Rice C2-Domain Proteins Are Induced and Translocated to the Plasma Membrane in Response to a Fungal Elicitor

Cy, Kim; Yd, Koo; Jb, Jin; Bc, Moon; Kang, CH; St, Kim; Park, Bo Y.; Sy, Lee; Ml, Kim; Hwang, Ilmoon; Ky, Kang; Jd, Bahk; Mj, Cho

doi:10.1021/bi034576n

Cited by 50 publications

(52 citation statements)

References 62 publications

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“…Interestingly, the CaSRC2-1 C2 domain does not contain the Wve Asp residues that are predicted to participate in Ca 2+ -dependent binding of other C2 domaincontaining proteins ( Fig. 1bc; Kim et al 2003). However, CaSRC2-1 exhibits Ca 2+ -dependent expression and under steady state conditions its expression is root-speciWc.…”

Section: Resultsmentioning

confidence: 99%

“…130 amino acid residues in length (Nalefski and Falke 1996). The C2 domain contains Wve aspartic acid residues, which form a Ca 2+ -binding site that is conserved among the Ca 2+ -dependent C2 domain-containing proteins of both plants and animals (Rizo and Süd-hof 1998;Kim et al 2003). In mammals, C2 domaincontaining proteins play roles in various cellular mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a pepper C2 domain-containing SRC2 protein implicated in resistance against host and non-host pathogens and abiotic stresses

Kim

Choi

et al. 2008

Planta

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Plants guard themselves against pathogen attack using multi-layered defense mechanism. Calcium represents an important secondary messenger during such defense responses. Upon examination of a pepper cDNA library, we observed that the gene CaSRC2-1 (Capsicum annum SRC2-1) was upregulated significantly in response to infection with the type II non-host pathogen Xanthomonas axonopodis pv. glycines 8 ra, which elicits a hypersensitive response. CaSRC2-1 encodes a protein that contains a C2 domain and it exhibits a high degree of homology to the protein Soybean genes regulated by cold 2 (SRC2). However, little is known about how SRC2 expression is elicited by biotic stresses such as pathogen challenge. Further sequence analysis indicated that the CaSRC2-1 C2 domain is unique and contain certain amino acids that are conserved within the C2 domains of other plants and animals. CaSRC2-1 transcription was up-regulated under both biotic and abiotic stress conditions, including bacterial and viral pathogen infection, CaCl(2) and cold treatment, but unaffected by treatment with plant defense-related chemicals such as salicylic acid, methyl jasmonic acid, ethephone, and abscisic acid. Intriguingly, under steady state conditions, CaSRC2-1 was expressed only in the root system. A CaSRC2-1-GFP fusion protein was used to determine localization to the plasma membrane. A fusion protein lacking the C2 domain failed to target the membrane but remained in the cytoplasm, indicating that the C2 domain plays a critical role in localization. Thus, CaSRC2-1 encodes a novel C2 domain-containing protein that targets the plasma membrane and plays a critical role in the abiotic stress and defense responses of pepper plants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a pepper C2 domain-containing SRC2 protein implicated in resistance against host and non-host pathogens and abiotic stresses

Kim

Choi

et al. 2008

Planta

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“…Several small C2 proteins in plants have been shown to be involved in abiotic and biotic stress responses. For example, rice OsERG1 is induced at the transcription level and is translocated from the cytosol to the plasma membrane by a fungal elicitor (Kim et al 2000(Kim et al , 2003. Pepper CaSRC2 responds to abiotic and biotic stresses at the transcriptional level (Kim et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a rice gene encoding a small C2 domain protein OsSMCP1 increases tolerance to abiotic and biotic stresses in transgenic Arabidopsis

Yokotani¹,

Ichikawa

Kondou

et al. 2009

Plant Mol Biol

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Plant growth and crop production are limited by environmental stress. We used a large population of transgenic Arabidopsis expressing rice full-length cDNAs to isolate the rice genes that improve the tolerance of plants to environmental stress. By sowing T2 seeds of the transgenic lines under conditions of salinity stress, the salt-tolerant line R07047 was isolated. It expressed a rice gene, OsSMCP1, which encodes a small protein with a single C2 domain, a Ca(2+)-dependent membrane-targeting domain. Retransformation of wild-type Arabidopsis revealed that OsSMCP1 is responsible for conferring the salt tolerance. It is particularly interesting that R07047 and newly constructed OsSMCP1-overexpressing Arabidopsis showed enhanced tolerance not only to high salinity but also to osmotic, dehydrative, and oxidative stresses. Furthermore, R07047 showed improved resistance to Pseudomonas syringae. The OsSMCP1 expression in rice is constitutive. Particle-bombardment-mediated transient expression analysis revealed that OsSMCP1 is targeted to plastids in rice epidermal cells. It induced overexpression of several nuclear encoded genes, including the stress-associated genes, in transgenic Arabidopsis. No marked morphological change or growth retardation was observed in R07047 or retransformants. For molecular breeding to improve the tolerance of crops against environmental stress, OsSMCP1 is a promising candidate.

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“…The EST-guided annotation of SuperSAGE tags suggested that five transporters including SS29 and SS45 were upregulated, supporting a crucial role for some transporters in plant defense. Studies have shown that various C2 domain-containing proteins (SS52) are induced by biotic and abiotic stresses (Kim et al 2003;Ouelhadj et al 2006). One of them, Arabidopsis BAP, was shown to negatively regulate defense responses (Yang et al 2006(Yang et al , 2007, but others still need to be functionally characterized.…”

Section: Functional Categories Of Supersage Tag Annotationsmentioning

confidence: 99%

SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

et al. 2008

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We used SuperSAGE, an improved version of serial analysis of gene expression, to explore transcriptome changes early in the L 3 -mediated resistance response of pepper plants against a tobamovirus. Capsicum chinense plants homozygous for the L 3 resistance gene were infected with virulent and avirulent strains of Pepper mild mottle virus (PMMoV). Plants were maintained at a temperature nonpermissive for the resistance gene to allow the viruses to spread, then transferred to a permissive temperature for 3 h and subsequently analyzed. In the incompatible reaction, we selected 152 SuperSAGE tags (each 26 nucleotides long) possibly corresponding to upregulated genes, and 84 tags for downregulated genes. Approximately 70% of tags had matching ESTs in the genus Capsicum, other genera within the Solanaceae and/or other families of plants. More than 90% of tags with EST matches could be annotated with either functionally characterized or uncharacterized proteins. We compared genes annotated by SuperSAGE tags and those annotated by partial cDNA that was obtained using the SuperSAGE tag sequences as rapid amplification of the cDNA ends-PCR primers. Of genes annotated by SuperSAGE tags, c. 90% were consistent with those annotated by longer cDNA sequences. We cloned 17 full-length cDNAs from different SuperSAGE tags and confirmed that these genes were upregulated during normal infection in the incompatible interaction. We identified several early resistance response genes including a Ran/TC4 protein and a b-oxidation multifunctional protein, indicating that SuperSAGE is a powerful tool for investigating plant-pathogen interactions.

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Rice C2-Domain Proteins Are Induced and Translocated to the Plasma Membrane in Response to a Fungal Elicitor

Cited by 50 publications

References 62 publications

Molecular characterization of a pepper C2 domain-containing SRC2 protein implicated in resistance against host and non-host pathogens and abiotic stresses

Molecular characterization of a pepper C2 domain-containing SRC2 protein implicated in resistance against host and non-host pathogens and abiotic stresses

Overexpression of a rice gene encoding a small C2 domain protein OsSMCP1 increases tolerance to abiotic and biotic stresses in transgenic Arabidopsis

SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

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