Ribosomal Protein RPL27a Promotes Female Gametophyte Development in a Dose-Dependent Manner

Zsögön, Agustín; Szakonyi, Dóra; Shi, Xiulin; Byrne, Mary E.

doi:10.1104/pp.114.241778

Cited by 33 publications

(18 citation statements)

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“…7A). This nonallelic noncomplementation genetic interaction suggests that EVR1 and EVR1L1 are functionally redundant and similar nonallelic noncomplementation interactions have been reported with mutants of other ribosomal protein coding gene families (Creff et al, 2010;Rosado et al, 2012;Casanova-Sáez et al, 2014;Zsögön et al, 2014). At F2 generation, despite extensive genotyping of F2 progenies, neither the double homozygous evr1-1 evr1l1-1 mutants nor the mutants that are homozygous at one gene and heterozygous at another gene were identified, suggesting these genotypes lead to embryonic lethality.…”

Section: Evr1 and Evr1l1 Are Functionally Redundantsupporting

confidence: 57%

Balance between Cytosolic and Chloroplast Translation Affects Leaf Variegation

et al. 2017

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The development of functional chloroplasts relies on the fine coordination of expressions of both nuclear and chloroplast genomes. We have been using the Arabidopsis () () leaf variegation mutant as a tool to dissect the regulation of chloroplast development. In this work, we screened for genetic enhancer modifiers termed () mutants and report the characterization of the first locus, We showed that encodes the cytosolic 80S ribosome 40S small subunit protein RPS21B and the loss of causes the enhancement of leaf variegation. We further demonstrated that combined S21 activities from EVR1 and its close homolog, EVR1L1, are essential for Arabidopsis, and they act redundantly in regulating leaf development and leaf variegation. Moreover, using additional cytosolic ribosomal protein mutants, we showed that although mutations in cytosolic ribosomal proteins all enhance leaf variegation to varying degrees, the 40S subunit appears to have a more profound role over the 60S subunit in regulating VAR2-mediated chloroplast development. Comprehensive genetic analyses with suppressors that are defective in chloroplast translation established that the enhancement of leaf variegation by cytosolic ribosomal protein mutants is dependent on chloroplast translation. Based on our data, we propose a model that incorporates the suppression and enhancement of leaf variegation, and hypothesize that VAR2/AtFtsH2 may be intimately involved in the balancing of cytosolic and chloroplast translation programs during chloroplast biogenesis.

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Section: Evr1 and Evr1l1 Are Functionally Redundantsupporting

confidence: 57%

Balance between Cytosolic and Chloroplast Translation Affects Leaf Variegation

et al. 2017

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“…For example, mutations in SLOW-WALKER1 (SWA1 ), which participates in 18S pre-rRNA processing, results in asynchronous megagametophyte development, and embryo sac arrest over a wide range of stages [ 62 ]. Likewise, mutations in ribosomal protein genes lead to defects in inflorescence, leaf and plant stature in Arabidopsis, similar to those observed in 35S:NTAP:AtCTF7∆B plants [ 66 - 69 ]. Therefore, many of the alterations we observe in 35S:NTAP:AtCTF7∆B plants could be the result of alterations in rRNA biogenesis or ribosome biogenesis, which in turn could indirectly impact epigenetic pathways.…”

Section: Discussionmentioning

confidence: 54%

“…For example, previous studies have shown that eco1/ctf7 mutations result in defects in nucleolar integrity, rRNA production, ribosome biogenesis and protein biosynthesis in Saccharomyces cerevisiae and human [ 25 , 61 ]. In Arabidopsis, mutations in genes participating in mRNA production and rRNA/ribosome biogenesis slow mitotic progression in female gametophytes and result in pleiotropic defects in embryo sacs [ 58 , 59 , 62 - 66 ]. For example, mutations in SLOW-WALKER1 (SWA1 ), which participates in 18S pre-rRNA processing, results in asynchronous megagametophyte development, and embryo sac arrest over a wide range of stages [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a truncated CTF7 construct leads to pleiotropic defects in reproduction and vegetative growth in Arabidopsis

