Ribosomal Protein uS5 and Friends: Protein–Protein Interactions Involved in Ribosome Assembly and Beyond

Landry-Voyer, Anne-Marie; Hassani, Zabih Mir; Avino, Mariano; Bachand, François

doi:10.3390/biom13050853

Cited by 5 publications

(3 citation statements)

References 100 publications

(208 reference statements)

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“…As we all know, ribosomal proteins play an important part in ribosome assembly. They combine with four ribosomal RNAs (rRNAs) to form the ribosomal subunits, which have an important function cellular protein translation ( Landry-Voyer et al, 2023 ). Consistent with our findings, reference genes involving ribosomal protein genes have been selected frequently for expression analysis in various insects during the last 10 years ( Zhang et al, 2022 ; Shen et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Shen,

Tang,

et al. 2024

Front. Physiol.

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Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited. In this study, ten internal reference genes (ACT, ARF1, AK, EF1α, GAPDH, α-TUB, RPL6, RPL13, RPS3 and RPS18) were tested and assessed under four selected treatments including adult ages, adult tissues, temperatures, and sex by RT-qPCR based on five methods (Ct value, geNorm, NormFinder, BestKeeper and RefFinder). Our findings showed that RPL6 and RPL13 were the most optimal internal reference gene combination for gene expression during various adult ages and under diverse temperatures; The combination of RPL6 and RPS18 was recommended to test gene transcription levels under different adult tissues. AK and RPL6 were the best reference genes in male and female adults. RPL6 and RPL13 were the most appropriate reference gene pair to estimate gene expression levels under four different tested backgrounds. The relative transcript levels of a uridine diphosphate (UDP)-N-acetylglucosamine-pyrophosphorylase (MsUAP), varied greatly according to normalization with the two most- and least-suited reference genes. This study will lay the basis for further molecular physiology and biochemistry studies in M. sibirica, such as development, reproduction, sex differentiation, cold and heat resistance.

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Section: Discussionmentioning

confidence: 99%

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Shen,

Tang,

et al. 2024

Front. Physiol.

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“…Among the proteins found in our analysis, there were many ribosomal proteins, which are a variety of proteins that compose the ribosome and participate in the folding of rRNA molecules into a structure that is required for the interaction of mRNA codons and tRNA anticodons ( Landry-Voyer et al., 2023 ). We excluded this class of proteins to avoid bias toward translation.…”

Section: Discussionmentioning

confidence: 99%

The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles

Dallastella,

Oliveira,

Rodrigues

et al. 2023

Front. Cell. Infect. Microbiol.

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RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species.

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“…Intriguingly, Rps2's unstructured N-terminal region seems to be a hub for the binding of multiple interaction partners (elaborated in detail in a review article by the Bachand group within this Special Issue [39]). Besides the binding partners investigated in this study (Tsr4 and importins), the N-terminal part of Rps2 also likely interacts (at least transiently) with Hmt1, as this enzyme methylates an arginine in the N-terminal region of Rps2 [40,41].…”

Section: Discussionmentioning

confidence: 99%

Dissecting the Nuclear Import of the Ribosomal Protein Rps2 (uS5)

Steiner,

Favre,

Mack

et al. 2023

Biomolecules

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The ribosome is assembled in a complex process mainly taking place in the nucleus. Consequently, newly synthesized ribosomal proteins have to travel from the cytoplasm into the nucleus, where they are incorporated into nascent ribosomal subunits. In this study, we set out to investigate the mechanism mediating nuclear import of the small subunit ribosomal protein Rps2. We demonstrate that an internal region in Rps2, ranging from amino acids 76 to 145, is sufficient to target a 3xyEGFP reporter to the nucleus. The importin-β Pse1 interacts with this Rps2 region and is involved in its import, with Rps2 residues arginine 95, arginine 97, and lysine 99 being important determinants for both Pse1 binding and nuclear localization. Moreover, our data reveal a second import mechanism involving the N-terminal region of Rps2, which depends on the presence of basic residues within amino acids 10 to 28. This Rps2 segment overlaps with the binding site of the dedicated chaperone Tsr4; however, the nuclear import of Rps2 via the internal as well as the N-terminal nuclear-targeting element does not depend on Tsr4. Taken together, our study has unveiled hitherto undescribed nuclear import signals, showcasing the versatility of the mechanisms coordinating the nuclear import of ribosomal proteins.

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Ribosomal Protein uS5 and Friends: Protein–Protein Interactions Involved in Ribosome Assembly and Beyond

Cited by 5 publications

References 100 publications

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles

Dissecting the Nuclear Import of the Ribosomal Protein Rps2 (uS5)

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