Rice Repetitive DNA Sequence RRD3: a Plant Promoter and Its Application to RNA Interference

Zhong, Jian; Wang, Hongbin; Zhang, Dangquan; Liu, Bing; Wang, Jinfa

doi:10.1016/s1673-8527(07)60027-7

Cited by 2 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recombinant binary plasmids used for RNAi-mediated silencing or overexpressing of Os-LYP4 and Os-LYP6 were designed as previously described (Zhong et al, 2007). In the RNAi constructs the sense and antisense fragments were separated by an intron element and expressed under the control of the Act1 promoter.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Lysin Motif–Containing Proteins LYP4 and LYP6 Play Dual Roles in Peptidoglycan and Chitin Perception in Rice Innate Immunity

et al. 2012

Self Cite

View full text Add to dashboard Cite

Plant innate immunity relies on successful detection of microbe-associated molecular patterns (MAMPs) of invading microbes via pattern recognition receptors (PRRs) at the plant cell surface. Here, we report two homologous rice (Oryza sativa) lysin motif-containing proteins, LYP4 and LYP6, as dual functional PRRs sensing bacterial peptidoglycan (PGN) and fungal chitin. Live cell imaging and microsomal fractionation consistently revealed the plasma membrane localization of these proteins in rice cells. Transcription of these two genes could be induced rapidly upon exposure to bacterial pathogens or diverse MAMPs. Both proteins selectively bound PGN and chitin but not lipopolysaccharide (LPS) in vitro. Accordingly, silencing of either LYP specifically impaired PGN-or chitin-but not LPS-induced defense responses in rice, including reactive oxygen species generation, defense gene activation, and callose deposition, leading to compromised resistance against bacterial pathogen Xanthomonas oryzae and fungal pathogen Magnaporthe oryzae. Interestingly, pretreatment with excess PGN dramatically attenuated the alkalinization response of rice cells to chitin but not to flagellin; vice versa, pretreatment with chitin attenuated the response to PGN, suggesting that PGN and chitin engage overlapping perception components in rice. Collectively, our data support the notion that LYP4 and LYP6 are promiscuous PRRs for PGN and chitin in rice innate immunity.

show abstract

Section: Plasmid Constructionmentioning

confidence: 99%

Lysin Motif–Containing Proteins LYP4 and LYP6 Play Dual Roles in Peptidoglycan and Chitin Perception in Rice Innate Immunity

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…The resulting 35S::OsPFA-DSP1 vector was introduced into Agrobacterium EHA105, and transformed into tobacco and rice as described (Jones et al 1995;Toki et al 2006). To obtain OsPFA-DSP1 RNAi rice plants, one fragment of sense and antisense OsPFA-DSP1 cDNA were cloned into the pRiAct1 vector (Zhong et al 2007), and then the promoter-RNAi fragment digested with KpnI and StuI was transferred into the pCAMBIA1301 binary vector cut with KpnI and PmlI. The primers are as follows: sense fragment, 5 0 -TCTAAGCTTGCGCTTTGCTGCTGCC-3 0 and 5 0 -ATA CTGCAGCCCGATGCATCCCTCT-3 0 , with HindIII and PstI sites (underlined), respectively; anti-sense fragment, 5 0 -TCTACGCGTGCGCTTTGCTGCTGCC-3 0 and 5 0 -ATA GTCGACCCCGATGCATCCCTCT-3 0 , with MluI and SalI sites (underlined), respectively.…”

Section: Generation Of Transgenic Tobacco and Ricementioning

confidence: 99%

OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants

et al. 2012

Self Cite

View full text Add to dashboard Cite

Dephosphorylation plays a pivotal role in regulating plant growth, development and abiotic/biotic stress responses. Here, we characterized a plant and fungi atypical dual-specificity phosphatase (PFA-DSP) subfamily member, OsPFA-DSP1, from rice. OsPFA-DSP1 was determined to be a functional protein tyrosine phosphatase (PTP) in vitro using phosphatase activity assays. Quantitative real-time PCR and GENEVESTIGATOR analysis showed that OsPFA-DSP1 mRNA was induced by drought stress. Transfection of rice protoplasts showed that OsPFA-DSP1 accumulated in both the cytoplasm and nucleus. Ectopic overexpression of OsPFA-DSP1 in tobacco increased sensitivity to drought stress and insensitivity to ABA-induced stomatal closure and inhibition of stomatal opening. Furthermore, overexpression of OsPFA-DSP1 in rice also increased sensitivity to drought stress. These results indicated that OsPFA-DSP1 is a functional PTP and may act as a negative regulator in drought stress responses.

show abstract

Rice Repetitive DNA Sequence RRD3: a Plant Promoter and Its Application to RNA Interference

Cited by 2 publications

References 27 publications

Lysin Motif–Containing Proteins LYP4 and LYP6 Play Dual Roles in Peptidoglycan and Chitin Perception in Rice Innate Immunity

Lysin Motif–Containing Proteins LYP4 and LYP6 Play Dual Roles in Peptidoglycan and Chitin Perception in Rice Innate Immunity

OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants

Contact Info

Product

Resources

About