Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Giri, Jitender; Bhosale, Rahul; Huang, Guoqiang; Pandey, Bharati; Parker, Hélène; Zappala, Susan; Yang, Jinhua; Diévart, Anne; Bureau, Charlotte; Ljung, Karin; Price, Adam H.; Rose, Terry J.; Larrieu, Antoine; Mairhofer, Stefan; Sturrock, Craig J.; White, Philip J.; Dupuy, Lionel; Hawkesford, Malcolm J.; Périn, Christophe; Liang, Wanqi; Péret, Benjamin; Hodgman, Charlie; Lynch, Jonathan P.; Wissuwa, Mathieu; Zhang, Dabing; Pridmore, Tony; Mooney, Sacha J.; Guiderdoni, Emmanuel; Swarup, Ranjan; Bennett, Malcolm J.

doi:10.1038/s41467-018-03850-4

Cited by 119 publications

(101 citation statements)

References 35 publications

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“…Differences in PIN expression between trichoblast and atrichoblast cells lead to different auxin gradients between cell files and contribute to the site determination of root hair initiation (Balcerowicz et al, 2015). Likewise, AUX1 contributes to this auxin gradient and promotes root hair elongation in Arabidopsis and rice Giri et al, 2018…”

Section: Root Hair Developmentmentioning

confidence: 99%

Tackling Plant Phosphate Starvation by the Roots

2019

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Plant responses to phosphate deprivation encompass a wide range of strategies, varying from altering root system architecture, entering symbiotic interactions to excreting root exudates for phosphorous release, and recycling of internal phosphate. These processes are tightly controlled by a complex network of proteins that are specifically upregulated upon phosphate starvation. Although the different effects of phosphate starvation have been intensely studied, the full extent of its contribution to altered root system architecture remains unclear. In this review, we focus on the effect of phosphate starvation on the developmental processes that shape the plant root system and their underlying molecular pathways.

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Section: Root Hair Developmentmentioning

confidence: 99%

Tackling Plant Phosphate Starvation by the Roots

2019

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“…We used these conditions as we found that osmotic stress on its own often leads to an increased percentage of root hairs that cease growing shortly after initiation, which strongly decreases the overall root hair length (Fig. 4), and because low Pi conditions are known to increase root hair formation of Arabidopsis and rice when grown in vitro (Bates and Lynch, 1996; Bhosale et al, 2018; Giri et al, 2018). We first assessed root hairs under low Pi only, so we were able to interpret the combined treatment.…”

Section: Resultsmentioning

confidence: 99%

Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)

McKay

McFarlane

et al. 2020

Preprint

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31The secretary and endocytic pathways intersect at the trans-Golgi network/Early Endosome 32 (TGN/EE). TGN/EE function depends on a careful balance of ions within this compartment, 33 and the electrochemical potential across its membrane. The identity of the proton pump 34 required for acidification and the transporters that catalyse cation and anion import into 35 endosome compartments are known. However, the protein needed to complete the transport 36 circuit, and that mediates cation and anion efflux from the TGN/EE has not been identified. 37Here, we characterize Cation Chloride Cotransporters (CCC1s) from Arabidopsis and rice. 38 We find that the AtCCC1 is localized to the TGN/EE, where it modulates important TGN 39 functions such as endocytosis, exocytosis, and cell wall synthesis/secretion. Loss of CCC1 40 results in severe, widespread phenotypes in both rice and Arabidopsis, including plant growth 41 and developmental perturbations, defects in root hair elongation and altered osmoregulation, 42 consistent with CCC performing a core cellular function. Complementation of the root hair 43 elongation phenotype of Atccc1 with root hair specific expression of GFP-AtCCC1 44 demonstrates CCC1 functions within the TGN/EE. Collectively, our results imply that CCC1 45 is a strong candidate for the missing component of the TGN/EE ion transportcircuit. 46 47 126 7including root hairs, and in the gynoecium, while GUS staining did not indicate expression in 127 these cells. This is likely due to the different sensitivities of the two methods. 128 129 ccc1 knockout mutants have a reduced root hair length and tip growth rate 130 As our expression analysis showed that AtCCC1 is expressed in root hairs (Fig. 1), we 131 examined whether root hair cells of the knockouts had an altered phenotype, and whether we 132 could use this model cell type to investigate the role of CCC1. Characterisation of the 133 knockouts showed that both Atccc1 and Osccc1.1 plants had a reduction in the overall length 134 of their root hairs compared to control plants (Fig. 2); and a complete lack of collet hairs in 135 Atccc1. Collet hairs are epidermal root hairs formed in some plant species in the transition 136 zone between the root and the hypocotyl (Fig. 2, Sliwinska et al., 2015). Atccc1 also had 137 branching and bulging of root hairs, although, at a low frequency, and root hairs of the 138 495 496 References 497 Balcerowicz, D., Schoenaers, S., and Vissenberg, K. (2015). Cell fate determination and 498 the switch from diffuse growth to planar polarity in Arabidopsis root epidermal cells. 499 Frontiers in Plant Science 6, 1163. 500 Bassil, E., and Blumwald, E. (2014). The ins and outs of intracellular ion homeostasis: 501 NHX-type cation/H + transporters. Current Opinion in Plant Biology 22, 1-6. 502 23 Bassil, E., Ohto, M.-a., Esumi, T., Tajima, H., Zhu, Z., Cagnac, O., Belmonte, M., Peleg, 503 Z., Yamaguchi, T., and Blumwald, E. (2011). The Arabidopsis intracellular Na + /H + 504 antiporters NHX5 and NHX6 are endosome associated ...

