Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB

Ithal, Nagabhushana; Reddy, A. R.

doi:10.1016/j.plantsci.2004.02.002

Cited by 127 publications

(92 citation statements)

References 41 publications

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“…Balakumar et al (1993) relataram acúmulo desses compostos em resposta à seca. Em arroz, os genes OsDfr e OsAns, envolvidos na biossíntese de flavonoides, foram induzidos por seca, salinidade e ABA (Ithal & Reddy, 2004). A indução de VuCPRD14 nos nódulos em condições de estresse hídrico pode estar associada ao aumento na biossíntese de flavonoides, necessário para reduzir os danos oxidativos ocasionados pelo estresse.…”

Section: Resultsunclassified

Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi

Silva

Galisa

Oliveira

et al. 2012

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o efeito isolado ou simultâneo dos estresses hídrico e térmico na expressão gênica em nódulos de feijão-caupi. A bactéria Bradyrhizobium japonicum (estirpe BR 3267) foi inoculada em sementes de feijão-caupi da cultivar IPA 206 e, 35 dias após a germinação, as plantas foram submetidas a diferentes regimes de disponibilidade hídrica e a estresse térmico, em casa de vegetação. Para a identificação dos genes diferencialmente expressos, foi utilizada a técnica de cDNA-AFLP, tendo-se isolado 67 fragmentos derivados de transcritos (FDTs) diferencialmente expressos. Após o sequenciamento dos FDTs e das análises de similaridade, com uso do programa Blastx, foram identificados 14 genes diferencialmente expressos envolvidos em diferentes processos metabólicos. O padrão de expressão de seis genes sob estresse abiótico foi confirmado por RT-qPCR, e observou-se indução de genes pertencentes a diferentes categorias funcionais, como biossíntese de ácido abscísico, sinalização celular, transportador de prolina e biossíntese de lipídeos de membranas. A expressão desses genes indica sua participação em processos relacionados à proteção dos nódulos ao estresse abiótico.Termos para indexação: Vigna unguiculata, cDNA-AFLP, fixação biológica de nitrogênio, genômica funcional. Gene expression induced by abiotic stress in cowpea nodulesAbstract -The objective of this work was to evaluate the isolated or concomitant effect of drought and heat stresses on the gene expression of cowpea nodules. The bacterium Bradyrhizobium japonicum (strain BR 3267) was inoculated in cowpea seeds from the cultivar IPA 206 and, 35 days after germination, the plants were subjected to different water availability regimes and to heat stress under greenhouse conditions. To identify differentially expressed genes, the cDNA-AFLP technique was used, and 67 differentially expressed transcript-derived fragments (TDFs) were isolated. After TDFs sequencing and similarity analyses, using the Blastx program, 14 differentially expressed genes were identified, which are involved in different metabolic processes. The expression pattern of six genes under abiotic stress conditions was confirmed by RT-qPCR, and the induction of different functional gene categories, such as abscisic acid biosynthesis, cell signaling, proline transporter, and lipid membrane biosynthesis, was observed. The expression of these genes indicates their participation in processes related to nodule protection under abiotic stresses.Index terms: Vigna unguiculata, cDNA-AFLP, biological nitrogen fixation, functional genomics. IntroduçãoEm condições tropicais, a produtividade agrícola pode ser negativamente afetada por uma série de estresses bióticos e abióticos que alteram o crescimento e o desenvolvimento vegetal. Nesse contexto, destacamse a deficiência de nutrientes, como nitrogênio e fósforo, bem como os estresses decorrentes da baixa disponibilidade hídrica e das altas temperaturas.A fixação biológica de nitrogênio (FBN) é um processo complexo que pode ...

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Section: Resultsunclassified

Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi

Silva

Galisa

Oliveira

et al. 2012

Pesq. agropec. bras.

View full text Add to dashboard Cite

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“…The regulatory elements that control these processes are only beginning to be understood. Recent studies have found that the basic domain/leucine zipper family of transcription factors, together with the Myb genes, play a role in induced expression (Ithal and Reddy, 2004;Hartmann et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Caught Red-Handed:RcEncodes a Basic Helix-Loop-Helix Protein Conditioning Red Pericarp in Rice

et al. 2006

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Rc is a domestication-related gene required for red pericarp in rice (Oryza sativa). The red grain color is ubiquitous among the wild ancestors of O. sativa, in which it is closely associated with seed shattering and dormancy. Rc encodes a basic helix-loop-helix (bHLH) protein that was fine-mapped to an 18.5-kb region on rice chromosome 7 using a cross between Oryza rufipogon (red pericarp) and O. sativa cv Jefferson (white pericarp). Sequencing of the alleles from both mapping parents as well as from two independent genetic stocks of Rc revealed that the dominant red allele differed from the recessive white allele by a 14-bp deletion within exon 6 that knocked out the bHLH domain of the protein. A premature stop codon was identified in the second mutant stock that had a light red pericarp. RT-PCR experiments confirmed that the Rc gene was expressed in both red-and white-grained rice but that a shortened transcript was present in white varieties. Phylogenetic analysis, supported by comparative mapping in rice and maize (Zea mays), showed that Rc, a positive regulator of proanthocyanidin, is orthologous with INTENSIFIER1, a negative regulator of anthocyanin production in maize, and is not in the same clade as rice bHLH anthocyanin regulators.

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“…The partial sequence of a DFR gene from P. ovata was determined in the present study. High DFR activity is very crucial in producing high amount of anthocyanin which gives the plant the ability to quench ROS, produced due to metal toxicity.In a study on rice the correlation between higher DFR expression under ABA, excess salt stress and enhanced production of anthocyanin was established 31 .Our results showed an increase in DFRactivity and anthocyanin accumulation mainly in shoots and comparatively lower in rots treated with low Copper concentration (5 and 10 μM). It was further observed that the effect was more prominent in the shoot tissues compared to that of Root.…”

Section: Fig 3amentioning

confidence: 62%

Blood chemistry profile of Bali cattle fed silage rice straw and biosupplement of selected rumen and termites lignocellulolytic bacteria consortium

2017

JCBSC

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Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB

Cited by 127 publications

References 41 publications

Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi

Expressão gênica induzida por estresses abióticos em nódulos de feijão-caupi

Caught Red-Handed:RcEncodes a Basic Helix-Loop-Helix Protein Conditioning Red Pericarp in Rice

Blood chemistry profile of Bali cattle fed silage rice straw and biosupplement of selected rumen and termites lignocellulolytic bacteria consortium

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