Ribosomal RNA synthesis in mitochondria of Neurospora crassa

Kuriyama, Yoshiaki; Luck, David

doi:10.1016/0022-2836(73)90091-0

Cited by 64 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present such information is limited almost entirely to the ribosomal and transfer RNA genes. In N. crassa the ribosomal RNAs are transcribed as a single 328 precursor (123) and hybridization of the mature RNAs to restriction fragments indicates that they have a proximal location on the genome (93). This also appears to be true of most animal mitochondrial genomes (124,125).…”

Section: Constancy Of the Mitochondrial Genomementioning

confidence: 99%

Mitochondrial Genes and Translation Products

Tzagoloff¹,

Macino²

1979

Annu. Rev. Biochem.

245

View full text Add to dashboard Cite

Section: Constancy Of the Mitochondrial Genomementioning

confidence: 99%

Mitochondrial Genes and Translation Products

Tzagoloff¹,

Macino²

1979

Annu. Rev. Biochem.

245

View full text Add to dashboard Cite

“…Previous reports on the hybridization of mitochondrial ribosomal RNA with mtDNA indicated that the DNA contains one gene for the large and one gene for the small rRNA [2,5,6]. Hybridization of the rRNAs to restriction fragments of the mtDNA, described in this article, shows that the genes for the large and small rRNA are located close to each other on the mitochondrial genome.…”

Section: Introductionmentioning

confidence: 59%

The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Terpstra

Holtrop

Kroon

1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

View full text Add to dashboard Cite

“…In addition, the glyoxal denaturation experiments (Fig. 5) that 32S RNA is not a continuous polynucleotide chain.,However, reexamination of the data of Kuriyama and Luck (5),-even in the most critical light, makes it seem clear that: (i) RNA molecules with electrophoretic mobility equivalent to 32S are present in gels under nondenaturing conditions; (ii) these molecules contain roughly equal concentrations of 19S and 25S RNA sequences; and (iii) at least some are precursors as judged by pulse and pulse-chase experiments. In addition, mitochondrial ribosomal precursor particles that appear to consist of 32S RNA associated with newly synthesized small and large subunit proteins have been identified on sucrose gradients (29).…”

mentioning

confidence: 99%

“…1. in which the 19S and 25S RNA genes are sef)arate(I lby [5][6] kb. suggests that :32S RNA is not a continuous transcript ol 19S and 25S RNA as proposed by Kuriyama and Ltuck (3).…”

mentioning

confidence: 99%

“…Strip filter hybridization was carried out by the method of Southern (17). Restriction enzyme fragments (4)(5)(6) Mg per slot) were separated by electrophoresis on 0.8% agarose slab gels (21 cm long, 0.3 cm thick) as described (14). Unless otherwise indicated, mtRNAs for strip filter hybridization were extracted by using procedure b and purified by gel electrophoresis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa.

Mannella¹,

Collins²,

Green³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Recent studies have shown that the gene encoding the large (25 S) mitochondrial rRNA of Neurospora crassa contains an intervening sequence of 2-2.5 kilobases that is not present in the mature 25S mitochondrial rRNA. Earlier studies had provided evidence that mitochondrial rRNAs in Neurospora are synthesized via a 32S precursor RNA that contains sequences for both the mature 19S and 25S RNA species. The present work shows that the intervening sequence is not present in 32S RNA. However, we have identified two temperature-sensitive nuclear mutants that fail to excise the intervening sequence at the nonpermissive temperature (370C). When grown at 370C, the mutants show decreased ratios of 25S to 19S RNA and accunitulate a novel 35S RNA that appears to consist of 25S RNA plus most or all of the intervening sequence. The mutants are allelic but can be distinguished in temperature shift-up experiments, initochondrial rRNA processing turning off more rapidly in one than in the other. These mutants should provide powerful new tools for studying RNA processing in eukaryotie cells.Recent restriction enzyme mapping of Neurospora crassa untDNA suggests that the gene coding for the large (25 S) rRNA contains an intervening sequence of 2-2.5 kilobases (kb) (ref.I and 1i J. leckman and U. RajBhandary, personal commiunication). The current map (Fig. 1 (personal communication) and supported further by the data j)resented in this paper. The experimental evidence on which the mnap is based is summarized in the legend of Fig. 1

show abstract

Ribosomal RNA synthesis in mitochondria of Neurospora crassa

Cited by 64 publications

References 22 publications

Mitochondrial Genes and Translation Products

Mitochondrial Genes and Translation Products

The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa.

Contact Info

Product

Resources

About