Ribotype diversity of <i>Actinomyces</i> With similar intraoral tropism but different types of <i>N</i>‐acetyl‐β‐<scp>d</scp>‐galactosamine binding specificity

Hallberg, K.; Holm, Charlotta; Hammarström, K.-J.; Kalfas, Sotirios; Strömberg, Nicklas

doi:10.1111/j.1399-302x.1998.tb00731.x

Cited by 13 publications

(22 citation statements)

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“…It is noteworthy that saliva which favors the binding of S. mutans appears to occur at low frequencies (Table 1), and only few individuals are colonized with high numbers (Carlsson, 1988). Also, APRP binding is highly prevalent among early Actinomyces (Str6mberg et al, 1996;Hallberg et al, 1997) and streptococci (Hsu et al, 1994), both of which colonize most individuals in high numbers (Nyvad and Kilian, 1987). The agglutinin biological activity coincided with the S. mutans binding capacity of the three saliva samples.…”

Section: Discussionmentioning

confidence: 89%

“…The acidic, basic, and glycosylated proline-rich proteins (PRPs) are major and highly polymorphic salivary constituents which mediate bacterial adherence to oral surfaces (Gibbons and Hay, 1988;Gibbons, 1989;Lamkin and Oppenheim, 1993). Members of the genera Streptococcus and Actinomyces, both of which constitute a major portion of early dental plaque (Nyvad and Kilian, 1987), therefore bind avidly to acidic PRPs (APRPs) (Gibbons, 1989;Hsu et al, 1994;Strormberg et al, 1996;Hallberg et al, 1997). The APRP receptor determinants for the A. naeslundii strain LY7 and the S. gordonii strain Blackburn have been delineated to the ProGlndipeptide-a repetitive structure in the carboxy-terminal domain of APRPs and the statherin protein (Gibbons et al, 1991).…”

Section: Introductionmentioning

confidence: 98%

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Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces

et al. 1998

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Bacterial binding to salivary proteins may in part account for individual differences in the colonization of tooth surfaces. High-molecular-weight glycoproteins, agglutinins, mediate S. mutans adherence, whereas acidic proline-rich proteins mediate adherence of other early-colonizing streptococci and Actinomyces. The aim of the present study was to examine the composition of adherence-related salivary proteins and dental plaque micro-organisms in three individuals with a low, moderate, and high capacity to mediate S. mutans adherence. The S. mutans (strain Ingbritt) binding activity resided with a 300-kDa agglutinin which was six-fold more prevalent in the high S. mutans binding saliva compared with the low one. Binding to all three salivas was completely blocked by a monoclonal anti-agglutinin antibody. The moderate S. mutans binding saliva was found to contain adherence-inhibiting components. Furthermore, the low and moderate S. mutans binding salivas mediated binding of A. naeslundii strain LY7 to a greater extent than the saliva with high S. mutans binding. The A. naeslundii binding activity resided with the acidic proline-rich proteins (APRPs) and paralleled the relative content of 106- and 150-residue APRPs. Low A. naeslundii binding coincided with an almost two-fold higher ratio of 106/150 APRPs compared with the high A. naeslundii binding saliva. During conventional gel filtration, a degradation of the acidic, basic, and glycosylated proline-rich proteins was evident in the saliva with high S. mutans and low A. naeslundii binding. This saliva donor had a comparably high rate of dental plaque formation, high counts of S. mutans, and low counts of other streptococci and Actinomyces.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 98%

Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces

et al. 1998

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“…Among various Actinomyces spp., strains belonging to A. naeslundii remain the most heterogeneous (38). Previous workers have used different methodology to study the genetic diversity of this species, including genomic DNA fingerprinting (6, 30), ribotyping (6, 19), PCR‐RFLP with different endonuclease (26, 34), and arbitrarily primed‐polymerase chain reaction (AP‐PCR) (30).…”

Section: Discussionmentioning

confidence: 99%

Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries‐active preschool children

