Ribosome-tetracycline interactions

Tritton, Thomas R.

doi:10.1021/bi00637a029

Cited by 81 publications

(42 citation statements)

References 21 publications

(21 reference statements)

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“…Tetracycline appears to have a single tight-binding site on the ribosome as well as a multitude of weaker ones. Although the point is disputed in the literature [5,14], most of the evidence suggests that inhibition is correlated with tight-site binding of TC [14,15]. The results presented in fig.3 and table 4 are consistent with the proposition that the observed photoinduced inactivation of ribosomes is a direct consequence of the incorporation of 1 TC/ribosome.…”

Section: Discussionsupporting

confidence: 82%

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Photoincorporation of tetracycline into Escherichia coli ribosomes

et al. 1980

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Section: Discussionsupporting

confidence: 82%

“…70 S ribosomes were prepared from both E. coli MRE600 [5] and E. coli Q13 [6] as described. Large scale preparations of 30 S and 50 S subunits were obtained by zonal centrifugation in a Beckman Ti 15 rotor essentially as in [7].…”

Section: Methodsmentioning

confidence: 99%

Photoincorporation of tetracycline into Escherichia coli ribosomes

et al. 1980

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“…In this situation, only about 2.5% of total drug associated with cells is free. Second, much more drug is associated with each cell than is needed to inhibit the 15,000 ribosomes (one drug molecule per ribosome [34]). At the MIC (6 ,uM), net minocycline accumulation in the absence of energy in cells grown in L broth is about 3.6 x 10' molecules per cell; with energy, only half of this amount is excreted (Fig.…”

Section: Resultsmentioning

confidence: 99%

Susceptible Escherichia coli cells can actively excrete tetracyclines

McMurry

Aronson

Levy

1983

Antimicrob Agents Chemother

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Escherichia coli shows severalfold less susceptibility to tetracyclines when grown in enriched medium than in minimal medium. Transport studies with cells harvested from these media showed different handling of the drugs. Whereas an energy-dependent uptake oftetracycline and minocycline was observed in susceptible K-12 and wild-type E. coli strains grown in a medium, an active efflux of minocycline and, to a lesser extent, tetracycine was seen in cells grown in L broth and other enriched media. This efflux was replaced by an active uptake system after treatment of cells grown in L broth with EDTA. When assayed at lower temperature (270C), even cells grown in minimal medium showed an efflux of minocycline. Everted membrane vesicles pgepared from susceptible cells grown in minimal medium or L broth showed. an energy-dependent accumulation of nunocycline and tetracycline when supplied with certain divalent cations. These results suggest that an active efflux of tetracyclines occurs in susceptible E. coli but is not detected in cells grown in minimal medium because greater permeability of the outer membrane allows a more rapid active uptake. This efflux system is distinct from that specified by tetracycline resistance determinants. Since the active efflux of minocycline in cells grown in L broth disappeared at external antibiotic concentrations of >100 ,uM, it may be saturable and so mediated by a membrane carrier.More is known about how a bacterium takes up needed moeules or ions from its environment than how it excretes-them. Among the latter processes are energy-dependent efflux mechanisms for sodium (30), calcium (6, 28) and protons (15,27

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“…The exact mechanism of their drug action, so far, has not been proposed. However, they d o form coordination complexes with metal ions in vitro (10)(11)(12). The results of ultrasonic, volumetric, and viscornetric studies of tetracycline hydrochloride and chlorotetracycline in water and water-dioxan system are presented in this work.…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic and thermodynamic studies of tetracyclines in solutions

Pandey¹,

Mishra²,

Shukla³

et al. 1987

Can. J. Chem.

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. Can. J. Chem. 65, 303 (1987).The ultrasonic velocity, U , density, and viscosity of aqueous and non-aqueous (water + dioxan) solutions of tetracycline hydrochloride and chlorotetracycline have been measured at two different temperatures. Partial molal volumes, $, , partial molal compressibility, $kr Vand's interaction coefficients, Q and C , and thermodynamic activation parameters AG*, AS*, and AH* of these solutions have been computed from the ultrasonic velocity, density, and viscosity data. The results are interpreted in terms of solute-solvent interaction.

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Ribosome-tetracycline interactions

Cited by 81 publications

References 21 publications

Photoincorporation of tetracycline into Escherichia coli ribosomes

Photoincorporation of tetracycline into Escherichia coli ribosomes

Susceptible Escherichia coli cells can actively excrete tetracyclines

Ultrasonic and thermodynamic studies of tetracyclines in solutions

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