Ribulose-1,5-bisphosphate carboxylase/oxygenase from an ammonia-oxidizing bacterium, Nitrosomonas sp. K1: Purification and properties

Hatayama, Ryota; Takahashi, Reiji; Ohshima, Mifuyu; SHIBASAKI, RIE; Tokuyama, Tatsuaki

doi:10.1016/s1389-1723(01)80013-x

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…Given that the ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO, green form, EC 4.1.1.39) activity in M. capsulatus (Bath) when grown on methane (Taylor et al ., ; Stanley & Dalton, ), some of the CO 2 produced could be reassimilated, but this is not the case when Hg 2+ and Hg are present, which would indicate that one of these species inhibits RuBisCO activity, as is the case in Nitrosomonas sp. K1 (Hatayama et al ., ). Detoxification of Hg 2+ by reduction to elemental mercury would require methane–carbon to be oxidized to CO 2 and for the reducing equivalents formed to be prioritized to the detoxification system.…”

Section: Resultsmentioning

confidence: 97%

Response to mercury (II) ions in Methylococcus capsulatus (Bath)

Boden

Murrell

2011

FEMS Microbiol Lett

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The mercury (II) ion is toxic and is usually detoxified in Bacteria by reduction to elemental mercury, which is less toxic. This is catalysed by an NAD(P)H-dependent mercuric reductase (EC 1.16.1.1). Here, we present strong evidence that Methylococcus capsulatus (Bath) - a methanotrophic member of the Gammaproteobacteria - uses this enzyme to detoxify mercury. In radiorespirometry studies, it was found that cells exposed to mercury dissimilated 100% of [(14) C]-methane provided to generate reducing equivalents to fuel mercury (II) reduction, rather than the mix of assimilation and dissimilation found in control incubations. The detoxification system is constitutively expressed with a specific activity of 352 (±18) nmol NADH oxidized min(-1) (mg protein)(-1) . Putative mercuric reductase genes were predicted in the M. capsulatus (Bath) genome and found in mRNA microarray studies. The MerA-derived polypeptide showed high identity (> 80%) with MerA sequences from the Betaproteobacteria.

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Section: Resultsmentioning

confidence: 97%

Response to mercury (II) ions in Methylococcus capsulatus (Bath)

Boden

Murrell

2011

FEMS Microbiol Lett

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“…Similar observations were made by Antoniou et al [45], who report the overall nitrification pH of approximately 7.8. There are three major bacteria, for which optimal pH conditions are as follows: (1) Nitrobacter: 7.5 [46]; (2) Nitrosomonas: 7.0-7.5 [47], and (3) Nitrospira: 8.0-8.3 [48].…”

Section: Ph Stabilizationmentioning

confidence: 99%

Challenges of Sustainable and Commercial Aquaponics

et al. 2015

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Abstract:The world is facing a number of serious problems of which population rise, climate change, soil degradation, water scarcity and food security are among the most important. Aquaponics, as a closed loop system consisting of hydroponics and aquaculture elements, could contribute to addressing these problems. However, there is a lack of quantitative research to support the development of economically feasible aquaponics systems. Although many studies have addressed some scientific aspects, there has been limited focus on commercial implementation. In this review paper, opportunities that have the potential to fill the gap between research and implementation of commercial aquaponic systems have been identified. The analysis shows that aquaponics is capable of being an important driver for the development of integrated food production systems. Arid regions suffering from water stress will particularly benefit from this technology being operated in a commercial environment. OPEN ACCESSSustainability 2015, 7 4200

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“…Up to 50% of the N in soybean leaves is stored in ribulose 1,5-bisphosphate (Rubisco), which is the key photosynthetic enzyme for trapping carbon dioxide (Wittenbach et al 1980). Rubisco is an especially effective means of storing N because this protein can be synthesised in abundance, it is innocuous in leaves, and it is stable until degraded by specific proteases (Hatayama et al 2000). Levels of N concentrations in soybean leaves for example, can substantially exceed the concentration at which photosynthetic rates are maximised (Lugg and Sinclair 1981).…”

Section: Nitrogen Feedback Control Of Nitrogen Fixationmentioning

confidence: 99%

The future of grain legumes in cropping systems

Sinclair

Vadez

2012

Crop Pasture Sci.

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Abstract. Grain legume production is increasing worldwide due to their use directly as human food, feed for animals, and industrial demands. Further, grain legumes have the ability to enhance the levels of nitrogen and phosphorus in cropping systems. Considering the increasing needs for human consumption of plant products and the economic constraints of applying fertiliser on cereal crops, we envision a greater role for grain legumes in cropping systems, especially in regions where accessibility and affordability of fertiliser is an issue. However, for several reasons the role of grain legumes in cropping systems has often received less emphasis than cereals. In this review, we discuss four major issues in increasing grain legume productivity and their role in overall crop production: (i) increased symbiotic nitrogen fixation capacity, (ii) increased phosphorus recovery from the soil, (iii) overcoming grain legume yield limitations, and (iv) cropping systems to take advantage of the multi-dimensional benefits of grain legumes.

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Ribulose-1,5-bisphosphate carboxylase/oxygenase from an ammonia-oxidizing bacterium, Nitrosomonas sp. K1: Purification and properties

Cited by 10 publications

References 16 publications

Response to mercury (II) ions in Methylococcus capsulatus (Bath)

Response to mercury (II) ions in Methylococcus capsulatus (Bath)

Challenges of Sustainable and Commercial Aquaponics

The future of grain legumes in cropping systems

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