Ribosomal small-subunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China

Schnittger, Leonhard; Yin, Hong; Luo, Jianxun; Ludwig, Wolfgang; Shayan, Parviz; Rahbari, S; Voss-Holtmann, A.; Ahmed, Jabbar S.

doi:10.1007/s004360050680

Cited by 75 publications

(70 citation statements)

References 26 publications

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“…This gene has been used to identify and classify several previously unknown Theileria and Babesia parasites (4,12,28,29,31). An RLB assay based on species-specific sequences in parasite 18S rRNA genes has been developed for the simultaneous detection and identification of tick-borne parasites infecting cattle and small ruminants (3,11,32).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

et al. 2008

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Babesiosis in a sable antelope (Hippotragus niger Harris, 1838) was first reported in 1930; the parasite was named Babesia irvinesmithi. Recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. Microscopic examination of thin blood smears revealed the presence of small piroplasms. DNA was extracted from blood samples; the V4 variable region of the 18S rRNA gene was amplified and analyzed using the reverse line blot (RLB) assay. Amplicons did not hybridize with any of the Babesia or Theileria species-specific probes present on the blot and hybridized only with a Babesia or Theileria genus-specific probe, suggesting the presence of a novel species. The full-length 18S rRNA gene sequence was obtained and aligned with published sequences of related genera, and phylogenetic trees were constructed. Sequence similarity analyses indicated that a Babesia species, designated Babesia sp. (sable), was present. The sequence showed its highest similarity to B. orientalis and to an unnamed Babesia species previously detected in bovine samples. The latter was later established to be Babesia occultans. A Babesia sp. (sable)-specific RLB oligonucleotide probe was designed and used to screen 200 South African sable samples, but so far, no other sample has been found to be positive for the presence of Babesia sp. (sable) DNA. In summary, we identified a novel piroplasm parasite from a sable antelope that died from an unknown illness. While the parasite was observed in blood smears, there is no direct evidence that it was the cause of death.

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Section: Discussionmentioning

confidence: 99%

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

et al. 2008

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“…Of the two species infective to small ruminants, one isolate from Lintan Gansu has been analyzed before (Schnittger et al 2000), and together with three other isolates, the parasite was designated Theileria sp.…”

Section: Discussionmentioning

confidence: 99%

“…This led to a more precise classification of some unknown Theileria spp., especially those benign Theileria spp. infective to cattle (Ellis et al 1992;Allsopp et al 1993Allsopp et al , 1994Chae et al 1998;Kakuda et al 1998;Katzer et al 1998;Chansiri et al 1999;Kawazu et al 1999;Schnittger 2000, Gubbles et al 20002002).…”

Section: Introductionmentioning

confidence: 97%

“…However, recent biological and molecular studies (Schnittger et al 2000;Yin et al 2002aYin et al , 2002bYin et al , 2003 suggested that this parasite could not transform cells in vitro and that schizonts were very difficult to detect in biopsy smears of lymph node. In addition, it was different from T. annulata and T. lestoquardi, and in an inferred phylogenetic tree, the 18S ssrRNA gene sequence of this parasite was most closely related to T. buffeli and clearly divergent from T. lestoquardi and T. annulata.…”

Section: Introductionmentioning

confidence: 98%

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Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghaiensis

Yin

Luo

Schnittger

et al. 2004

Parasitology Research

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The phylogenetic relationships between six isolates of Theileria spp. infective to small ruminants, and two isolates of Theileria spp. infective to yak, all transmitted by Haemaphysalis qinghaiensis, together with the Theileria orientalis/sergenti/buffeli group and T. sinensis, were analyzed using the 18S ssrRNA gene sequence. The target DNA segment was amplified by polymerase chain reaction (PCR). The PCR product was used either for direct sequencing or was ligated to the PCR II vector for sequencing. The length of the 18S ssrRNA gene of all Theileria spp. involved in this study was around 1,740 bp. Two phylogenetic trees were inferred based on the 18S ssrRNA gene sequence of the Chinese isolates only, and Chinese isolates and other species of Theileria available in GenBank. In the first tree, the Theileria sp. infective to yaks was found to be T. sinensis. The Theileria sp. infective to small ruminants was found to be composed of two separate species of Theileria. Theileria sp. from Qinghai, Madang, Ningxian and Lintan, which was identical to the unidentified Theileria sp. described previously, is designated Theileria sp (China 1). The Theileria sp. from Longde, Zhangjiachuan and Lintan, which has not been described previously, is designated Theileria sp. (China 2) in order to avoid confusion. In the second tree, Theileria sp. (China 1) was closely related to benign Theileria, such as T. buffeli and T. sergenti, while Theileria sp. (China 2) was separated from other Theileria spp. The results indicate that H. qinghaiensis transmit at least three species of Theileria, two which are infective to sheep and goats, but not yak and one which is infective to yaks and cattle, but not to sheep and goats.

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“…The 18S rRNA genes have been successfully applied to identify and classify several previously unknown Theileria spp., especially the benign Theileria spp. which infects ruminants [14,18]. Thus, the nested PCR based on the 18S rRNA gene is an alternative molecular diagnostic tool that enables detection of mixed infections of ovine Theileria spp.…”

mentioning

confidence: 99%

Molecular Detection of <i>Theileria</i> Species in Sheep from Northern China

Cao

Shou-fa

Jia

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Ovine theileriosis is a tick-borne disease that restricts the development of small ruminant husbandry in northern China. In this study, we report on a molecular epidemiological survey of ovine Theileria spp. in 198 blood samples taken from sheep in northern China. The DNA samples were screened by a nested polymerase chain reaction (PCR) targeting the 18S rRNA gene of ovine Theileria spp. The prevalence of ovine Theileria spp. in Yanji, Nongan, Longjing, Toudao and Jinchang was 80%, 40%, 37%, 24% and 32%, respectively. The sequencing analyses approved the present of the T. orientalis and/or T. luwenshuni in these regions. Taken together, we have demonstrated a high incidence of Theileria spp. in northern China that calls for the need to design effective control programs for ovine theileriosis.

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Ribosomal small-subunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China

Cited by 75 publications

References 26 publications

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)

Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghaiensis

Molecular Detection of <i>Theileria</i> Species in Sheep from Northern China

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