Rice arbuscular mycorrhiza as a tool to study the molecular mechanisms of fungal symbiosis and a potential target to increase productivity

Nakagawa, Tomomi; Imaizumi-Anraku, Haruko

doi:10.1186/s12284-015-0067-0

Cited by 20 publications

(11 citation statements)

References 78 publications

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“…Glomus was the most frequently encountered AMF phylotype in all regions, except for the Ashanti region. The predominance of Glomus phylotypes in rice plants was revealed by several studies, irrespective of the conventional (wet paddy) system or production under rain-fed conditions [49][50][51]62]. The consistent occurrence of Glomus in these varying conditions of production indicated that the genus Glomus could withstand several environmental conditions and was adapted to various ecosystems [40,61].…”

Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Associated with Rice (Oryza sativa L.) in Ghana: Effect of Regional Locations and Soil Factors on Diversity and Community Assembly

et al. 2020

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Understanding the community composition and diversity of arbuscular mycorrhizal fungi (AMF) in an agricultural ecosystem is important for exploiting their potential in sustainable crop production. In this study, we described the genetic diversity and community structure of indigenous AMF in rain-fed rice cultivars across six different regions in Ghana. The morphological and molecular analyses revealed a total of 15 different AMF genera isolated from rice roots. Rhizophagus and Glomus were observed to be predominant in all regions except the Ashanti region, which was dominated by the genera Scutellospora and Acaulospora. A comparison of AMF diversity among the agroecological zones revealed that Guinea Savannah had the highest diversity. Permutational Multivariate Analysis of Variance (PERMANOVA) analysis indicated that the available phosphorus (AP) in the soil was the principal determining factor for shaping the AMF community structure (p < 0.05). We report, for the first time, AMF diversity and community structure in rice roots and how communities are affected by the chemical properties of soil from different locations in Ghana.

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Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Associated with Rice (Oryza sativa L.) in Ghana: Effect of Regional Locations and Soil Factors on Diversity and Community Assembly

et al. 2020

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“…2 ; 37, 47, 54, 59). Non-leguminous plants have mutualistic symbiotic relationships with arbuscular mycorrhizal fungi through the CSP ( 37 , 42 , 55 ). Ca 2+ /calmodulin–dependent protein kinase (encoded by CCaMK ) has been identified as a key component of the CSP, which is required for rhizobial and mycorrhizal endosymbioses to take up N and phosphorus, respectively ( 37 , 47 , 54 – 56 , 59 ).…”

Section: Plant Genes For Microbial Symbiosesmentioning

confidence: 99%

Are Symbiotic Methanotrophs Key Microbes for N Acquisition in Paddy Rice Root?

et al. 2016

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The relationships between biogeochemical processes and microbial functions in rice (Oryza sativa) paddies have been the focus of a large number of studies. A mechanistic understanding of methane–nitrogen (CH4–N) cycle interactions is a key unresolved issue in research on rice paddies. This minireview is an opinion paper for highlighting the mechanisms underlying the interactions between biogeochemical processes and plant-associated microbes based on recent metagenomic, metaproteomic, and isotope analyses. A rice symbiotic gene, relevant to rhizobial nodulation and mycorrhization in plants, likely accommodates diazotrophic methanotrophs or the associated bacterial community in root tissues under low-N fertilizer management, which may permit rice plants to acquire N via N2 fixation. The amount of N fixed in rice roots was previously estimated to be approximately 12% of plant N based on measurements of 15N natural abundance in a paddy field experiment. Community analyses also indicate that methanotroph populations in rice roots are susceptible to environmental conditions such as the microclimate of rice paddies. Therefore, CH4 oxidation by methanotrophs is a driving force in shaping bacterial communities in rice roots grown in CH4-rich environments. Based on these findings, we propose a hypothesis with unanswered questions to describe the interplay between rice plants, root microbiomes, and their biogeochemical functions (CH4 oxidation and N2 fixation).

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“…We also developed a Falcon-wick hydroponics system for larger AM model plants. We choose the important crop and model plant rice, because it currently represents the prime monocots model for studying molecular mechanisms of mycorrhizal colonization (Nakagawa and Imaizumi-Anraku, 2015). We grew rice in a bigger hydroponics system using Falcon tubes instead of pipette tips as a support (Falcon-wick system, Figure 5).…”

Section: Falcon-wick Hydroponics For Colonization Of Rice Roots By Armentioning

confidence: 99%

A Flexible, Low-Cost Hydroponic Co-Cultivation System for Studying Arbuscular Mycorrhiza Symbiosis

et al. 2020

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Arbuscular mycorrhiza (AM) is a widespread symbiosis between plant roots and fungi of the Glomeromycotina, which improves nutrient uptake by plants. The molecular mechanisms underlying development and function of the symbiosis are subject to increasing research activity. Since AM occurs in the soil, most studies targeting a molecular understanding of AM development and function, use solid substrates for cocultivating plants and AM fungi. However, for some experiments very clean roots, highly controlled nutrient conditions or applications of defined concentrations of signaling molecules (such as hormones) or other small chemicals (such as synthetic inhibitors or signaling agonists) are needed. To this end, hydroponics has been widely used in research on mechanisms of plant nutrition and some hydroponic systems were developed for AM fungal spore amplification. Here, we present a hydroponics setup , which can be successfully utilized for experimental root colonization assays. We established a "tipwick" system based on pipette tips and rock wool wicks for co-cultivation of AM fungi with small model plants such as Lotus japonicus. A larger "Falcon-wick" system using Falcon tubes and rockwool wicks was developed for larger model plants such as rice. The hydroponic system can also be employed for growing L. japonicus hairy roots after transformation by Agrobacterium rhizogenes, thus circumventing the laborious cultivation on agar medium-containing Petri dishes during hairy root development. The tip-wick and Falcon-wick systems are easy to use and can be built with low cost, conventional and reusable lab plastic ware and a simple aquarium pump.

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Rice arbuscular mycorrhiza as a tool to study the molecular mechanisms of fungal symbiosis and a potential target to increase productivity

Cited by 20 publications

References 78 publications

Arbuscular Mycorrhizal Fungi Associated with Rice (Oryza sativa L.) in Ghana: Effect of Regional Locations and Soil Factors on Diversity and Community Assembly

Arbuscular Mycorrhizal Fungi Associated with Rice (Oryza sativa L.) in Ghana: Effect of Regional Locations and Soil Factors on Diversity and Community Assembly

Are Symbiotic Methanotrophs Key Microbes for N Acquisition in Paddy Rice Root?

A Flexible, Low-Cost Hydroponic Co-Cultivation System for Studying Arbuscular Mycorrhiza Symbiosis

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