Tang

Samaranayake

Yip

2004

Oral Microbiology and Immunology

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A total of 991 isolates of Actinomyces naeslundii were obtained from sound approximal tooth sites in either caries-active (n = 35) or caries-free (n = 20) preschool children. From this group of isolates, 101 strains were chosen to study the genotypic diversity of A. naeslundii genospecies 1 (n = 30), catalase-positive (n = 30), and catalase-negative genospecies 2 (n = 41). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), with a pair of primers targeting the 16S ribosome RNA gene (16S rDNA), and MnlI digestion together with randomly amplified polymorphic DNA (RAPD) with eight arbitrary, single 10-mer primers were performed to generate genetic profiles of selected Actinomyces isolates. The hierarchic relationships of genetic profiles were finally analyzed using computerized dendrograms. There was no significant difference in the prevalence rates and proportions of either genospecies 1 or 2 between the caries-free and caries-active groups, although a higher prevalence of genospecies 2 was noted in the total population. Dendrogram analyses of the 16S rDNA PCR-RFLP profiles revealed that all strains belonging to A. naeslundii genospecies 1 could be subgrouped into three genotypes (T7, T18, and T19), with a single predominant genotype, T18 (27/30). Catalase-positive strains for genospecies 2 fell into three subtypes (T4, T7, and T17), whereas the catalase-negative counterparts were distributed amongst 16 subtypes. No specific genotype was significantly associated with caries activity. We conclude that heterogeneous subgroups of A. naeslundii genospecies 1 and 2, particularly the latter, are the constituent flora of dental plaque in children and may contribute to the pathogenesis of childhood caries.

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“…Previous studies have also reported on the intra- and inter-species diversities of A. naeslundii and A. oris , as A. naeslundii genospecies 1 and 2 using DNA fingerprinting [11], ribotyping [5], [12], amplified 16S ribosomal DNA restriction analysis (ARDRA) [4], [13], DNA probes [14], sequence analysis of fimA genes [9] and REP-PCR amplicons analysis [10]. All these studies have demonstrated extensive heterogeneity within each taxon.…”

Section: Introductionmentioning

confidence: 99%

Application of MLST and Pilus Gene Sequence Comparisons to Investigate the Population Structures of Actinomyces naeslundii and Actinomyces oris

Henssge

Gilbert

et al. 2011

PLoS ONE

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Actinomyces naeslundii and Actinomyces oris are members of the oral biofilm. Their identification using 16S rRNA sequencing is problematic and better achieved by comparison of metG partial sequences. A. oris is more abundant and more frequently isolated than A. naeslundii. We used a multi-locus sequence typing approach to investigate the genotypic diversity of these species and assigned A. naeslundii (n = 37) and A. oris (n = 68) isolates to 32 and 68 sequence types (ST), respectively. Neighbor-joining and ClonalFrame dendrograms derived from the concatenated partial sequences of 7 house-keeping genes identified at least 4 significant subclusters within A. oris and 3 within A. naeslundii. The strain collection we had investigated was an under-representation of the total population since at least 3 STs composed of single strains may represent discrete clusters of strains not well represented in the collection. The integrity of these sub-clusters was supported by the sequence analysis of fimP and fimA, genes coding for the type 1 and 2 fimbriae, respectively. An A. naeslundii subcluster was identified with both fimA and fimP genes and these strains were able to bind to MUC7 and statherin while all other A. naeslundii strains possessed only fimA and did not bind to statherin. An A. oris subcluster harboured a fimA gene similar to that of Actinomyces odontolyticus but no detectable fimP failed to bind significantly to either MUC7 or statherin. These data are evidence of extensive genotypic and phenotypic diversity within the species A. oris and A. naeslundii but the status of the subclusters identified here will require genome comparisons before their phylogenic position can be unequivocally established.

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Ribotype diversity of Actinomyces With similar intraoral tropism but different types of N‐acetyl‐β‐d‐galactosamine binding specificity

Cited by 13 publications

References 22 publications

Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces

Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces

Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries‐active preschool children

Application of MLST and Pilus Gene Sequence Comparisons to Investigate the Population Structures of Actinomyces naeslundii and Actinomyces oris

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