Rice OsHsp16.9A interacts with OsHsp101 to confer thermotolerance

Liu, Yi‐Hsin; Tseng, Tong-Seung; Wu, Chia-Huang; Cho, Shu-Ting; Kuo, Chih-Horng; Huang, Xin-Jie; Cheng, Jung-Yi; Hsu, Kuo-Hsuan; Lin, Kung-Fu; Liu, Chia-Chin; Yeh, Ching-Hui

doi:10.1016/j.plantsci.2023.111634

Cited by 4 publications

(3 citation statements)

References 57 publications

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“…In this work, the DNA fragments of OsHsp16.9A and OsHsp18.0 for overexpression were cloned into the binary expression vector pCYH8 (Liu, et al 2023) in the downstream region of the ubiquitin 1 promoter (Ubi1). The recombinant constructs were then introduced into the pPZP/35H Ti-based vector, which was constructed by inserting 35S-hph-tml fragment into the HindIII site of pPZP200 (Supplementary material).…”

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confidence: 99%

“…The recombinant constructs were then introduced into the pPZP/35H Ti-based vector, which was constructed by inserting 35S-hph-tml fragment into the HindIII site of pPZP200 (Supplementary material). The recombinant constructs for overexpression of OsHsp16.9A and OsHsp18.0 were individually transferred into rice genome by Agrobacterium-mediated transformation (Liu, et al 2023) (Fig 1a). Two independent T3 homozygous transgenic lines OsHsp16.9A-OXs (OsHsp16.9A-OX1, and OsHsp16.9A-OX3) and OsHsp18.0-OXs (OsHsp18.0-OE1, and OsHsp18.0-OE3) were selected for heavy metal stress analysis.…”

Section: Full Textmentioning

confidence: 99%

“…Two independent T3 homozygous transgenic lines OsHsp16.9A-OXs (OsHsp16.9A-OX1, and OsHsp16.9A-OX3) and OsHsp18.0-OXs (OsHsp18.0-OE1, and OsHsp18.0-OE3) were selected for heavy metal stress analysis. Because OsHsp16.9A and OsHsp18.0 showed distinct resistance to high temperature in rice vegetative and reproductive stages (Liu, et al 2023). As compared with WT, constitute expression of OsHsp18.0-OXs leaves maintained higher chlorophyll content (p < 0.05, Fig.…”

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confidence: 99%

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Overexpression of OsHsp 18.0 in rice enhanced tolerance to heavy metal stress

Tseng,

Rajendran,

Liu

et al. 2023

Plant Cell Rep

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Plants synthesis multiple families of sHSPs with monomer sizes ranging from 12 to 42 kDa, are the most diverse and more abundant than in other organisms (Wu et al., 2022). In addition to heat tolerance, the sHSPs suggesting that they may play important roles other cellular processes under normal conditions (Wu et al., 2022). Over the past decades, it has been observed that the expression of certain plant sHSPs is induced by a range of stressors, including heat, salt, drought, osmotic, hormonal, heavy metal, oxidative stresses, as well as developmental signals speci c to plants (Wu, et al. 2022). In addition, a multitude of investigation have involved the overexpression of various sHSPs in both homologous and heterologous plant systems, including E. coli and Yeast models (Santhanagopalan, et al. 2015;Waters and Vierling 2020). The outcomes of many of these experiments have revealed that stress protection conferred by these proteins is restricted to speci c conditions and narrow range of plant growth stages. On contrast, suppression of CI or CII sHSPs in A. thaliana by RNAi showed that these sHSPs were required for recovery from a severe heat stress (10 h at 45°C) and have independent functions (McLoughlin, et al. 2016;Wu, et al. 2022). However, additional investigations are needed to uncover further applications for these proteins.

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Section: Full Textmentioning

confidence: 99%

Section: Full Textmentioning

confidence: 99%

Section: Full Textmentioning

confidence: 99%

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Overexpression of OsHsp 18.0 in rice enhanced tolerance to heavy metal stress

Tseng,

Rajendran,

Liu

et al. 2023

Plant Cell Rep

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Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species

Fan,

Qian,

et al. 2024

The Plant Genome

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The small heat shock proteins (sHSPs) are important components in plant growth and development, and stress response. However, a systematical understanding of the sHSP family is yet to be reported in five diploid Gossypium species. In this study, 34 GlsHSPs, 36 GrsHSPs, 37 GtsHSPs, 37 GasHSPs, and 38 GhesHSPs were identified in Gossypium longicalyx, Gossypium raimondii, Gossypium turneri, Gossypium arboreum, and Gossypium herbaceum, respectively. These sHSP members can be clustered into 10 subfamilies. Different subfamilies had different member numbers, motif distributions, gene structures, gene duplication events, gene loss numbers, and cis‐regulatory elements. Besides, the paleohexaploidization event in cotton ancestor led to expanding the sHSP members and it was also inherited by five diploid Gossypium species. After the cotton ancestor divergence, the sHSP members had the relatively conserved evolution in five diploid Gossypium species. The comprehensive evolutionary history of the sHSP family was revealed in five diploid Gossypium species. Furthermore, several GasHSPs and GhesHSPs were important candidates in plant growth and development, and stress response. These current findings can provide valuable information for the molecular evolution and further functional research of the sHSP family in cotton.

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