Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants

Sathish, P.; Gamborg, O. L.; Nabors, Murray W.

doi:10.1007/bf00234049

Cited by 21 publications

(8 citation statements)

References 14 publications

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“…Therefore, at present various tissue culture techniques are being used for the genetic improvement of rice plant throughout the world (Raina, 1989). Among techniques, anther culture, protoplast fusion and culture, leaf culture, root culture, immature embryo culture and mature seed culture are important in rice to create additional variation and novel rice varieties (Lutts et al, 2001;Sathish et al, 1995). It has been demonstrated in several cases that when higher plant tissues undergo a process of dedifferentiation and cell proliferation in vitro, wide range of mutations occur at a frequency much higher than expected (Brar and Khush, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Genotype and Culture Media on Callus Induction and Plant Regeneration from Matured Rice Grain Culture

Niroula

Sah

Bimb

et al. 2005

J. Inst. Agric. Anim. Sci.

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This study was under taken to elucidate the effect of genotypes and media compositions on callus induction from mature rice seeds. Three different callus induction media, designated as A (N6 mineral salts + N6 vitamins, 2 mg/l each + myoinositol, 100 mg/l + 2,4-D, 2.5 mg/l + kinetin, 0.5mg/l + AgNO 3 , 10 mg/l + maltose, 50 gm/l); B (MS organic salts + N6 mineral salts + NAA, 4 mg/l + kinetin, 2 mg/l + AgNO 3 , 5mg/l and sucrose, 60 gm/l); and C (B media without AgNO 3 ), and six rice genotypes viz. Jumlimarshi, Tilki, Jethobudo, Manshara, Masuli and Pahenle were evaluated. The modified N6 medium supplemented with 2, 4-D, 2.5 mg/l and AgNO 3 , 10 mg/l exhibited better performance in callus induction. Among genotypes, callus induction frequency was higher (100%) in Masuli, Tilki and Jumlimarshi regardless of media tested. The positive effect of AgNO 3 was only observed in medium A for quality callus induction and subsequent plant regeneration. The genotype Tilki performed better regarding plant regeneration (27.77%). Therefore, it is suggested that application of medium A is advantageous to accomplish overall efficiency of callus induction and plant regeneration from seeds of various rice genotypes.

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Section: Introductionmentioning

confidence: 99%

“…Several high yielding rice varieties were developed through the application of anther culture, and other forms of somaclones in rice (Lutts et al, 2001, Gupta, 1999, Sathish et al, 1995, Raina, 1989. However, plant regeneration from callus obtained from somatic tissue is more successful than that of anther culture (Guo and Cao, 1982).…”

Section: Introductionmentioning

confidence: 99%

Effect of Genotype and Culture Media on Callus Induction and Plant Regeneration from Matured Rice Grain Culture

Niroula

Sah

Bimb

et al. 2005

J. Inst. Agric. Anim. Sci.

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“…Somaclonal variation was also frequently recognized in anther culture (Sathish et al 1995, Sugimoto et al 1999. Methylation of deoxyribonucleic acid may be involved in somaclonal variation.…”

Section: Introductionmentioning

confidence: 99%

Stability of Characters of a Doubled Haploid Rice Variety, Shirayukihime.

Sugimoto

Arai

2002

Breed. Sci.

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In order to determine whether anther-derived varieties and conventional varieties behave in the same manner as theoretically assumed, the stability of the characters in a doubled haploid variety Shirayukihime, developed by anther culture of rice (Oryza sativa L.), was evaluated compared with three varieties, Koshihikari, Nipponbare and Hatsushimo, developed by conventional methods of breeding. Days to heading, culm length, panicle length and panicle number of those varieties were measured or counted generation by generation for ten years. The days from the beginning to the end of heading were not different between the doubled haploid variety and the conventional varieties. The stability of culm length and panicle length was not different among the four varieties based on the non-significant difference in their coefficients of variation (CV). The CV in culm length was found to be highly stable, as no significance was detected for year-to-year and variety-to-variety. In the CV of panicle numbers, significant differences were detected in both years and varieties, between early and late plantings. However, no significant difference was detected between the doubled haploid variety and the conventional varieties in both plantings. Therefore, the CV in the characters of Shirayukihime were not higher than those of the other varieties. These results suggest that the stability of the characters of the doubled haploid variety might not be different from those of conventional varieties. The conventional method of breeding could thus be efficiently supplemented with the anther culture method.

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“…Untuk membuktikan terjadi atau tidaknya variasi gametoklonal dapat dilakukan dengan membandingkan populasi DH dengan populasi F 8 SSD yang merupakan populasi yang identik dengan populasi haploid ganda. Jika tidak muncul, maka karakter tersebut merupakan variasi gametoklonal (Moon et al 1996, Zhuravlev dan Zmeeva 1996, Satish et al 1995.…”

Section: Hasil Dan Pembahasan Karakter Morfologiunclassified

Karakterisasi Galur Haploid Ganda Hasil Kultur Antera Padi

Dewi

Trilaksana

Koesoemaningtyas

et al. 2016

BPN

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New genetic variation in rice can be obtained by crossing between subspecies of rice (Indica, Japonica, and Javanica). Variability is a must in breeding materials. The objectives of this research were to evaluate morphological and agronomical characters of doubled-haploid lines derived from anther culture of F 1 and to determine maternal effect from reciprocal crosses. The materials used in the experiment were 144 haploid lines derived from anther culture of F 1 obtained from reciprocal crossing between Asemandi/Taipei 309 and Taipei 309/ Asemandi. Asemandi belongs to O. sativa subspecies Javanica, whilst Taipei 309 is subspecies Japonica. Evaluation on morphological and agronomical characters was based on Standard Evaluation System for Rice from IRRI. The results indicated that agronomical characters between haploid lines were varied, but individuals in lines were similar as shown by all morphological and agronomical characters. New characters were also found in leaf and stem angle, awn existence, awn length, awn color, panicle type and exertion, axis type, secondary branch of panicle, and grain type. Maternal effect could be seen only in booting stage, number of sterile tillers and percentage of fertile tillers. Based on number of fertile tillers, number of fertile spikelets, fertility, weight of 100 grains and yield/plant,

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Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants

Cited by 21 publications

References 14 publications

Effect of Genotype and Culture Media on Callus Induction and Plant Regeneration from Matured Rice Grain Culture

Effect of Genotype and Culture Media on Callus Induction and Plant Regeneration from Matured Rice Grain Culture

Stability of Characters of a Doubled Haploid Rice Variety, Shirayukihime.

Karakterisasi Galur Haploid Ganda Hasil Kultur Antera Padi

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