Ribosomal protein NtRPL17 interacts with kinesin-12 family protein NtKRP and functions in the regulation of embryo/seed size and radicle growth

Tian, Shujuan; Wu, Jingjing; Liu, Yuan; Huang, Xiaorong; Li, Fen; Wang, Zhaodan; Sun, Mingwei

doi:10.1093/jxb/erx361

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…This is the case of a Glycyl-tRNA synthetase, present in both tissues, whose gene inactivation in Arabidopsis thaliana led to plant embryo development arrest [34]. Ribosomal proteins and elongation factors have also been implicated in seed development and dormancy in N. tabacum and A. thaliana [35]. The evolution pattern of these proteins is similar to that reported for other groups in cotyledons, with the maximum at M6-M8.…”

Section: Discussionsupporting

confidence: 54%

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Sghaier-Hammami

Hammami

Baazaoui

et al. 2020

IJMS

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Unlike orthodox species, seed recalcitrance is poorly understood, especially at the molecular level. In this regard, seed maturation and germination were studied in the non-orthodox Quercus ilex by using a proteomics strategy based on two-dimensional gel electrophoresis coupled to matrix-assisted laser desorption ionization/time of flight (2-DE-MALDI-TOF).Cotyledons and embryo/radicle were sampled at different developmental stages, including early (M1–M3), middle (M4–M7), and late (M8–M9) seed maturation, and early (G1–G3) and late (G4–G5) germination. Samples corresponding to non-germinating, inviable, seeds were also included. Protein extracts were subjected to 2-dimensional gel electrophoresis (2-DE) and changes in the protein profiles were analyzed. Identified variable proteins were grouped according to their function, being the energy, carbohydrate, lipid, and amino acid metabolisms, together with protein fate, redox homeostasis, and response to stress are the most represented groups. Beyond the visual aspect, morphometry, weight, and water content, each stage had a specific protein signature. Clear tendencies for the different protein groups throughout the maturation and germination stages were observed for, respectively, cotyledon and the embryo axis. Proteins related to metabolism, translation, legumins, proteases, proteasome, and those stress related were less abundant in non-germinating seeds, it related to the loss of viability. Cotyledons were enriched with reserve proteins and protein-degrading enzymes, while the embryo axis was enriched with proteins of cell defense and rescue, including heat-shock proteins (HSPs) and antioxidants. The peaks of enzyme proteins occurred at the middle stages (M6–M7) in cotyledons and at late ones (M8–M9) in the embryo axis. Unlike orthodox seeds, proteins associated with glycolysis, tricarboxylic acid cycle, carbohydrate, amino acid and lipid metabolism are present at high levels in the mature seed and were maintained throughout the germination stages. The lack of desiccation tolerance in Q. ilex seeds may be associated with the repression of some genes, late embryogenesis abundant proteins being one of the candidates.

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Section: Discussionsupporting

confidence: 54%

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Sghaier-Hammami

Hammami

Baazaoui

et al. 2020

IJMS

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“…Although, the functional relationship depicted in the co-expression network did not include all the 36 candidate genes predicted in this study, it gives better clues about 15 genes out of the predicted genes together with other genes which were not captured in this study. There is intensive literature on the interaction of ribosomal proteins and kinesin proteins in regulating embryo/seed size and radicle growth [ 80 ]. Such interaction was evident in the network constructed in this study.…”

Section: Discussionmentioning

confidence: 99%

Identification of quantitative trait nucleotides and candidate genes for soybean seed weight by multiple models of genome-wide association study

