Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling

Abstract

This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. OPEN ACCESS Molecules 2014, 19 5326FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-B activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination.

The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

“…In recent studies, we [5] and others [42] have demonstrated that dietary fibers can signal and modulate immune responses via binding to PRRs [5,43,44]. Our data suggest that TLR receptors are also involved in the effects of the currently tested fibers as the immunomodulating capacity was reduced in the presence of a MyD88 inhibitor [5,30,44].…”

Identification and activation of TLR4-mediated signalling pathways by alginate-derived guluronate oligosaccharide in RAW264.7 macrophages

“…An increasing number of studies in recent years have proven that various oligosaccharides, including chitosan oligosaccharide, alginate oligosaccharide and rice bran feruloylated oligosaccharide, regulate immune functions via the MAPK pathway 30–32 . Our previous results showed that GOS could activate all MAPKs, including p38, JNK and ERK 19 .…”

In Vitro Evidence for Immune-Modulatory Properties of Non-Digestible Oligosaccharides: Direct Effect on Human Monocyte Derived Dendritic Cells

“…Several studies describe an immune modulation by different NDO on cellular level via the interaction with specific sugar receptors including DC-SIGN, TLRs or C type lectin receptors [ 24 , 25 , 33 – 35 ]. Further studies in our experimental setting are necessary to investigate the role of other types of receptors involved in scGOS/lcFOS-mediated, direct mechanisms on human MoDC.…”