Rice Heavy Metal P-type ATPase OsHMA6 Is Likely a Copper Efflux Protein

Zou, Wenli; Chang, Li; Yingguo, Zhu; Chen, Jingguang; He, Hua; Ye, Guoyou

doi:10.1016/j.rsci.2020.01.005

Cited by 12 publications

(2 citation statements)

References 41 publications

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“…Previous studies have shown that the Cu chaperone OsATX1 plays an important role in trafficking Cu to the Cu transporters OsHMA4, OsHMA5, OsHMA6 and OsHMA9 to maintain Cu homoeostasis in different rice tissues (Zhang et al, 2018; Zou et al, 2020). Whether OsCOPT7 also interacts with OsATX1 was examined using a yeast two‐hybrid assay.…”

Section: Resultsmentioning

confidence: 99%

OsCOPT7 is a copper exporter at the tonoplast and endoplasmic reticulum and controls Cu translocation to the shoots and grain of rice

Tang,

Li,

Zhang

et al. 2024

Plant Cell & Environment

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Copper (Cu) is an essential micronutrient for all living organisms but is also highly toxic in excess. Cellular homoeostasis of Cu is maintained by various transporters and metallochaperones. Here, we investigated the biological function of OsCOPT7, a member of the copper transporters (COPT) family, in Cu homoeostasis in rice. OsCOPT7 was mainly expressed in the roots and the expression was upregulated by Cu deficiency. OsCOPT7 was localized at the tonoplast and the endoplasmic reticulum. Knockout of OsCOPT7 increased Cu accumulation in the roots but decreased Cu concentrations in the shoots and grain. The knockout mutants contained higher concentrations of Cu in the roots cell sap but markedly lower concentrations of Cu in the xylem sap than wild‐type plants. Seed setting and grain yield were reduced significantly in the knockout mutants grown in a low Cu soil. Knockout mutants were more tolerant to Cu toxicity. Yeast two‐hybrid and bimolecular fluorescence complementation assays showed that OsCOPT7 interacts physically with the rice Cu chaperone antioxidant protein 1 (OsATX1). Taken together, our results indicate that OsCOPT7 is a specific Cu transporter functioning to export Cu from the vacuoles and the ER and plays an important role in controlling the root‐to‐shoot Cu translocation in rice.

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Section: Resultsmentioning

confidence: 99%

OsCOPT7 is a copper exporter at the tonoplast and endoplasmic reticulum and controls Cu translocation to the shoots and grain of rice

Tang,

Li,

Zhang

et al. 2024

Plant Cell & Environment

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“…OsHAM9 is a copper efflux protein localized to the plasma membrane. It is expressed in roots and helps in lead, zinc and copper transport 42 , 43 .OsHMA2 and OsHMA5 are located at the plasma membrane and perform the function of heavy metal transport from root to shoot 44 , 45 . The earlier investigation also suggested that AtHMA7 is a post-golgi protein that delivers copper to an endoplasmic reticulum-associated protein (ethylene receptors ETR1).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of heavy metal ATPases (HMAs) in Poaceae species and their potential role against copper stress in Triticum aestivum

Batool

Aslam

Gul

et al. 2023

Sci Rep

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Plants require copper for normal growth and development and have evolved an efficient system for copper management based on transport proteins such as P1B-ATPases, also known as heavy metal ATPases (HMAs). Here, we report HMAs in eleven different Poaceae species, including wheat. Furthermore, the possible role of wheat HMAs in copper stress was investigated. BlastP searches identified 27 HMAs in wheat, and phylogenetic analysis based on the Maximum Likelihood method demonstrated a separation into four distinct clades. Conserved motif analysis, domain identification, gene structure, and transmembrane helices number were also identified for wheat HMAs using computational tools. Wheat seedlings grown hydroponically were subjected to elevated copper and demonstrated toxicity symptoms with effects on fresh weight and changes in expression of selected HMAs TaHMA7, TaHMA8, and TaHMA9 were upregulated in response to elevated copper, suggesting a role in wheat copper homeostasis. Further investigations on these heavy metal pumps can provide insight into strategies for enhancing crop heavy metal tolerance in the face of heavy metal pollution.

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Advanced Techniques in Omics Research in Relation to Heavy Metal/Metalloid Toxicity and Tolerance in Plants

Raza,

Bashir,

Salehi

et al. 2023

Heavy Metal Toxicity and Tolerance in Plants

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Rice Heavy Metal P-type ATPase OsHMA6 Is Likely a Copper Efflux Protein

Cited by 12 publications

References 41 publications

OsCOPT7 is a copper exporter at the tonoplast and endoplasmic reticulum and controls Cu translocation to the shoots and grain of rice

OsCOPT7 is a copper exporter at the tonoplast and endoplasmic reticulum and controls Cu translocation to the shoots and grain of rice

Genome-wide analysis of heavy metal ATPases (HMAs) in Poaceae species and their potential role against copper stress in Triticum aestivum

Advanced Techniques in Omics Research in Relation to Heavy Metal/Metalloid Toxicity and Tolerance in Plants

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