Rice Doubled Haploids and Breeding

Abstract

Cell-and tissue culture methods in combination with conventional breeding process were suitable way to produce new varieties. These applications gave new breeding alternatives to release competitive genotypes in comparison with traditional ones. To the breeding of 'Risabell' (1997) DH lines were produced via anther culture from F 2 population of a single cross combination. The new variety was improved for resistance to blast disease, high milling and cooking quality and long grain type. In case of 'Janka ' (2003) haploid cell cultures were developed and their vigorous regenerants were colchicine treated. One of the best fertile lines was released as 'Janka' has vigorous seedling growth, drought tolerance and good grain quality. The variety 'Ábel' (2005) was improved through somatic tissue culture regeneration followed by anther culture. Main characteristics of this variety are earliness, early stage cold tolerance and good performance in aerobic conditions. The breeding-processes of these State approved rice varieties demonstrate the successful integration of biotechnological methods and pedigree-breeding. A. Touraev et al. (eds.) Advances in Haploid Production in Higher Plants, 189

Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

“…Rice anther culture is a two-step process with initial callus development and subsequent green plantlet regeneration from embryogenic callus (Mishra and Rao 2016). Since the first report of anther culture, much research has aimed at optimizing the media used at each step in the process to enhance callus induction and callus regeneration (Herath et al 2010;Pauk et al 2009). This work has focused on overcoming limiting factors that reduce the efficiency of green DH plantlet production such as high genotypic dependency, low frequency of callus induction and plantlet regeneration, the low percentage of doubled haploids produced and the high ratio of albino plantlets (Lentini et al 1997).…”

Haploids: Constraints and opportunities in plant breeding

“…'Maris Haplona' rapeseed and 'Mingo' barley were the earliest releases in Canada (Ho and Jones, 1980;Thompson, 1972), while most recent releases in wheat were 'BRS 328' in Brazil and 'Emerson' in Canada (Graf et al, 2013;Scheeren et al, 2014) or 'Kharoba' in Morocco (Elhaddoury et al, 2012). In excess of 300 DH-derived cultivars, with more than 100 cultivars each in barley and rice and above 50 rapeseed cultivars were reported (Chen, 1986;Daofen, 1986;de Buyser et al, 1987;DePauw et al, 2011;Dunwell, 2010;Elhaddoury et al, 2012;Forster and Thomas, 2005;Forster et al, 2007;Graf et al, 2003Graf et al, , 2013Hu and Zeng, 1984;Humphreys et al, 2006Humphreys et al, , 2007Humphreys et al, , 2013; Jain et al, 1996;Kang et al, 2011;Loo and Xu, 1986;Palmer et al, 2005;Pauk et al, 2009;Sadasivaiah et al, 2004;Sãulescu et al, 2012;Scheeren et al, 2014;Thomas et al, 2003;Tuvesson et al, 2007;Yang and Fu, 1989;Zhao et al, 1990;Zhu and Pan, 1990). DH-derived cultivars currently occupy significant acreage in some countries.…”

Review: Improving global food security through accelerated plant breeding

“…cereal species including rice). This method has been used in rice breeding for decades [54] and like RGA, is a proven breeding method that has led to the release of many rice varieties. However due to biological factors, it has been more difficult to generate doubled haploid populations for indica subspecies compared to japonica subspecies [55].…”