Ribosomal Resistance to Streptomycin and Spectinomycin in
            <i>Neisseria gonorrhoeae</i>

Maness, Michael J.; Foster, Gayle C.; Sparling, P. Frederick

doi:10.1128/jb.120.3.1293-1299.1974

Cited by 45 publications

(12 citation statements)

References 22 publications

(25 reference statements)

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“…Since the str and spc loci are known to be structural genes for ribosomal proteins in E. coli and B. subtilis (3), it is a reasonable supposition that the str and spc loci in the gonococcus might also be part of a cluster of ribosomal genes. This inference is documented in an accompanying paper (8).…”

Section: Discussionmentioning

confidence: 76%

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Genetic Mapping of Linked Antibiotic Resistance Loci in Neisseria gonorrhoeae

1974

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Loci for resistance to several antibiotics in laboratory-derived strains of Neisseria gonorrhoeae were mapped by genetic transformation. Genes for high-level resistance to streptomycin (str) and spectinomycin (spc) and for low-level resistance to tetracycline (tet) and chloramphenicol (chl) were linked. Also, a locus for high-level resistance to rifampin (rinf was linked to str and tet. The apparent order was rif . .. str . . tet . . . chi. . . spc. Loci for resistance to other antibiotics (penicillin, erythromycin) were transferred independently of each other and were not linked to the cluster around str. Similar linkage relationships were found with str, tet, chl, and spc loci obtained from naturally occurring (clinical) isolates of N. gonorrhoeae.suspended to a density of approximately 5 x 107 colony-forming units per ml (after vigorous agitation to disrupt most clumps) in 0.90 ml of GCBB containing 0.002 M CaCl,. No attempt was made to selec-1284 on July 31, 2020 by guest

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Section: Discussionmentioning

confidence: 76%

“…Linkage was demonstrated for five genes, four of which determine resistance to antibiotics which act on the ribosome. In an accompanying paper, we demonstrate that two of the genes in this cluster affect the structure of the 30S ribosomal subunit (8).…”

mentioning

confidence: 78%

Genetic Mapping of Linked Antibiotic Resistance Loci in Neisseria gonorrhoeae

1974

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“…The multiple transferable resistance (Mtr) locus later characterized as efflux system PWUCDE was described by Maness and Sparling [107,108] and exports hydrophobic agents among them fatty acids, penicillin, tetracycline and macrolides [91,104,109,110] and belongs to the RND-family [105]. MtrR represses expression of PWU&'( [111].…”

Section: And(37%/7mentioning

confidence: 99%

Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture

Airell

Lindbäck²,

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et al. 2005

Int J STD AIDS

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We compared 956 samples for AMPLICOR Neisseria gonorrhoeae polymerase chain reaction (PCR) (Roche) with species verification using the 16S rRNA gene to verification using gyrA gene. Control was the culture method. The gyrA verification uses pyrosequencing of the quinolone resistance-determining region of gyrA. Of 52 samples with optical density >/=0.2 in PCR, 27 were negative in culture, two samples from pharynx were false negative in culture and four samples from pharynx were false positives in verification with 16S rRNA. Twenty-five samples showed growth of gonococci, 18 of the corresponding PCR samples were verified by both methods; three urine samples were positive only in gyrA ; and one pharynx specimen was positive only in 16S rRNA. Three samples were lost. We conclude that AMPLICOR N. gonorrhoeae PCR with verification in gyrA gene can be considered as a diagnostic tool in populations with low prevalence of gonorrhoea and that pharynx specimens should not be analysed by PCR.

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“…Thus, a transformation system developed for the alternative spectinomycin marker is important for the practical application of such genetic identification. Both genetic markers are ribosomal (9), and one-step mutants to high levels of resistance are most often easily obtained in the laboratory.…”

Section: E Corrodens As the Name Implies Is Characterized By Corromentioning

confidence: 99%