Ribosome biogenesis and p53: Who is regulating whom?

Krastev, Dragomir B.; Buchholz, Frank

doi:10.4161/cc.10.20.17687

Cited by 5 publications

(1 citation statement)

References 7 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high-energy cost of ribosome biogenesis dominates the G1 phase of eukaryotic cells (Warner, 1999;Krastev and Buchholz, 2011) and as a consequence ribosome production is tightly linked to cell cycle progression. The cell cycle checkpoints that monitor this synthetic pathway operate by sensing changes in ribosome biogenesis prior to any significant loss of protein biosynthetic capacity (Volarevic et al, 2000;Pestov et al, 2001;Bernstein et al, 2007;Hutson et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

A nucleolar AAA‐NTPase is required for parasite division

Suvorova

Radke

Ting

et al. 2013

Molecular Microbiology

View full text Add to dashboard Cite

Summary Apicomplexa division involves several distinct phases shared with other eukaryote cell cycles including a gap period (G1) prior to chromosome synthesis, although how progression through the parasite cell cycle is controlled is not understood. Here we describe a cell cycle mutant that reversibly arrests in the G1 phase. The defect in this mutant was mapped by genetic complementation to a gene encoding a novel AAAATPase/CDC48 family member called TgNoAP1. TgNoAP1 is tightly regulated and expressed in the nucleolus during the G1/S phases. A tyrosine to a cysteine change upstream of the second AAA+ domain in the temperature sensitive TgNoAP1 allele leads to conditional protein instability, which is responsible for rapid cell cycle arrest and a primary defect in 28S rRNA processing as confirmed by knock-in of the mutation back into the parent genome. The interaction of TgNoAP1 with factors of the snoRNP and R2TP complexes indicates this protein has a role in pre-rRNA processing. This is a novel role for a cdc48-related chaperone protein and indicates that TgNoAP1 may be part of a dynamic mechanism that senses the health of the parasite protein machinery at the initial steps of ribosome biogenesis and conveys that information to the parasite cell cycle checkpoint controls.

show abstract