoes, tabanids, and ticks) from endemic areas for JSF in Hubei Province, central China. The results showed that the diversity and prevalence of Rickettsia species in mosquitoes are low, suggesting that mosquitoes may not be the vector of zoonotic Rickettsia species. A novel Rickettsia species showed a high prevalence (16.31%, 23/141) in tabanids and was named “Candidatus Rickettsia tabanidii.” It is closely related to Rickettsia from fleas and mosquitoes; however, its pathogenicity in humans needs further investigation. Five Rickettsia species were identified in ticks. Rickettsia japonica, the agent of JSF, was detected only in Haemaphysalis longicornis and Haemaphysalis hystricis, suggesting that they may be the major vectors of R. japonica. Notably, two novel species were identified in H. hystricis ticks, one belonging to the spotted fever group and the other potentially belonging to the ancestral group. The latter one named “Candidatus Rickettsia hubeiensis” may provide valuable insight into the evolutionary history of Rickettsia.

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Rickettsia japonica infections in Huanggang, China, in 2021

Cited by 9 publications

References 16 publications

Rickettsiales in the WHO European Region: an update from a One Health perspective

Rickettsiales in the WHO European Region: an update from a One Health perspective

Development of an all-in-one real-time PCR assay for simultaneous detection of spotted fever group rickettsiae, severe fever with thrombocytopenia syndrome virus and orthohantavirus hantanense prevalent in central China

Survey of Rickettsia species in hematophagous arthropods from endemic areas for Japanese spotted fever in China

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