Ribosomal RNA gene patterns of<i>Helicobacter pylori</i>from surgical patients with healed and recurrent peptic ulcers

Owen, Robert J.; Bickley, J.; Lastovica, Albert J.; Dunn, J. P.; Borman, P.; Hunton, C.

doi:10.1017/s0950268800049487

Cited by 21 publications

(10 citation statements)

References 27 publications

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“…We studied the polymorphism of total DNA using the ribotyping method. Ribotyping seems to be a highly sensitive method for H. pylori strain identification [28, 29] even if only two copies of the rDNA operon are present [30]. We identified 38 ribotypes among the 39 strains.…”

Section: Discussionmentioning

confidence: 93%

Panmictic structure ofHelicobacter pyloridemonstrated by the comparative study of six genetic markers

Salaün

Audibert

Lay

et al. 1998

FEMS Microbiology Letters

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We compared the classifications of strains obtained by analysis of several genetic markers to demonstrate the panmictic structure of Helicobacter pylori, previously suggested by the study of multilocus enzyme electrophoresis. A series of 39 strains, including 37 clinical isolates from patients with gastritis or ulcers from two regions of France, reference strain CIP 101260 and the Sydney strain (strain SSI), were used. They were studied by restriction fragment length polymorphism analysis of ribosomal DNA (ribotyping) using HindIII and HaeIII, by polymorphism analysis of the ureA-ureB and flaA genes by PCR-RFLP using HaeIII and MboI, by vacA genotyping and by the presence or absence of the cagA gene and of the insertion sequence IS605 detected by PCR. There was a high level of genetic polymorphism over the studied strains, with 38 ribotypes, 38 restriction profiles for the ureA-ureB gene, 19 restriction profiles for the flaA gene and five combinations of the signal and mid-region sequences of the vacA gene. Factorial analysis of correspondence and hierarchical clustering performed using each marker revealed that the different classifications of the strains were not correlated. This suggests there is much genetic recombination between strains and supports the hypothesis of a panmictic structure for the H. pylori species.

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Section: Discussionmentioning

confidence: 93%

Panmictic structure ofHelicobacter pyloridemonstrated by the comparative study of six genetic markers

Salaün

Audibert

Lay

et al. 1998

FEMS Microbiology Letters

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“…Previous studies of H. pylori from different countries using chromosomal DNA fingerprinting (total restriction digest patterns and ribopatterns) showed the species was genomically diverse, and that almost every person was colonized by a unique strain (15,16,18,19). As individual strain ribopatterns are stable and highly reproducible despite frequent subculturing, they provide reliable molecular markers (18).…”

Section: Variation Within Individualsmentioning

confidence: 99%

Comparisons between degree of histological gastritis and DNA fingerprints, cytotoxicity and adhesivity of Helicobacter pylori from different gastric sites

Owen¹,

Desai²,

Figura

et al. 1993

Eur J Epidemiol

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Thirty-six isolates of H. pylori from up to three gastric biopsy sites (antrum, corpus and fundus) from 13 patients in Italy with different degrees of histological gastritis were investigated. All strains were tested for motility, cytotoxicity and degree of adhesion, and were typed by analysis of ribosomal RNA gene patterns (ribopatterns). Seventeen different DNA types (ribotypes) were identified, with each patient possessing H. pylori of one or more unique types. Only two patients had identical H. pylori at three sites. Most patients had H. pylori with different ribotypes or subtypes, but nine strains were not typable. Five patients had the same strain colonizing two of the three sites and atypical strains were mostly from the antrum. A complex pattern of H. pylori colonization in the stomach of some individuals was evident and suggested multiple sources of infection. No consistent associations were detected between degree of gastritis and adherence, cytotoxicity and motility but a 2.56Kb rRNA gene fragment that had a higher frequency in strains associated with severe gastritis than mild gastritis, may provide a useful molecular marker for future pathogenicity studies.

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“…The ribosomal ribonucleic acid gene restriction patterns are investigated by Southern blotting followed by hybridization with a labelled ribosomal RNA probe [18]. Non‐radioactive detection systems with biotinylated or digoxigenin‐marked probes have been used [19–22]. The major disadvantage of the method is that it is time‐consuming (Table 1).…”

Section: Ribotypingmentioning

confidence: 99%

Molecular methods for typing ofHelicobacter pyloriand their applications

Colding

Hartzen²,

Roshanisefat

et al. 1999

FEMS Immunology &Amp; Medical Microbiology

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Microbial typing is a useful tool in clinical epidemiology for defining the source and route of infection, for studying the persistence and reinfection rates, clonal selection in the host and bacterial evolution. Phenotypic methods such as biotyping, serotyping and hemagglutinin typing have little discriminatory power compared to genotypic methods concerning the typing of Helicobacter pylori. Therefore great efforts have been made to establish useful molecular typing methods. In this context, the most frequently used genotypic methods are described based on our own experience and the literature: (1) restriction endonuclease analysis, (2) endonuclease analysis using pulsed-field gel electrophoresis, (3) ribotyping, (4) polymerase chain reaction (using either random primers or repetitive DNA sequence primers), and (5) polymerase chain reaction-restriction fragment length polymorphism analysis of e.g. the urease genes. Furthermore, reproducibility, discriminatory power, ease of performance and interpretation, cost and toxic procedures of each method are assessed. To date no direct comparison of all the molecular typing methods described has been performed in the same study with the same H. pylori strains. However, PCR analysis of the urease gene directly on suspensions of H. pylori or gastric biopsy material seems to be useful for routine use and applicable in specific epidemiological situations.

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Ribosomal RNA gene patterns ofHelicobacter pylorifrom surgical patients with healed and recurrent peptic ulcers

Cited by 21 publications

References 27 publications

Panmictic structure ofHelicobacter pyloridemonstrated by the comparative study of six genetic markers

Panmictic structure ofHelicobacter pyloridemonstrated by the comparative study of six genetic markers

Comparisons between degree of histological gastritis and DNA fingerprints, cytotoxicity and adhesivity of Helicobacter pylori from different gastric sites

Molecular methods for typing ofHelicobacter pyloriand their applications

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