Rice copine genes OsBON1 and OsBON3 function as suppressors of broad‐spectrum disease resistance

Abstract

SummaryBreeding for disease resistance is the most effective strategy to control diseases, particularly with broad‐spectrum disease resistance in many crops. However, knowledge on genes and mechanism of broad‐spectrum resistance and trade‐off between defence and growth in crops is limited. Here, we show that the rice copine genes OsBON1 and OsBON3 are critical suppressors of immunity. Both OsBON1 and OsBON3 changed their protein subcellular localization upon pathogen challenge. Knockdown of OsBON1 and dominant negative mutant of OsBON3 each enhanced resistance to rice bacterial and fungal pathogens with either hemibiotrophic or necrotrophic lifestyles. The defence activation in OsBON1 knockdown mutants was associated with reduced growth, both of which were largely suppressed under high temperature. In contrast, overexpression of OsBON1 or OsBON3 decreased disease resistance and promoted plant growth. However, neither OsBON1 nor OsBON3 could rescue the dwarf phenotype of the Arabidopsis BON1 knockout mutant, suggesting a divergence of the rice and Arabidopsis copine genes. Our study therefore shows that the rice copine genes play a negative role in regulating disease resistance and their expression level and protein location likely have a large impact on the balance between immunity and agronomic traits.

Comparative transcriptomic analysis highlights contrasting levels of resistance of Vitis vinifera and Vitis amurensis to Botrytis cinerea

“…2 and Supplemental Fig. 9 ), which are associated with enhanced resistance to B. cinerea and negative regulation of cell death, respectively 44 , 45 , is consistent with the higher resistance of the SY leaves than the RG leaves against B. cinerea . This is important, since timely recognition of pathogens and effective signaling to activate immunity are crucial for disease resistance, and any delay can impair defense responses 46 .…”

An H3K27me3 demethylase-HSFA2 regulatory loop orchestrates transgenerational thermomemory in Arabidopsis

“…SA was extracted and quantified as previously described. 51 0.1 g leaf tissues from plants syringe-infiltrated with water or Pst DC3000( avrRpt2 ) were ground for SA extraction, with four biological replicates of each genotype. Contents of SA were determined by GC-MS using a labeled internal standard.…”

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