Ribosome Display Technology: Applications in Disease Diagnosis and Control

Abstract

Antibody ribosome display remains one of the most successful in vitro selection technologies for antibodies fifteen years after it was developed. The unique possibility of direct generation of whole proteins, particularly single-chain antibody fragments (scFvs), has facilitated the establishment of this technology as one of the foremost antibody production methods. Ribosome display has become a vital tool for efficient and low-cost production of antibodies for diagnostics due to its advantageous ability to screen large libraries and generate binders of high affinity. The remarkable flexibility of this method enables its applicability to various platforms. This review focuses on the applications of ribosome display technology in biomedical and agricultural fields in the generation of recombinant scFvs for disease diagnostics and control.

The impact of AlphaFold on experimental structure solution

“…An obvious translational outlet from de novo protein design would be the creation of “biologics”, such as antibodies 17 or DARPins 18 . To date, creation of such molecules has favoured time- and resource-intensive experimental techniques such as ribosome-scanning 19 . If design of biologic therapeutics could be carried out computationally, this would represent a huge advance for potential therapeutics but also research and diagnostic tools.…”

Hybridoma technology: is it still useful?

“…The diversity of the phage library depends on the bacterial transformation efficiency and is limited to the 10 10 -10 11 variant antibody maximum repertoire of the phage display library. This restriction can be overcome by mRNA and ribosome display strategies, which are in vitro cell-free methods having a bigger library size and a higher displayed antibody diversity (10 14 variants) ( Hudson and Souriau, 2003 ; Kunamneni et al., 2020 ). It should be also considered that phage display-selected mAbs are generated in E. coli and therefore are not glycosylated; the use of eukaryotic display platforms, like yeast ( Doerner et al., 2014 ) and mammalian expression systems ( Zhu and Hatton, 2017 ), is a possibility to circumvent that.…”

Phage Display Technology as a Powerful Platform for Antibody Drug Discovery

“…Therefore, the development of phage display technology provides optimal sequences to target peptides or proteins, unlike conventional alanine scanning and other methods, and aids understanding of their molecular evolution [ 2 , 3 , 4 , 5 , 6 , 7 ]. Although various kinds of molecular display technologies such as ribosome display [ 8 , 9 ], and yeast display technologies [ 10 , 11 ] have been proposed, phage display technology is frequently employed because of a high diversity of molecules that can be displayed and ease of handling [ 12 , 13 ].…”