Rice ERECT LEAF 1 acts in an alternative brassinosteroid signaling pathway independent of the receptor kinase OsBRI1

Sakamoto, Tomoaki; Kitano, Hidemi; Fujioka, Shozo

doi:10.1080/15592324.2017.1396404

Cited by 5 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, we reported that ELF1 is probably involved in the brassinosteroid-mediated suppression of jasmonic acid response in rice, because ELF1 suppresses stress-induced signaling and jasmonic acid signaling is stimulated in elf1-1. 24 In this study, we identified OsWRKY4 as a candidate target protein of ELF1 by yeast two-hybrid screening. ELF1 interacted with OsWRKY4 through the ARM repeats and ubiquitinated it in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…23 However, elf1-1 is not epistatic to the rice brassinosteroid receptor mutant d61, the gene expression profile of elf1-1 is distinct from that of d61 (as revealed by microarray analysis), and some stress response-related genes induced by jasmonic acid treatment are differentially regulated between elf1-1 and d61. 24 These findings imply that ELF1 suppresses stress-induced signaling and that jasmonic acid signaling is stimulated in elf1-1.…”

Section: Introductionmentioning

confidence: 91%

“…We previously reported that ELF1 acts as a positive regulator of brassinosteroid signaling, 23 and is probably involved in brassinosteroid-mediated suppression of jasmonic acid response in rice. 24 Therefore, we examined the effects of brassinosteroid (brassinolide, BL), jasmonic acid (methyl jasmonate, MeJA), or proteasome inhibitor (MG132) treatment on OsWRKY4 stability in these transgenic lines. HAT-OsWRKY4 accumulated upon MeJA or MG132 treatment in transgenic lines in the wild-type background, but the HAT-OsWRKY4 level was not changed by any treatment in the elf1-1 transgenic lines (Figure 2(b)).…”

Section: Elf1 Decreases the Stability Of Oswrky4 In Transgenic Ricementioning

confidence: 99%

See 2 more Smart Citations

ERECT LEAF1 suppresses jasmonic acid response in rice by decreasing OsWRKY4 stability

Sakamoto

Kitano

Fujioka

2018

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

ERECT LEAF 1 (ELF1), which was identified as a component of brassinosteroid signaling in rice, is involved in brassinosteroid-mediated suppression of jasmonic acid response. Here, by conducting yeast two-hybrid assay and in vitro ubiquitination experiments, we demonstrate that ELF1 interacts with the OsWRKY4 transcription factor, a positive regulator of defense responses to rice sheath blight. ELF1 decreased the stability of OsWRKY4, whereas exogenous jasmonic acid treatment suppressed this effect of ELF1, resulting in OsWRKY4 accumulation in rice plants. In wild-type rice, OsWRKY4 expression was up-regulated by jasmonic acid treatment but down-regulated by brassinosteroid treatment, suggesting that jasmonic acid-induced OsWRKY4 accumulation was caused by a combination of increased production and suppressed degradation. The expression levels of the OsWRKY4 target genes, PR1b and PR5, seemed to be correlated with the OsWRKY4 level. These results suggest that ELF1 indirectly controls the expression of PR1b and PR5 genes by regulating the OsWRKY4 protein level, and support a hypothesis that brassinosteroid and jasmonic acid cooperate to maintain the balance between growth and defense responses. We conclude that ELF1 participates in the antagonistic interaction between these two phytohormones by suppressing the jasmonic acid response through the down-regulation of OsWRKY4 protein level in rice. ARTICLE HISTORY

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Elf1 Decreases the Stability Of Oswrky4 In Transgenic Ricementioning

confidence: 99%

See 1 more Smart Citation

ERECT LEAF1 suppresses jasmonic acid response in rice by decreasing OsWRKY4 stability

Sakamoto

Kitano

Fujioka

2018

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

show abstract

PtBRI1.2 promotes shoot growth and wood formation through a brassinosteroid-mediated PtBZR1-PtWNDs module in poplar

Jiang

Song

et al. 2021

Journal of Experimental Botany

View full text Add to dashboard Cite

Brassinosteroid-insensitive-1 (BRI1) plays important roles in various signaling pathways controlling plant growth and development. However, the regulatory mechanism of BRI1 in brassinosteroid (BR)-mediated signaling for shoot growth and wood formation in woody plant is largely unknown. In this study, PtBRI1.2, a brassinosteroid-insensitive-1 gene, was overexpressed in poplar. Shoot growth and wood formation of transgenic plants were examined and the regulatory genes involved were verified. PtBRI1.2 was localized to plasma membrane, with a predominant expression in leaves. Ectopic expression of PtBRI1.2 in Arabidopsis bri1-201 and bri1-5 mutants rescued their retarded-growth phenotype. Overexpression of PtBRI1.2 in poplar promoted shoot growth and wood formation of transgenic plants. Further studies revealed that overexpression of PtBRI1.2 impelled the accumulation of PtBZR1 into the nucleus, which subsequently activated PtWNDs to up-regulate the expression of secondary cell wall biosynthesis genes involved in wood formation. Our results suggest that PtBRI1.2 plays a crucial role in regulating shoot growth and wood formation by activating BR signaling.

show abstract

Evidence of selection, adaptation and untapped diversity in Vietnamese rice landraces

Higgins

Santos²,

Khanh

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractVietnam possesses a vast diversity of rice landraces due to its geographical situation, latitudinal range, and a variety of ecosystems. This genetic diversity constitutes a highly valuable resource at a time when the highest rice production areas in the low-lying Mekong and Red River Deltas are enduring increasing threats from climate changes, particularly in rainfall and temperature patterns.We analysed 672 Vietnamese rice genomes, 616 newly sequenced, that encompass the range of rice varieties grown in the diverse ecosystems found throughout Vietnam. We described four Japonica and five Indica subpopulations within Vietnam likely adapted to the region of origin. We compared the population structure and genetic diversity of these Vietnamese rice genomes to the 3,000 genomes of Asian cultivated rice. The named Indica-5 (I5) subpopulation was expanded in Vietnam and contained lowland Indica accessions, which had with very low shared ancestry with accessions from any other subpopulation and were previously overlooked as admixtures. We scored phenotypic measurements for nineteen traits and identified 453 unique genotype-phenotype significant associations comprising twenty-one QTLs (quantitative trait loci). The strongest associations were observed for grain size traits, while weaker associations were observed for a range of characteristics, including panicle length, heading date and leaf width. We identified genomic regions selected in both Indica and Japonica subtypes during the breeding of these subpopulations within Vietnam and discuss in detail fifty-two selected regions in I5, which constitute an untapped resource of cultivated rice diversity.Our results highlight traits and their associated genomic regions, which were identified by fine phenotyping and data integration. These are a potential source of novel loci and alleles to breed a new generation of sustainable and resilient rice.

show abstract

Rice ERECT LEAF 1 acts in an alternative brassinosteroid signaling pathway independent of the receptor kinase OsBRI1

Cited by 5 publications

References 50 publications

ERECT LEAF1 suppresses jasmonic acid response in rice by decreasing OsWRKY4 stability

ERECT LEAF1 suppresses jasmonic acid response in rice by decreasing OsWRKY4 stability

PtBRI1.2 promotes shoot growth and wood formation through a brassinosteroid-mediated PtBZR1-PtWNDs module in poplar

Evidence of selection, adaptation and untapped diversity in Vietnamese rice landraces

Contact Info

Product

Resources

About