Rich extract on total polyphenols and antioxidant activity obtained by conventional and non-conventional methods from Ahmeur Bouamer grape seed

Ghouila, Z.; Laurent, Sophie; Henoumont, Céline; Elst, Luce Vander; Müller, Robert N.; Baâliouamer, Aoumeur

doi:10.4314/jfas.v8i3.3

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The DPPH and ABTS scavenging assays resulted in close values obtained through the two methods for the same organ and the same solvent but those obtained by DPPH method were slightly highest indicating a lower inhibition capacity compared to ABTS. Our observations are in line with those indicated by some authors which report that ABTS inhibition for samples from vegetables, fruits and beverages is higher than DPPH inhibition (Ghouila et al, 2016;Floegel et al, 2011). This is explained by the fact that the DPPH method is characterized by a lower sensitivity.…”

Section: Comparison Between Dpph and Abts Methodssupporting

confidence: 93%

Determination of Essential Oil Composition, Phenolic Content, and Antioxidant, Antibacterial and Antifungal Activities of Marigold (Calendula Officinalis L.) Cultivated In Algeria

2019

CRPJFST

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Microwave-assisted hydrodistillation (MAHD) was used as an ecofriendly method to extract the essential oils from flowers and leaves of Calendula officinalis L. cultivated in Algeria. The results obtained were compared with the conventional extraction method, hydrodistillation (HD), and analyzed by gas chromatography-flame ionization detector (GC-FID) and GC-MS. For flowers oils, 33 compounds were identified with HD method vs 20 compounds with MAHD method. For leaves, 26 compounds were identified with HD method vs 19 compounds identified with MAHD method. It is interesting to note, furthermore, that the use of MAHD method during 90 min allowed us to obtain relatively similar yields than HD method during 180 min. The main abundant volatile constituent was αcadinol with 31.9±0.71% for HD vs 39.7±0.26% for MAHD in leaves oils and 32.3±0.26% for HD vs 37.1±0.30% for MAHD in flowers oils. The oxygenated sesquiterpens was the most represented group of natural compounds contributing to the chemical composition in all oils. In the other hand, extraction of total phenolic compounds (TPC) and total flavonoids (TFC) was affected by the solvent type and, thus, 100% methanol was the better extraction solvent for both leaves and flowers. Highest levels were obtained from leaves. The highest antioxidant activity was recorded for leaves extract with 100% methanol. These values indicated a weak antioxidant activity compared to antioxidant standards. A correlation was established between the phenolic and flavonoids contents and the antioxidant activity of the crude extracts. A moderate to great antibacterial activity was observed against Gram+ bacteria. Any antibacterial activity was detected against fungi strains and Gram- bacteria.

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Section: Comparison Between Dpph and Abts Methodssupporting

confidence: 93%

Determination of Essential Oil Composition, Phenolic Content, and Antioxidant, Antibacterial and Antifungal Activities of Marigold (Calendula Officinalis L.) Cultivated In Algeria

2019

CRPJFST

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“…The other important factor for effective extraction of grape seeds is the type of technique used. A number of publications have described the application of both conventional and non-conventional extraction methods [22,23]. In the present study, experiments were carried out to extract antioxidants from Pinot Noir red grape seeds using two conventional techniques -a shaker and a magnetic stirrer.…”

Section: Resultsmentioning

confidence: 99%

“…The results obtained show that GSE is a strong ABTS +• radical cation inhibitor -2203.50 µM TE/g DW (551.515 mg TE/g DW). Guaita and Bosso [26] obtained lower ABTS results for seed extracts of different red grape species 109 -185 mg TE/g DW, but Ghouila et al [23] obtained higher 3902 µM TE/g DW. Li et al [24] received lower antioxidant capacity of seed extract from Cabernet Sauvignon -673.5 µM TE/g.…”

Section: Yeld Tpcmentioning

confidence: 93%

Optimization of the extraction procedure of polyphenols from red Pinot Noir grape seeds

Ivanov

2024

BIO Web Conf.

