Ribosomal DNA spacer-length variation inSecale spp. (Poaceae)

Reddy, P.V.G.K.; Appels, R.; Baum, Bernard R.

doi:10.1007/bf00940606

Cited by 30 publications

(19 citation statements)

References 26 publications

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“…Our study showed that the S. sylvestre accessions were very divergent from the accessions of the other species investigated, agreeing with the majority of previous studies (Reddy et al 1990, Del Pozo et al 1995, De Bustos and Jouve 2002. Our S. strictum accessions were heterogeneous, with large differences between subspecies.…”

Section: Discussionsupporting

confidence: 93%

“…The phylogenetic relationships of Secale at the species level has been investigated using diverse techniques (Venses et al 1987a, Reddy et al 1990, Del Pozo et al 1995, De Bustos and Jouve 2002. Our study showed that the S. sylvestre accessions were very divergent from the accessions of the other species investigated, agreeing with the majority of previous studies (Reddy et al 1990, Del Pozo et al 1995, De Bustos and Jouve 2002.…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers

et al. 2006

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The genetic diversity and phylogenetic relationships in the genus Secale L. (rye) was evaluated using 24 Secale cereale microsatellite (SCM) markers. The average polymorphism information content (PIC) value of each microsatellite locus in 30 Secale accessions evaluated was higher than that in 47 cultivated ryes (Secale cereale ssp. cereale). The mean genetic similarity (GS) index in Secale was lower than that in cultivated rye. The highest within-species GS index was observed for S. sylvestre and the lowest for S. strictum, whereas the highest between-species GS index was found between S. cereale and S. vavilovii and the lowest between S. sylvestre and S. cereale. There was no obvious difference in GS levels in the cultivated rye accessions from Asia, Europe, North America or South America. Cluster analysis indicated that all the Secale accessions could be distinguished by the 24 microsatellite loci. We also found that the S. sylvestre accessions were obviously divergent from the accessions of other species and that the S. vavilovii accessions were closely related to the S. cereale accessions. Our results also showed that S. strictum was heterogeneous and showed great within-species differences. The microsatellite-derived dendrogram faithfully reflected the phylogenetic relationships between Secale species but did not indicate a possible domestication process of the cultivated rye based on the geographical sources of the accessions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers

et al. 2006

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“…Secale cereale has been studied by morphological (Frederiksen and Petersen 1998;Persson et al 2006), cytological (Gill and Kimber 1974;Schlegel et al 1987;Alkimova et al 2004), isozymes (Vence et al 1987), ribosomal DNA spacer lengths (Reddy et al 1990), restriction fragment length polymorphisms (RFLPs) (Saal and Wricke 1999), amplified fragment length polymorphisms (AFLPs) (Chikmawati et al 2005) and microsatellite (Shang et al 2006;Saal and Wricke 1999;Bolibok et al 2005) analyses. Bolibok et al (2005) used Genomic-SSR, EST-SSR, ISSR and SAMPL markers in assessing genetic diversity of S. cereale.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Secale cereale L. in Iran as measured using microsatellites

Akhavan

Saeidi

Rahiminejad

2009

Genet Resour Crop Evol

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Microsatellite markers were used to analyse the genetic diversity of 120 individuals of 20 weedy/wild populations of Secale cereale collected all around Iran. High levels of diversity, with an average number of seven alleles per locus (ranging up to 11) and high level polymorphism with polymorphism rate averaging 0.645 (between populations) and 0.364 (within populations) were observed. The Northwestern populations showed the highest and the Northern populations showed the lowest polymorphism and diversity. One population was notably more similar to five of the S. strictum used as outgroups. No taxon or geographic specific marker was detected, suggesting high gene flow between the populations, however some groupings which can be related to the subspecies, were evident. This survey showed the high genetic diversity within the Iranian rye genepool as an important source for crop breeders, and indicated that there is value in sampling for useful genes for crops improvement.

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“…An aspect of the diversity of the 5S DNA gene within and among species was investigated using the nontranscribed spacer (NTS) length (Reddy et al 1990). In a first attempt to find the relationship among selected genera of the Triticeae using sequences of the 5S DNA units we found that the NTS can be clearly divided into two types, viz.…”

Section: Introductionmentioning

confidence: 99%

The 5S rRNA gene diversity in the genus Secale and determination of its closest haplomes

Shang

Baum

Wei

et al. 2007

Genet Resour Crop Evol

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We analyzed 127 rDNA sequences (5S DNA units) obtained from 23 seed accession samples from more or less 10 taxa in wild and cultivated rye, genus Secale L. The sequences fell into two known groups, here assigned to two unit classes, viz. long R1 and short R1 (designations to reflect on R haplome of rye). The different taxa could not be fully differentiated based on the 5S DNA units. We searched for 5S DNA sequences from known unit classes most closely similar to the long R1 and the short R1. One set with the long R1 unit class contained sequences of the long P1 unit class from Agropyron (P haplome) and from Kengyilia (StYP haplome), long J1 from Thinopyrum (J haplome), whereas the set with the short R1 included the long S1 from Pseudoroegneria (St haplome) and Kengyilia (StYP haplome), the short J1 from Thinopyrum (J haplome) and the short V1 from Dasypyrum (V haplome). Each of the two sets was analyzed separately by maximum likelihood (ML) phylogenetic analysis from which we were able to infer that the 5S DNA units of Secale differentiated in a non-clock fashion and followed the HKY substitution model in the gene tree with the long R1 unit class and the HKY + G in the gene tree with the short R1 unit class. A complementary Bayesian analysis yielded identical tree topologies to the ML ones for each of the two sequence sets. In the tree with the long R1 units the long P1 and long J1 unit classes were closest to the long R1 unit class, whereas in the tree with the short R1 units the long S1 and short J1 unit classes were closest to the short R1 unit class, indicating possibly a close relationship between the St, J and R haplomes.

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Ribosomal DNA spacer-length variation inSecale spp. (Poaceae)

Cited by 30 publications

References 26 publications

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers

Genetic diversity of Secale cereale L. in Iran as measured using microsatellites

The 5S rRNA gene diversity in the genus Secale and determination of its closest haplomes

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