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“…To harden the X-ray beam, a 1.0 mm Cu filter was used. Recent studies on rice roots that involved X-ray CT have been listed in Table 2 [40,41,42,43,44,45,46]. Review of these studies indicated that most studies used pots with diameter < 100 mm and required scanning times of > one h. This indicated that our developed process flow is suitable for 4-D RSA phenotyping, when applied to rice showing relatively large RSAs.…”

Section: Discussionmentioning

confidence: 99%

High-throughput three-dimensional visualization of root system architecture of rice using X-ray computed tomography

Teramoto¹,

Takayasu²,

Kitomi³

et al. 2020

Preprint

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Background: X-ray computed tomography (CT) allows us to visualize root system architecture (RSA) beneath the soil, non-destructively and in a three-dimensional (3-D) form. However, CT scanning, reconstruction processes, and root isolation from X-ray CT volumes, take considerable time. For genetic analyses, such as quantitative trait locus mapping, which require a large population size, a high-throughput RSA visualization method is required.Results: We have developed a high-throughput process flow for the 3-D visualization of rice (Oryza sativa) RSA (consisting of radicle and crown roots), using X-ray CT. The process flow includes use of a uniform particle size, calcined clay to reduce the possibility of visualizing non-root segments, use of a higher tube voltage and current in the X-ray CT scanning to increase root-to-soil contrast, and use of a 3-D median filter and edge detection algorithm to isolate root segments. Using high-performance computing technology, this analysis flow requires only 10 min (33 s, if a rough image is acceptable) for CT scanning and reconstruction, and 2 min for image processing, to visualize rice RSA. This reduced time allowed us to conduct the genetic analysis associated with 3-D RSA phenotyping. In 2-week-old seedlings, 85% and 100% of radicle and crown roots were detected, when 16 cm and 20 cm diameter pots were used, respectively. The X-ray dose per scan was estimated at < 0.09 Gy, which did not impede rice growth. Using the developed process flow, we were able to follow daily RSA development, i.e., 4-D RSA development, of an upland rice variety, over three weeks. Conclusions: We developed a high-throughput process flow for 3-D rice RSA visualization by X-ray CT. The X-ray dose assay on plant growth has shown that this methodology could be applicable for 4-D RSA phenotyping. We named the RSA visualization method ‘RSAvis3D’ and are confident that it represents a potentially efficient application for 3-D RSA phenotyping of various plant species.

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Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Cited by 119 publications

References 35 publications

Tackling Plant Phosphate Starvation by the Roots

Tackling Plant Phosphate Starvation by the Roots

Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)

High-throughput three-dimensional visualization of root system architecture of rice using X-ray computed tomography

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