Rice OsACDR1 (Oryza sativa Accelerated Cell Death and Resistance 1) Is a Potential Positive Regulator of Fungal Disease Resistance

Kim, Jung A; Cho, Kyoungwon; Singh, R. N.; Jung, Young‐Ho; Jeong, Seok–Hwan; Kim, So-Hee; Lee, Jae-Eun; Cho, Yoon-Seong; Agrawal, Ganesh Kumar; Rakwal, Randeep; Tamogami, Shigeru; Kersten, Birgit; Jeon, Jong‐Seong; An, Gynheung; Jwa, Nam‐Soo

doi:10.1007/s10059-009-0161-5

Cited by 69 publications

(56 citation statements)

References 30 publications

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“…A kinase assay was performed as described previously (Kim et al, 2009a). Briefly, 2 mg of recombinant purified protein in 25 mL of kinase buffer (25 mM Tris-HCl [pH 7.5], 0.2 mM EDTA, 5 mM MgCl 2 , 4 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, 0.1 mM Na 3 VO 4 , 10 mM NaF, and 10 mM b-glycerophosphate) was mixed with MBP (2 mg; artificial substrate, Invitrogen), 0.1 mM [g -32 P]ATP (5,000 cpm pmol 21 ), and 50 mm ATP and incubated at 30°C for 30 min.…”

Section: In Vitro Kinase Assaysmentioning

confidence: 99%

Rice Mitogen-Activated Protein Kinase Interactome Analysis Using the Yeast Two-Hybrid System

Singh

Lee

et al. 2012

Plant Physiology

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Mitogen-activated protein kinase (MAPK) cascades support the flow of extracellular signals to intracellular target molecules and ultimately drive a diverse array of physiological functions in cells, tissues, and organisms by interacting with other proteins. Yet, our knowledge of the global physical MAPK interactome in plants remains largely fragmented. Here, we utilized the yeast twohybrid system and coimmunoprecipitation, pull-down, bimolecular fluorescence complementation, subcellular localization, and kinase assay experiments in the model crop rice (Oryza sativa) to systematically map what is to our knowledge the first plant MAPK-interacting proteins. We identified 80 nonredundant interacting protein pairs (74 nonredundant interactors) for rice MAPKs and elucidated the novel proteome-wide network of MAPK interactors. The established interactome contains four membrane-associated proteins, seven MAP2Ks (for MAPK kinase), four MAPKs, and 59 putative substrates, including 18 transcription factors. Several interactors were also validated by experimental approaches (in vivo and in vitro) and literature survey. Our results highlight the importance of OsMPK1, an ortholog of tobacco (Nicotiana benthamiana) salicyclic acid-induced protein kinase and Arabidopsis (Arabidopsis thaliana) AtMPK6, among the rice MAPKs, as it alone interacts with 41 unique proteins (51.2% of the mapped MAPK interaction network). Additionally, Gene Ontology classification of interacting proteins into 34 functional categories suggested MAPK participation in diverse physiological functions. Together, the results obtained essentially enhance our knowledge of the MAPK-interacting protein network and provide a valuable research resource for developing a nearly complete map of the rice MAPK interactome.

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Section: In Vitro Kinase Assaysmentioning

confidence: 99%

Rice Mitogen-Activated Protein Kinase Interactome Analysis Using the Yeast Two-Hybrid System

Singh

Lee

et al. 2012

Plant Physiology

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“…The well-studied Raf-like MAPKKKs include Constitutive Triple Response1 (CTR1) and Enhanced Disease Resistance1 (EDR1), which negatively regulate ethylene and defense responses in Arabidopsis, respectively (Kieber et al, 1993;Frye et al, 2001). The rice (Oryza sativa) homolog Accelerated Cell Death and Resistance1/EDR1 was found to play a positive role in the regulation of disease resistance (Kim et al, 2009). Drought hypersensitive mutant1 (DSM1), another rice Raf-like MAPKKK, mediates drought resistance through ROS scavenging (Ning et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Compared with the numerous MAPK cascades documented in yeasts and animals, the complete MAPK cascades identified in plants are far fewer. One example is MEKK1-MKK4/MKK5-MPK3/MPK6-WRKY22/WRKY29, acting downstream of the flagellin receptor Flagellin Sensitive2; its function is to regulate plant defense responses (Asai et al, 2002;Kim et al, 2009). In plants, major challenges still remain in identification of individual kinases, especially due to the existence of more than one hundred components of plant MAPK cascades (Ichimura, 2002).…”

