Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells

Fu, Ruifeng; Wang, Chong; Shen, Huan; Zhang, Jie; Higgins, James D.; Liang, Wanqi

doi:10.3389/fpls.2020.600820

Cited by 11 publications

(11 citation statements)

References 95 publications

(120 reference statements)

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“…Kumar reported that BRCA2 is a positive accelerator that provides the stabilizing functions and FIGL1 acts as a negative regulator to disassemble the filaments in Arabidopsis [ 41 ]. Fu also confirmed that OsBRCA2 is indispensable for facilitating the loading of OsRAD51 and OsDMC1 onto 3′ ssDNA of programmed DSBs during meiosis to facilitate single-end invasions of homologous chromosomes and precise recombination in rice [ 65 ]. Our data suggest that other proteins may mediate the functions of these two recombinases during rice meiosis.…”

Section: Discussionmentioning

confidence: 93%

OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis

Liu

Cao

et al. 2022

IJMS

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Meiotic recombination plays a pivotal role in achieving accurate chromosomal segregation and increasing genetic diversity. In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored. Here, we obtained different types of mutants for Osrad51a1, Osrad51a2, Osdmc1a, and Osdmc1b through CRISPR/Cas9. Both Osrad51a1 and Osrad51a2 exhibited normal vegetative growth and fertility. Osrad51 (Osrad51a1 Osrad51a2) mutant plants show normal vegetative growth but exhibit complete sterility, indicating that OsRAD51A1 and OsRAD51A2 are functionally redundant in rice fertility. In contrast to the wild type, Osrad51 chromosomes are not paired perfectly at pachytene and synaptonemal complex (SC) formation is deficient. Moreover, univalents and multivalent associations were observed at metaphase I, chromosome fragments presented at anaphase I, and crossover formation is basically suppressed in Osrad51 pollen mother cells (PMCs). OsRAD51 foci emerge at leptotene and disappear from late pachytene and chromosome localization of OsRAD51 depends on the formation of double-strand breaks (DSBs). Most OsRAD51 foci can co-localize with OsDMC1 signals. OsRAD51 is essential for the loading of OsDMC1 onto chromosomes, and vice versa. In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis. Moreover, the Osrad51 Osdmc1 (Osrad51a1 Osrad51a2 Osdmc1a Osdmc1b) quadruple mutant PMCs exhibited similar defective phenotypes as Osrad51 in homologous pairing, synapsis, and DSB repair. Taken together, our results suggest that the recombinases DMC1 and RAD51 may functionally depend on each other and play important roles in meiotic recombination during meiosis in rice.

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Section: Discussionmentioning

confidence: 93%

OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis

Liu

Cao

et al. 2022

IJMS

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“…Arabidopsis and rice brca2 mutants however show hypersensitivity to genotoxic agents suggesting repairing DNA damage and genomic instability Fu et al, 2020). Similar to their human homologs, both Arabidopsis and rice BRCA2 proteins interact with RAD51 and DMC1 (Siaud et al, 2004;Dray et al, 2006;Fu et al, 2020). During meiosis, Arabidopsis and rice mutants display a lack of RAD51 and DMC1 foci, incomplete synapsis, and chromosome fragmentation suggesting unrepair breaks (Fig.…”

Section: Brca2mentioning

confidence: 93%

“…Unlike the lethality caused by disruption of the BRCA2 gene in mammals that prevented genetic analysis, plant brca2 mutants are viable and grow without developmental defects. Arabidopsis and rice brca2 mutants however show hypersensitivity to genotoxic agents suggesting repairing DNA damage and genomic instability Fu et al, 2020). Similar to their human homologs, both Arabidopsis and rice BRCA2 proteins interact with RAD51 and DMC1 (Siaud et al, 2004;Dray et al, 2006;Fu et al, 2020).…”

Section: Brca2mentioning

confidence: 99%

Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators

2022

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Homologous recombination (HR) during meiosis is crucial for the DNA double-strand breaks (DSBs) repair that promotes the balanced segregation of homologous chromosomes and enhances genetic variation. In most eukaryotes, two recombinases RAD51 and DMC1 form nucleoprotein filaments on single-stranded DNA generated at DSB sites and play a central role in the meiotic DSB repair and genome stability. These nucleoprotein filaments perform homology search and DNA strand exchange to initiate repair using homologous template-directed sequences located elsewhere in the genome. Multiple factors can regulate the assembly, stability, and disassembly of RAD51 and DMC1 nucleoprotein filaments. In this review, we summarize the current understanding of the meiotic functions of RAD51 and DMC1 and the role of their positive and negative modulators. We discuss the current models and regulators of homology searches and strand exchange conserved during plant meiosis. Manipulation of these repair factors during plant meiosis also holds a great potential to accelerate plant breeding for crop improvements and productivity.

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“…RAD51C and XRCC3 facilitate the RAD51 chromosome localization ( Su et al, 2017 ; Jing et al, 2019 ; Zhang et al, 2020c ). RAD51 and DMC1 also associate with other proteins modulating their activities such as BRCA2 ( Siaud et al, 2004 ; Dray et al, 2006 ; Seeliger et al, 2012 ; Fu et al, 2020 ), FIGL1 and FLIP ( Zhang P. et al, 2017 ; Fernandes et al, 2018a ; Kumar et al, 2019 ), or MND1, and HOP2 ( Vignard et al, 2007 ; Uanschou et al, 2013 ; Aklilu et al, 2020 ). Rice MND1/MSF1 can interact with RPA2b and HOP2 ( Lu et al, 2020 ), while OsHOP2 can directly interact with the SC central element ZEP1/ZYP1 suggesting a second mechanism for the link observed between early recombination and synapsis ( Shi et al, 2019 ) at the SEI step.…”

Section: Single-end Invasions and Pairingmentioning

confidence: 99%

The Formation of Bivalents and the Control of Plant Meiotic Recombination

et al. 2021

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During the first meiotic division, the segregation of homologous chromosomes depends on the physical association of the recombined homologous DNA molecules. The physical tension due to the sites of crossing-overs (COs) is essential for the meiotic spindle to segregate the connected homologous chromosomes to the opposite poles of the cell. This equilibrated partition of homologous chromosomes allows the first meiotic reductional division. Thus, the segregation of homologous chromosomes is dependent on their recombination. In this review, we will detail the recent advances in the knowledge of the mechanisms of recombination and bivalent formation in plants. In plants, the absence of meiotic checkpoints allows observation of subsequent meiotic events in absence of meiotic recombination or defective meiotic chromosomal axis formation such as univalent formation instead of bivalents. Recent discoveries, mainly made in Arabidopsis, rice, and maize, have highlighted the link between the machinery of double-strand break (DSB) formation and elements of the chromosomal axis. We will also discuss the implications of what we know about the mechanisms regulating the number and spacing of COs (obligate CO, CO homeostasis, and interference) in model and crop plants.

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Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells

Cited by 11 publications

References 95 publications

OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis

OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis

Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators

The Formation of Bivalents and the Control of Plant Meiotic Recombination

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