RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice

Abstract

Backgrounds Acyl-coenzyme A (CoA) esters are important intermediates in lipid metabolism with regulatory properties. Acyl-CoA-binding proteins bind and transport acyl-CoAs to fulfill these functions. RICE ACYL-COA-BINDING PROTEIN6 (OsACBP6) is currently the only one peroxisome-localized plant ACBP that has been proposed to be involved in β-oxidation in transgenic Arabidopsis. The role of the peroxisomal ACBP (OsACBP6) in rice (Oryza sativa) was investigated. Results Here, we report on the function of OsACBP6 in rice. The osacbp6 mutant showed diminished growth with reduction in root meristem activity and leaf growth. Acyl-CoA profiling and lipidomic analysis revealed an increase in acyl-CoA content and a slight triacylglycerol accumulation caused by the loss of OsACBP6. Comparative transcriptomic analysis discerned the biological processes arising from the loss of OsACBP6. Reduced response to oxidative stress was represented by a decline in gene expression of a group of peroxidases and peroxidase activities. An elevation in hydrogen peroxide was observed in both roots and shoots/leaves of osacbp6. Taken together, loss of OsACBP6 not only resulted in a disruption of the acyl-CoA homeostasis but also peroxidase-dependent reactive oxygen species (ROS) homeostasis. In contrast, osacbp6-complemented transgenic rice displayed similar phenotype to the wild type rice, supporting a role for OsACBP6 in the maintenance of the acyl-CoA pool and ROS homeostasis. Furthermore, quantification of plant hormones supported the findings observed in the transcriptome and an increase in jasmonic acid level occurred in osacbp6. Conclusions In summary, OsACBP6 appears to be required for the efficient utilization of acyl-CoAs. Disruption of OsACBP6 compromises growth and led to provoked defense response, suggesting a correlation of enhanced acyl-CoAs content with defense responses.

Genetic Analysis and Fine Mapping of QTL for the Erect Leaf in Mutant mths29 Induced through Fast Neutron in Wheat

“…Currently, DGAT3 stands as the sole identified 2Fe-2S protein implicated in the biosynthesis of triacylglycerol (TAG) in plants [ 36 ]. The content of TAG influences root meristem activity and leaf growth and development [ 37 ]. Another down-regulated gene, CG23 , encodes a protein containing the R3H domain, which modulates RNA binding ability and catalytic function [ 38 ].…”

Machine Vision Approach for Classification of Rice Varieties Using Texture Features

“…Humans need food and nutrition for good health and living purpose. Metabolism development of rice seeds is most important for increase food yield [12] , and in [13] , Leaf and roots growth are shown better performance for paddy crop yield in agriculture. Acyl-Coenzyme-A (CoA) protein and transport acyl-CoAs bind was performed better metabolism properties in leaf and roots activities.…”

Overexpression of rice acyl-CoA-binding protein OsACBP5 protects Brassica napus against seedling infection by fungal phytopathogens

“…In contrast, the overexpression of a homologous Class III ACBP from Vitis vinifera (grape) in Arabidopsis displayed enhanced resistance to P. syringae and a hemibiotrophic fungal pathogen Colletotrichum higginsianum (Takato et al 2013). ACBPs from monocotyledonous plants such as rice are less well studied compared to ACBPs from dicotyledonous plants (Xiao and Chye 2011b;Xia et al 2012;Takato et al 2013); nevertheless, there have been reports on the function of the rice ACBPs (OsACBPs) (Meng et al 2011(Meng et al , 2020Meng and Chye 2014;Du et al 2016;Guo et al 2017Guo et al , 2019Panthapulakkal Narayanan et al 2019Liao et al 2020;Guo et al 2021). OsACBP5, the sole rice Class III ACBP, resembles AtACBP3 in the location of the acyl-CoA-binding (ACB) domain at the carboxyl (C-)terminus, unlike the other three ACBP classes (Meng et al 2011).…”

“…2013); nevertheless, there have been reports on the function of the rice ACBPs (OsACBPs) (Meng et al . 2011, 2020; Meng and Chye 2014; Du et al . 2016; Guo et al .…”