Rice transcription factor WRKY114 directly regulates the expression of OsPR1a and Chitinase to enhance resistance against Xanthomonas oryzae pv. oryzae

Son, Seungmin; An, H. J.; Seol, Young-Joo; Park, Sang Ryeol; Im, Jong Hee

doi:10.1016/j.bbrc.2020.09.141

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…OsWRKY114 was grouped with WRKY Group III OsWRKY55 that reduces drought tolerance ( Figure 1 B). Moreover, a previous study showed that OsWRKY55 is one of the closest OsWRKY TFs to OsWRKY114 in amino acid sequences [ 24 ]. These results suggest that OsWRKY114 is involved in plant responses to drought stress as a negative regulator.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, we identified WRKY Group III OsWRKY114 as a positive regulator of rice resistance against Xoo [ 24 ]. To determine whether OsWRKY114 is also involved in abiotic stress, we treated drought stress to OsWRKY114 OX .…”

Section: Discussionmentioning

confidence: 99%

“…Taken together, our results suggest that OsWRKY114 negatively regulates drought tolerance through the inhibition of stomatal closure. Previously, we revealed that OsWRKY114 is a transcriptional activator and directly increases the gene expression of OsPR1a and chitinase [ 24 ]. However, since OsWRKY114 reduced the transcription levels of OsPYL2 and OsPYL10 , OsWRKY114 may regulate the OsPYLs expression indirectly.…”

Section: Discussionmentioning

confidence: 99%

“…Ilim) were used as the wild type in this study. OsWRKY114 -overexpressing rice plants ( OsWRKY114 OX ) were previously generated [ 24 ]. Before sowing, all kernels were sterilized in 2% sodium hypochlorite solution for 30 min and rinsed 5 times with sterilized distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study showed that OsWRKY114 enhances disease resistance to Xanthomonas oryzae pv. oryzae ( Xoo ) and directly activates the promoters of pathogenesis-related ( PR ) genes such as OsPR1a and chitinase in rice plants [ 24 ]. However, the function of OsWRKY114 in response to abiotic stress remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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OsWRKY114 Negatively Regulates Drought Tolerance by Restricting Stomatal Closure in Rice

Song

Son

Lee

et al. 2022

Plants

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The WRKY family of transcription factors plays a pivotal role in plant responses to biotic and abiotic stress. The WRKY Group III transcription factor OsWRKY114 is a positive regulator of innate immunity against Xanthomonas oryzae pv. oryzae; however, its role in abiotic stress responses is largely unknown. In this study, we showed that the abundant OsWRKY114 transcripts present in transgenic rice plants are reduced under drought conditions. The overexpression of OsWRKY114 significantly increased drought sensitivity in rice, which resulted in a lower survival rate after drought stress. Moreover, we showed that stomatal closure, which is a strategy to save water under drought, is restricted in OsWRKY114-overexpressing plants compared with wild-type plants. The expression levels of PYR/PYL/RCAR genes, such as OsPYL2 and OsPYL10 that confer drought tolerance through stomatal closure, were also markedly lower in the OsWRKY114-overexpressing plants. Taken together, these results suggest that OsWRKY114 negatively regulates plant tolerance of drought stress via inhibition of stomatal closure, which would otherwise prevent water loss in rice.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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