Section: Introductionmentioning

confidence: 99%

Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice

et al. 2011

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Mitogen-activated protein kinase kinase kinases (MAPKKKs), which function at the top level of mitogen-activated protein kinase cascades, are clustered into three groups. However, no Group C Raf-like MAPKKKs have yet been functionally identified. We report here the characterization of a rice (Oryza sativa) mutant, increased leaf angle1 (ila1), resulting from a T-DNA insertion in a Group C MAPKKK gene. The increased leaf angle in ila1 is caused by abnormal vascular bundle formation and cell wall composition in the leaf lamina joint, as distinct from the mechanism observed in brassinosteroidrelated mutants. Phosphorylation assays revealed that ILA1 is a functional kinase with Ser/Thr kinase activity. ILA1 is predominantly resident in the nucleus and expressed in the vascular bundles of leaf lamina joints. Yeast two-hybrid screening identified six closely related ILA1 interacting proteins (IIPs) of unknown function. Using representative IIPs, the interaction of ILA1 and IIPs was confirmed in vivo. IIPs were localized in the nucleus and showed transactivation activity. Furthermore, ILA1 could phosphorylate IIP4, indicating that IIPs may be the downstream substrates of ILA1. Microarray analyses of leaf lamina joints provided additional evidence for alterations in mechanical strength in ila1. ILA1 is thus a key factor regulating mechanical tissue formation at the leaf lamina joint.

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“…Increased sakuranetin accumulation and increased resistance against Magnaporthe grisea in ACDR1 overexpression lines (Kim et al, 2009). …”

Section: Metabolite Classmentioning

confidence: 99%

Metabolomics of Disease Resistance in Crops

Arbona

Gómez-Cádenas²

2016

Current Issues in Molecular Biology

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Plants are continuously exposed to the attack of invasive microorganisms, such as fungi or bacteria, and also viruses. To fight these attackers, plants develop different metabolic and genetic responses whose final outcome is the production of toxic compounds that kill the pathogen or deter its growth or semiotic molecules that alert other individuals of the same plant species. These molecules are derived from the secondary metabolism and their production is induced upon detection of a pathogen-associated molecular pattern (PAMP). These PAMPs are different molecules that are perceived by the host cell triggering defense responses. PAMP-elicited compounds are highly diverse and specific of every plant species and can be divided into preformed metabolites or phytoanticipins that are converted into toxic molecules upon pathogen perception, and toxic metabolites or phytoalexins that are produced only upon pathogen attack. Moreover, plant volatile emissions are also modified in response to pathogen attack to alert neighboring individuals or to make plants less attractive to pathogen vector arthropods. Plant metabolite profiling techniques have allowed the identification of novel antimicrobial molecules that are induced upon elicitation. However, more studies are required to assess the specific function of metabolites or metabolite blends on plant-microbe interactions.

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Rice OsACDR1 (Oryza sativa Accelerated Cell Death and Resistance 1) Is a Potential Positive Regulator of Fungal Disease Resistance

Cited by 69 publications

References 30 publications

Rice Mitogen-Activated Protein Kinase Interactome Analysis Using the Yeast Two-Hybrid System

Rice Mitogen-Activated Protein Kinase Interactome Analysis Using the Yeast Two-Hybrid System

Increased Leaf Angle1, a Raf-Like MAPKKK That Interacts with a Nuclear Protein Family, Regulates Mechanical Tissue Formation in the Lamina Joint of Rice

Metabolomics of Disease Resistance in Crops

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