Rice leaf associated Chryseobacterium species: An untapped antagonistic flavobacterium displays volatile mediated suppression of rice blast disease

Kumar, Mukesh; Charishma, K.; Sahu, Kuleshwar Prasad; Sheoran, Neelam; Patel, Asharani; Kundu, Aditi; Kumar, Aundy

doi:10.1016/j.biocontrol.2021.104703

Cited by 20 publications

(17 citation statements)

References 65 publications

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“…The sequence was then queried against the EzBioCloud database. Relevant type strains were selected to construct a phylogenetic tree using the maximum-likelihood method ( Kumar et al, 2021 ). The phylogenetic analysis revealed that the strain YC-9 and B. siamensis clustered on the same branch, with 99.86% sequence similarity for the 16S rRNA gene sequence ( Figure 3 ), indicating the strain YC-9 as B. siamensis .…”

Section: Resultsmentioning

confidence: 99%

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

Zhou

Wang

et al. 2022

Front. Microbiol.

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The antagonistic potential of bacteria obtained from the nest of Odontotermes formosanus was assessed against Fusarium oxysporum f. sp. cucumerinum (FOC). Of 30, seven termite nest-associated bacteria strains had biocontrol potential. Among them, the strain YC-9 showed the strongest antifungal activity toward FOC. Phylogenetic analysis of the 16S rRNA amplified product of YC-9 revealed its identification as Bacillus siamensis. The in vivo antifungal activity experiment showed that the application of YC-9 at 108 cfu/ml significantly reduced the cucumber wilt incidence with a control efficacy of 73.2%. Furthermore, plant growth parameters such as fresh weight, dry weight, plant height, and root height were significantly improved by 42.6, 53.0, 20.8, and 19.3%, respectively. We found that inoculation with B. siamensis YC-9 significantly increased the activity of defensive enzymes such as peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) in diseased cucumber roots, thereby raising the resistance. PCR using gene-specific primers revealed that B. siamensis YC-9 contains biosynthetic genes for known antibiotics, including bacillomycin, iturin, and surfactin. Chemical analysis of the cultivation of B. siamensis YC-9 resulted in the isolation of five metabolites, including hexadecanoic acid (1), cyclo-(L-phenylalanylglycine) (2), cyclo-(L-trans-Hyp-L-Leu) (3), C15-surfactin (4), and macrolactin A (5), the structures of which were identified by the analysis of NMR spectroscopic data and MS. Among them, the compound 4 showed significant antifungal activity against conidial germination of FOC with an IC50 value of 5.1 μg/ml, which was comparable to that of the positive control, cycloheximide (IC50 value of 2.6 μg/ml). Based on these findings, this study suggests that termite-nest associated B. siamensis YC-9 could be a potential biological control agent for integrated control of soil-borne diseases like cucumber Fusarium wilt.

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Section: Resultsmentioning

confidence: 99%

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

Zhou

Wang

et al. 2022

Front. Microbiol.

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“…Due to their competition with bacterial communities present in the rhizosphere, PGPB are regarded as a tool for sustainable farming and a trend in future agriculture. The PGPB group comprises many genera that have been studied extensively for many years, i.e., Acetobacter, Acinetobacter, Alcaligenes, Arthrobacter, Azoarcus, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Derxia, Enterobacter, Gluconacetobacter, Herbaspirillum, Klebsiella, Ochrobactrum, Pantoae, Paenarthrobacter, Pseudarthrobacter, Pseudomonas, Rhodococcus, Serratia, Stenotrophomonas, Zoogloea, Paenibacillus, Lysinibacillus and Staphylococcus [ 69 , 70 , 71 , 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

“…They use distinctive direct mechanisms facilitating plant growth, e.g., nutrient dissolution and delivery, nitrogen fixation, production of siderophores and phytohormones, and modulation of plant hormone levels. The indirect mechanisms include the production of exopolysaccharides, hydrogen cyanide and lytic enzymes, the formation of biofilm, antibiosis, and induced systemic immunity [ 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. As demonstrated by Tiwari (2019) [ 74 ], these mechanisms increase plant yields directly or indirectly.…”

