Ribosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization

Małuszyńska, Jolanta; Schweizer, Dieter

doi:10.1038/hdy.1989.8

Cited by 84 publications

(43 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, rDNA is subject to very rapid copy number evolution between species [53][54][55] and within species [56][57][58]. It can extrachromosomally circularize [59,60], attract transposable elements [58,61] as well as invade other selfish elements, such as B chromosomes [62][63][64] and germline-limited DNA [65]. Finally, rRNA gene clusters are recombinational hotspots in cancer [66], and abnormalities in the nucleolar morphology of cancer cells attracted the attention of tumour pathologists as early as the nineteenth century [67,68].…”

Section: Discussion (A) Number Of Nucleoli and 45s Rdna Expression Pamentioning

confidence: 99%

Nucleolar dominance and maternal control of 45S rDNA expression

et al. 2015

View full text Add to dashboard Cite

Using a system of interspecies hybrids, trihybrids, and recombinants with varying proportions of genomes from three distinct Xenopus species, we provide evidence for de novo epigenetic silencing of paternal 45S ribosomal ribonucleic acid (rRNA) genes and their species-dependent expression dominance that escapes transcriptional inactivation after homologous recombination. The same pattern of imprinting is maintained in the offspring from mothers being genetic males (ZZ) sex-reversed to females, indicating that maternal control of ribosomal deoxyribonucleic acid (rDNA) expression is not sex-chromosome linked. Nucleolar dominance (nucleolus underdevelopment) in Xenopus hybrids appears to be associated with a major non-Mendelian reduction in the number of 45S rDNA gene copies rather than a specific pattern of their expression. The loss of rRNA gene copies in F 1 hybrids was non-random with respect to the parental species, with the transcriptionally dominant variant preferentially removed from hybrid zygotes. This dramatic disruption in the structure and function of 45S rDNA impacts transcriptome patterns of small nucleolar RNAs and messenger RNAs, with genes from the ribosome and oxidative stress pathways being among the most affected. Unorthodoxies of rDNA inheritance and expression may be interpreted as hallmarks of genetic conflicts between parental genomes, as well as defensive epigenetic mechanisms employed to restore genome integrity.

show abstract

Section: Discussion (A) Number Of Nucleoli and 45s Rdna Expression Pamentioning

confidence: 99%

Nucleolar dominance and maternal control of 45S rDNA expression

et al. 2015

View full text Add to dashboard Cite

show abstract

“…These elements are potentially B-chromosomes. The fact that in some well-studied cases it has been possible to demonstrate the existence of transcription involving nucleolar organizer regions (Cabrero et a!., 1987;Maluszynska & Schweizer, 1989) indicates an ancestral relationship with the A chromosomes. The results obtained in E. plorans also suggest this ancestral resemblance.…”

Section: Taqimentioning

confidence: 99%

Autosomal, sex and B chromosomes in Eyprepocnemis plorans (Orthoptera) viewed with restriction endonuclease in situ digestion

1992

View full text Add to dashboard Cite

Similarities and differences between different subsets of heterochromatin localized on the autosomes, sex chromosomes and a B-chromosome in the genome of the grasshopper Eyprepocnemis plorans were analysed by in-situ digestion with 23 different restriction endonucleases, three unspecific nucleases, C-banding and specific fluorescent DNA-ligands. Results show an equilocal distribution of the different heterochromatic subsets in this species and provide a possible evolutionary origin for the B chromosome.

show abstract

“…The plants used were descendants of original plant and seed material collected from three areas near Port Augusta in South Australia during the spring of 1989and 1990(John et a!., 1991. Brachycome dichromosomatica is an outbreeding species and abundant seed can readily be obtained by rubbing flowering heads together.…”

Section: P/ant Materialsmentioning

confidence: 99%

“…The method of Maluszynska & Schweizer (1989) was modified as follows. Slides were incubated for 10 mm in 0.01 M borate buffer (pH 9.2), 200 1uL 1: 1 w/v AgNO3 in water was Millipore filtered and added to each slide, which was covered with a nylon mesh and incubated at 65°C for up to 2 h. Slides were rinsed, 200 uL 3:2 AgNO3: 2 per cent gelatine 1 per cent formic acid (pH 3.0) added and incubated for 10 mm.…”

Section: Silver Stainingmentioning

confidence: 99%

See 1 more Smart Citation

Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica

et al. 1995

View full text Add to dashboard Cite

Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachyconie dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed.Southern hybridization of DNA from OB and + B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.

show abstract

Ribosomal RNA genes in B chromosomes of Crepis capillaris detected by non-radioactive in situ hybridization

Cited by 84 publications

References 28 publications

Nucleolar dominance and maternal control of 45S rDNA expression

Nucleolar dominance and maternal control of 45S rDNA expression

Autosomal, sex and B chromosomes in Eyprepocnemis plorans (Orthoptera) viewed with restriction endonuclease in situ digestion

Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica

Contact Info

Product

Resources

About