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Grape seed extract (GSE) in the last years is commercialized as a valuable ingredient in the food industry. The aims of this study are to optimize the extraction procedure of polyphenols from seeds of red Pinot Noir grape and to evaluate the phenolic content, antioxidant and antimicrobial potential of the obtained extract. Two conventional methods (with shaker and magnetic stirrer) and two non-conventional methods (with ultrasonic bath and microwave oven) were used for the extraction of the polyphenols from seeds. The optimal conditions for the production of GSE were - magnetic stirrer, 70% aqueous ethanol, 3 h, room temperature. The extraction yield and total phenol content of the obtained extract were 12% and 111.22 mg gallic acid/g dry weight, respectively. The contents of total flavonoids (TF), procyanidins (PC), total anthocyanins and ascorbic acid of the GSE were determined. The obtained GSE was rich in TF (51.50 mg quercetin/g) and PC (170.45 mg catechin/g). Antioxidant capacities of the extract were evaluated by 2,2-diphenyl-1-picrylhydrazyl (579.33 μM Trolox/g) and 2,2′-azino-bis (3ethylbenzothiazoline-6-sulfonic acid) assays (2203.50 μM Trolox/g). The extract has a high antimicrobial effect against B. cereus and B. subtilis. The obtained Pinot Noir GSE will be suitable additive for food products.

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“…The ABTS results ( Table 2 ) are similar to the results of other studies and show that GSEs are strong ABTS + radical cation inhibitors (1907.24–2273.90 µM TE/g DW). Guaita and Bosso (2019) [ 22 ] reported lower ABTS values for seed extracts of different red grape species—109–185 mg/g DW (or 434.91–738.15 µM TE/g DW) but Ghouila et al (2016) [ 48 ] showed higher values—3902 µM TE/g DW. Selkur et al (2011) [ 47 ] reported an antioxidant capacity determined by ABTS of 16.45 µM TE/g.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential, Antioxidant Activity, and Phenolic Content of Grape Seed Extracts from Four Grape Varieties

et al. 2023

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The aim of this study was to evaluate the total phenolic content, composition, and antioxidant and antibacterial activities of four grape seed extracts (Cabernet Sauvignon, Marselan, Pinot Noir, and Tamyanka). The total phenolic content (TPC) and flavonoid, anthocyanin, procyanidin, ascorbic acid, DPPH, and ABTS antioxidant capacities of the grape seed extracts (GSEs) were determined. The extracts showed high TPC values (79.06–111.22 mg GAE/g). The individual components in the GSEs were determined using HPLC. High contents of catechin, epicatechin, and procyanidin B1 were found in the extracts. The antimicrobial activity of the obtained GSEs against Staphylococcus aureus, Bacillus cereus, and Escherichia coli was evaluated using the agar diffusion test and a test to determine the minimum inhibitory concentration (MIC). According to the effect on the growth of pathogens, the extracts were ranked in the following order: Pinot Noir > Marselan > Cabernet Sauvignon > Tamyanka. The tested bacteria showed high sensitivity to the extracts (MIC = 0.12–0.50 mg/mL). According to the MIC values, the bacteria were in the following order: S. aureus > B. cereus > E. coli. A correlation was found between the phenolic content of the GSEs and their antibacterial potential. The obtained results show that the studied GSEs have good potential as antioxidant and antimicrobial agents.

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Rich extract on total polyphenols and antioxidant activity obtained by conventional and non-conventional methods from Ahmeur Bouamer grape seed

Cited by 4 publications

References 23 publications

Determination of Essential Oil Composition, Phenolic Content, and Antioxidant, Antibacterial and Antifungal Activities of Marigold (Calendula Officinalis L.) Cultivated In Algeria

Determination of Essential Oil Composition, Phenolic Content, and Antioxidant, Antibacterial and Antifungal Activities of Marigold (Calendula Officinalis L.) Cultivated In Algeria

Optimization of the extraction procedure of polyphenols from red Pinot Noir grape seeds

Antimicrobial Potential, Antioxidant Activity, and Phenolic Content of Grape Seed Extracts from Four Grape Varieties

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