et al. 2020

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Background Seed weight is a complex yield-related trait with a lot of quantitative trait loci (QTL) reported through linkage mapping studies. Integration of QTL from linkage mapping into breeding program is challenging due to numerous limitations, therefore, Genome-wide association study (GWAS) provides more precise location of QTL due to higher resolution and diverse genetic diversity in un-related individuals. Results The present study utilized 573 breeding lines population with 61,166 single nucleotide polymorphisms (SNPs) to identify quantitative trait nucleotides (QTNs) and candidate genes for seed weight in Chinese summer-sowing soybean. GWAS was conducted with two single-locus models (SLMs) and six multi-locus models (MLMs). Thirty-nine SNPs were detected by the two SLMs while 209 SNPs were detected by the six MLMs. In all, two hundred and thirty-one QTNs were found to be associated with seed weight in YHSBLP with various effects. Out of these, seventy SNPs were concurrently detected by both SLMs and MLMs on 8 chromosomes. Ninety-four QTNs co-localized with previously reported QTL/QTN by linkage/association mapping studies. A total of 36 candidate genes were predicted. Out of these candidate genes, four hub genes ( Glyma06g44510, Glyma08g06420, Glyma12g33280 and Glyma19g28070 ) were identified by the integration of co-expression network. Among them, three were relatively expressed higher in the high HSW genotypes at R5 stage compared with low HSW genotypes except Glyma12g33280 . Our results show that using more models especially MLMs are effective to find important QTNs, and the identified HSW QTNs/genes could be utilized in molecular breeding work for soybean seed weight and yield. Conclusion Application of two single-locus plus six multi-locus models of GWAS identified 231 QTNs. Four hub genes ( Glyma06g44510 , Glyma08g06420 , Glyma12g33280 & Glyma19g28070 ) detected via integration of co-expression network among the predicted candidate genes.

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“…Existing literature shows that ribosomal proteins are involved in various physiological functions of cells and have been reported to regulate plant growth [ 41 ]. For example, ribosomal protein NtRPL17 interacts with kinesin-12 family protein NtKRP and functions in the regulation of embryo/seed size and radicle growth [ 49 ], cytoplasmic ribosomal protein L14B is essential for fertilization in Arabidopsis [ 50 ], Dek44Maize encodes mitochondrial Ribosomal Protein L9 and is required for seed development [ 51 ], the essential chloroplast ribosomal protein uL15c interacts with the chloroplast RNA helicase ISE2 and affects intercellular trafficking through plasmodesmata [ 52 ], down-regulation of specific plastid ribosomal proteins suppresses thf1 leaf variegation, implying a role of THF1 in plastid gene expression [ 53 ], Salicylic acid-related cotton ( G. arboreum ) ribosomal protein GaRPL18 contributes to resistance to V. dahliae [ 16 ]. TOR and RPS6 enhance protein translation in A. thaliana seedlings by transmitting light signals [ 54 ], ribosomal protein S6 may be involved in the regulation of rRNA genes through epigenetic changes in A. thaliana [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

Zhu

Zhang

Zhou

et al. 2021

IJMS

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Verticillium wilt is threatening the world’s cotton production. The pathogenic fungus Verticillium dahliae can survive in the soil in the form of microsclerotia for a long time, colonize through the root of cotton, and invade into vascular bundles, causing yellowing and wilting of cotton leaves, and in serious cases, leading to plant death. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. In previous studies, proteomic analysis was carried out on different cotton varieties inoculated with V. dahliae strain Vd080. It was found that GhRPS6 was phosphorylated after inoculation, and the phosphorylation level in resistant cultivars was 1.5 times than that in susceptible cultivars. In this study, knockdown of GhRPS6 expression results in the reduction of SA and JA content, and suppresses a series of defensive response, enhancing cotton plants susceptibility to V. dahliae. Overexpression in Arabidopsis thaliana transgenic plants was found to be more resistant to V. dahliae. Further, serines at 237 and 240 were mutated to phenylalanine, respectively and jointly. The transgenic Arabidopsis plants demonstrated that seri-237 compromised the plant resistance to V. dahliae. Subcellular localization in Nicotiana benthamiana showed that GhRPS6 was localized in the nucleus. Additionally, the pathogen inoculation and phosphorylation site mutation did not change its localization. These results indicate that GhRPS6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.

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Ribosomal protein NtRPL17 interacts with kinesin-12 family protein NtKRP and functions in the regulation of embryo/seed size and radicle growth

Abstract: Direct interaction between NtRPL17 and the kinesin NtKRP controls cell cycle progression in plants and reveals a novel regulatory role for ribosomal proteins in seed size establishment.

Cited by 12 publications

References 41 publications

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Dissecting the Seed Maturation and Germination Processes in the Non-Orthodox Quercus ilex Species Based on Protein Signatures as Revealed by 2-DE Coupled to MALDI-TOF/TOF Proteomics Strategy

Identification of quantitative trait nucleotides and candidate genes for soybean seed weight by multiple models of genome-wide association study

Genome-Wide Analysis of Ribosomal Protein GhRPS6 and Its Role in Cotton Verticillium Wilt Resistance

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