Section: Resultsmentioning

confidence: 99%

Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

Pastuszczak

Stanek-Tarkowska

Kačániová

2022

IJERPH

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This study investigated the effect of different doses of fertilization with biomass combustion ash (Salix viminalis L. willow) on changes in the biological, chemical, and physical properties of soil. The experiment was carried out on podzolic and chernozem soils in a one-way field experiment (fertilization dose: control (without fertilization), NPK (nitrogen (N), phosphorus (P) and potassium (K)), 100, 200, 300, 400, 500 kg K2O ha−1). The biomass ash was characterized by a pH value of 12.83 ± 0.68 and a high content of macronutrients. The samples were collected from 0–5, 10–15, and 20–25 cm soil layers under the cultivation of spring barley (Hordeum vulgare L) cv. Planet in April and August 2021. Mass spectrometry (MALDI-TOF MS) was used for microbiological analyses, which revealed the presence of 53 culturable species from 11 genera: Bacillus, Pseudomonas, Paenibacillus, Lysinibacillus, Pseudarthrobacter, Arthrobacter, Staphylococcus, Paenarthrobacter, Micrococcus, Rhodococcus, and Flavobacterium. The podzolic and chernozem soils exhibited the presence of 28 and 44 culturable species, respectively. The study showed an increase in the number of microorganisms in the top layer of the soil profile. However, the number of bacteria decreased at the depths of 10–15 cm and 20–25 cm. With depth, the bulk density (BD) and moisture increased.

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“…In addition to probiotic effects on host plants, several endophytic bacteria have been reported to induce systemic response against pathogens in many dicots and monocot crops including rice ( Sheoran et al, 2015 , 2016 ; Agisha et al, 2017 ; Hirakue and Sugiyama, 2018 ; Li et al, 2019 ; Sarkar et al, 2019 ; Ashajyothi et al, 2020 ; Matsumoto et al, 2021 ; Patel et al, 2021 ). Recently, we published the antagonistic and host defense elicitation potential of rice-endophytic flavobacterial isolate Chryseobacterium endophyticum against rice blast ( Kumar et al, 2021a ). It is known that plant ecological functions including its defense against the pathogen are carried out by the synergistic effects of the plant microbiome, there are ample chances that other rice endophytic bacteria can have blasticidal potential.…”

Section: Introductionmentioning

confidence: 99%

Rice leaf endophytic Microbacterium testaceum: Antifungal actinobacterium confers immunocompetence against rice blast disease

et al. 2022

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Genetic and functional characteristics of rice leaf endophytic actinobacterial member, Microbacterium are described. Morphotyping, multilocus sequence analysis and transmission electron microscopy indicated the species identity of the endophytic bacterium, OsEnb-ALM-D18, as Microbacterium testaceum. The endophytic Microbacterium showed probiotic solubilization of plant nutrients/minerals, produced hydrolytic enzyme/phytohormones, and showed endophytism in rice seedlings. Further, the endophytic colonization by M. testaceum OsEnb-ALM-D18 was confirmed using reporter gene coding for green fluorescence protein. Microbacterium OsEnb-ALM-D18 showed volatilome-mediated antibiosis (95.5% mycelial inhibition) on Magnaporthe oryzae. Chemical profiling of M. testaceum OsEnb-ALM-D18 volatilome revealed the abundance of 9-Octadecenoic acid, Hexadecanoic acid, 4-Methyl-2-pentanol, and 2,5-Dihydro-thiophene. Upon endobacterization of rice seedlings, M. testaceum altered shoot and root phenotype suggestive of activated defense. Over 80.0% blast disease severity reduction was observed on the susceptible rice cultivar Pusa Basmati-1 upon foliar spray with M. testaceum. qPCR-based gene expression analysis showed induction of OsCERK1, OsPAD4, OsNPR1.3, and OsFMO1 suggestive of endophytic immunocompetence against blast disease. Moreover, M. testaceum OsEnb-ALM-D18 conferred immunocompetence, and antifungal antibiosis can be the future integrated blast management strategy.

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Rice leaf associated Chryseobacterium species: An untapped antagonistic flavobacterium displays volatile mediated suppression of rice blast disease

Cited by 20 publications

References 65 publications

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation

Rice leaf endophytic Microbacterium testaceum: Antifungal actinobacterium confers immunocompetence against rice blast disease

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