Liu

Makaroff

2015

BMC Plant Biol

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BackgroundEco1/Ctf7 is essential for the establishment of sister chromatid cohesion during S phase of the cell cycle. Inactivation of Ctf7/Eco1 leads to a lethal phenotype in most organisms. Altering Eco1/Ctf7 levels or point mutations in the gene can lead to alterations in nuclear division as well as a wide range of developmental defects. Inactivation of Arabidopsis CTF7 (AtCTF7) results in severe defects in reproduction and vegetative growth.ResultsTo further investigate the function(s) of AtCTF7, a tagged version of AtCTF7 and several AtCTF7 deletion constructs were created and transformed into wild type or ctf7+/–plants. Transgenic plants expressing 35S:NTAP:AtCTF7∆299–345 (AtCTF7∆B) displayed a wide range of phenotypic alterations in reproduction and vegetative growth. Male meiocytes exhibited chromosome fragmentation and uneven chromosome segregation. Mutant ovules contained abnormal megasporocyte-like cells during pre-meiosis, megaspores experienced elongated meiosis and megagametogenesis, and defective megaspores/embryo sacs were produced at various stages. The transgenic plants also exhibited a broad range of vegetative defects, including meristem disruption and dwarfism that were inherited in a non-Mendelian fashion. Transcripts for epigenetically regulated transposable elements (TEs) were elevated in transgenic plants. Transgenic plants expressing 35S:AtCTF7∆B displayed similar vegetative defects, suggesting the defects in 35S:NTAP:AtCTF7∆B plants are caused by high-level expression of AtCTF7∆B.ConclusionsHigh level expression of AtCTF7∆B disrupts megasporogenesis, megagametogenesis and male meiosis, as well as causing a broad range of vegetative defects, including dwarfism that are inherited in a non-Mendelian fashion.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0452-2) contains supplementary material, which is available to authorized users.

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“…Many previous studies have indicated that a mutation of a specific chloroplast ribosomal protein could affect plant growth, development, and metabolism, including embryogenesis, leaf patterning, female gametophyte development, seed germination, seedling development, and photosynthesis (Pesaresi et al, 2001 ; Morita-Yamamuro et al, 2004 ; Bryant et al, 2011 ; Szakonyi and Byrne, 2011 ; Romani et al, 2012 ; Gong et al, 2013 ; Zsögön et al, 2014 ). In most of these cases, the research demonstrated that chloroplast translation was impaired in the mutants.…”

Section: Discussionmentioning

confidence: 99%

Functional Disruption of a Chloroplast Pseudouridine Synthase Desensitizes Arabidopsis Plants to Phosphate Starvation

Zhang

et al. 2017

Front. Plant Sci.

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Phosphate (Pi) deficiency is a common nutritional stress of plants in both agricultural and natural ecosystems. Plants respond to Pi starvation in the environment by triggering a suite of biochemical, physiological, and developmental changes that increase survival and growth. The key factors that determine plant sensitivity to Pi starvation, however, are unclear. In this research, we identified an Arabidopsis mutant, dps1, with greatly reduced sensitivity to Pi starvation. The dps1 phenotypes are caused by a mutation in the previously characterized SVR1 (SUPPRESSION OF VARIAGATION 1) gene, which encodes a chloroplast-localized pseudouridine synthase. The mutation of SVR1 results in defects in chloroplast rRNA biogenesis, which subsequently reduces chloroplast translation. Another mutant, rps5, which contains a mutation in the chloroplast ribosomal protein RPS5 and has reduced chloroplast translation, also displayed decreased sensitivity to Pi starvation. Furthermore, wild type plants treated with lincomycin, a chemical inhibitor of chloroplast translation, showed similar growth phenotypes and Pi starvation responses as dps1 and rps5. These results suggest that impaired chloroplast translation desensitizes plants to Pi starvation. Combined with previously published results showing that enhanced leaf photosynthesis augments plant responses to Pi starvation, we propose that the decrease in responses to Pi starvation in dps1, rps5, and lincomycin-treated plants is due to their reduced demand for Pi input from the environment.

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Ribosomal Protein RPL27a Promotes Female Gametophyte Development in a Dose-Dependent Manner

Cited by 33 publications

References 70 publications

Balance between Cytosolic and Chloroplast Translation Affects Leaf Variegation

Balance between Cytosolic and Chloroplast Translation Affects Leaf Variegation

Overexpression of a truncated CTF7 construct leads to pleiotropic defects in reproduction and vegetative growth in Arabidopsis

Functional Disruption of a Chloroplast Pseudouridine Synthase Desensitizes Arabidopsis Plants to Phosphate Starvation

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