Rice aleurone layer specific OsNF-YB1 regulates grain filling and endosperm development by interacting with an ERF transcription factor

Xu, Jingjing; Zhang, Xiaofan; Hou, Xue

doi:10.1093/jxb/erw409

Cited by 99 publications

(115 citation statements)

References 42 publications

(65 reference statements)

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…mRNA in situ hybridization result showed that NF‐YB1 majorly accumulated in embryo and alueron layer, but not in the endosperm of the 7 DAP seeds (Figure c). As shown in Figure S1a‐c, NF‐YB1 protein accumulated in nuclear and cytoplasm, which is consistent with the previous reports (E et al ., ; Xu et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…During the process of our NF‐YB1 project, at least four independent labs reported their works on NF‐YB1 with similar biological functions (Bai et al ., ; E et al ., ; Sun et al ., ; Xu et al ., ). In consistence with this study, RNAi or CRISPR/Cas9 knock‐out lines of NF‐YB1 had smaller seeds and chalky endosperms, when compared with the WT.…”

Section: Discussionmentioning

confidence: 97%

“…() emphasized the key role of NF‐YB1 in modulating the expression of sucrose transporters in aleurone to enhance sugar loading to the endosperm (Bai et al ., ). As described in a very recent publication, by forming a heterotrimer complex with NF‐YC and OsERF115, NF‐YB1 promote the binding of OsERF115 to GCC box of the downstream gene promoters, hence to regulate rice grain filling and endosperm development (Xu et al ., ). In this study, we revealed that NF‐YB1 regulates various aspects of grain quality and nutrient synthesis, in addition to the grain size and chalkiness.…”

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Bello

Hou

Zhao

et al. 2019

Plant Biotechnology Journal

107

View full text Add to dashboard Cite

Summary Identification of seed development regulatory genes is the key for the genetic improvement in rice grain quality. NF ‐Ys are the important transcription factors, but their roles in rice grain quality control and the underlying molecular mechanism remain largely unknown. Here, we report the functional characterization a rice NF ‐Y heterotrimer complex NF ‐ YB 1‐ YC 12‐ bHLH 144, which is formed by the binding of NF ‐ YB 1 to NF ‐ YC 12 and then bHLH 144 in a sequential order. Knock‐out of each of the complex genes resulted in alteration of grain qualities in all the mutants as well as reduced grain size in crnf‐yb1 and crnf‐yc12 . RNA ‐seq analysis identified 1496 genes that were commonly regulated by NF ‐ YB 1 and NF ‐ YC 12 , including the key granule‐bound starch synthase gene Wx . NF ‐ YC 12 and bHLH 144 maintain NF ‐ YB 1 stability from the degradation mediated by ubiquitin/26S proteasome, while NF ‐ YB 1 directly binds to the ‘G‐box’ domain of Wx promoter and activates Wx transcription, hence to regulate rice grain quality. Finally, we revealed a novel grain quality regulatory pathway controlled by NF ‐ YB 1‐ YC 12‐ bHLH 144 complex, which has great potential for rice genetic improvement.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

Bello

Hou

Zhao

et al. 2019

Plant Biotechnology Journal

107

View full text Add to dashboard Cite

show abstract

“…In mammals, genome-wide association with selected transcription factors indicated that the NF-Y complex directly binds to the CCAAT box and associates with other cis -elements by interacting with different transcription factors (Dolfini et al, 2016; Zambelli and Pavesi, 2016). Recently, a ChIP-sequencing approach in rice identified genome-wide downstream targets of OsNF-YB1, indicating that OsNF-YB1 directly binds to the CCAAT box and associates with the GCC box by interacting with an ERF-type transcription factor (Xu J.J. et al, 2016). …”

Section: Main Textmentioning

confidence: 99%

The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

et al. 2017

View full text Add to dashboard Cite

Nuclear factor Y (NF-Y) is an evolutionarily conserved trimeric transcription factor complex present in nearly all eukaryotes. The heterotrimeric NF-Y complex consists of three subunits, NF-YA, NF-YB, and NF-YC, and binds to the CCAAT box in the promoter regions of its target genes to regulate their expression. Yeast and mammal genomes generally have single genes with multiple splicing isoforms that encode each NF-Y subunit. By contrast, plant genomes generally have multi-gene families encoding each subunit and these genes are differentially expressed in various tissues or stages. Therefore, different subunit combinations can lead to a wide variety of NF-Y complexes in various tissues, stages, and growth conditions, indicating the potentially diverse functions of this complex in plants. Indeed, many recent studies have proved that the NF-Y complex plays multiple essential roles in plant growth, development, and stress responses. In this review, we highlight recent progress on NF-Y in Arabidopsis thaliana, including NF-Y protein structure, heterotrimeric complex formation, and the molecular mechanism by which NF-Y regulates downstream target gene expression. We then focus on its biological functions and underlying molecular mechanisms. Finally, possible directions for future research on NF-Y are also presented.

show abstract

“…When the expression of MxIRT and MxHA2 rose, the active iron content and efflux in M. xiaojinensis roots were increased significantly [21]. The interaction of OsERF115 with OsNF-YB1 in Oryza sativa was confirmed and OsNF-YB1 specifically regulated the transcription of downstream genes during rice endosperm development by forming protein complexes consisting of OsNF-YB1, OsNF-YC and ERF [22]. CitVHA-c4, a kind of V-ATPase in citrus, was observed to interact with CitERF13 using a yeast twohybrid screen and BiFC.…”

Section: Introductionmentioning

confidence: 99%

Screening of proteins interacting with ERF transcriptional factor from Populus simonii × P.nigra by yeast two-hybrid method

Wang

Xia

Jiang

2018

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

To investigate the biology functions of ERF transcriptional factor in Populus simonii £ P.nigra, the proteins interacting with ERF were screened by the yeast two-hybrid method. The bait vector harbouring ERF and cDNA library of Populus simonii £ P.nigra were constructed. The bait had no self-activation and toxicity to yeast cells in the yeast two-hybrid system. The library titering experiment indicated that it could meet the requirement for the yeast two-hybrid library screen. Five proteins that interacted with ERF were obtained, including galactinol-sucrose galactosyl transferase 2, phosphoinositide-specific phospholipase C family protein, MLP-like protein, senescence-associated protein and an unknown protein. Real-time polymerase chain reaction showed that the expression levels of ERF and five interacting protein genes increased after salt treatment. Gene ontology analysis associated most of the interacting proteins with stress response. These results showed that ERF might share similar function with these proteins and they act together to regulate the salt response of Populus simonii £ P.nigra. This study provided experimental evidence for further revealing the biological function of the ERF transcription factor in poplar.

show abstract

Rice aleurone layer specific OsNF-YB1 regulates grain filling and endosperm development by interacting with an ERF transcription factor

Abstract: HighlightRice transcription factor OsNF-YB1 is specifically expressed in the aleurone layer of developing endosperm and forms protein complexes consisting of OsNF-YB1, OsNF-YC and ERF to regulate grain filling and endosperm development.

Cited by 99 publications

References 42 publications

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

NF‐YB1‐YC12‐bHLH144 complex directly activates Wx to regulate grain quality in rice (Oryza sativa L.)

The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

Screening of proteins interacting with ERF transcriptional factor from Populus simonii × P.nigra by yeast two-hybrid method

Contact Info

Product

Resources

About