Ribosomal DNA Sequence Analysis of Mucosa-Associated Bacteria in Crohn’s Disease

Prindiville, Thomas P; Cantrell, Mary C.; Wilson, Kenneth H.

doi:10.1097/00054725-200411000-00017

Cited by 84 publications

(52 citation statements)

References 20 publications

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“…However, conflicting reports do exist where levels of Bacteroides and lactobacilli showed no difference between Crohn's disease samples and control samples, while the bifidobacteria were significantly decreased in numbers (13). Furthermore, culture-independent investigations of the dynamics of the intestinal bacterial community in Crohn's disease have either concentrated on the dominant species (24,44,45,52) or quantified the species in specific groups (53,58), and each analysis has used only a single-timepoint sample with no temporal data presented. The consensus of opinion from these investigations is that there is a reduction in the diversity of the dominant bacterial species in Crohn's disease.…”

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confidence: 99%

Culture-Independent Analyses of Temporal Variation of the Dominant Fecal Microbiota and Targeted Bacterial Subgroups in Crohn's Disease

et al. 2006

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Gut microbiota shows host-specific diversity and temporal stability and significantly contributes to maintenance of a healthy gut. However, in inflammatory bowel disease, this microbiota has been implicated as a contributory factor to the illness. This study compared bacterial dynamics in Crohn's disease patients to those in a control group using a culture-independent method to assess the temporal stability, relative diversity, and similarity of the dominant fecal microbiota, Clostridium spp., Bacteroides spp., Bifidobacterium spp., and lactic acid bacteria spp. (LAB) for all individuals. Fecal samples were collected over several time points from individuals with Crohn's disease who were in remission (n ‫؍‬ 11), from Crohn's disease patients who relapsed into an active Crohn's disease state (n ‫؍‬ 5), and from a control group (n ‫؍‬ 18). Denaturing gradient gel electrophoresis profiles were generated for the different microbial groups by specifically targeting different regions of the 16S rRNA gene and were compared on the basis of similarity and diversity. The temporal stability of dominant species for all Crohn's disease patients was significantly lower (P < 0.005) than that for the control group. Analysis of group-specific profiles for Bifidobacterium spp. found that they were similar in all samples, while the diversity of the LAB varied significantly between the groups, but temporal stability was not significantly altered. We observed significant changes in two functionally important mutualistic groups of bacteria, viz., Clostridium and Bacteroides spp., which may have implications for the host's gut health, since some genera are involved in production of short-chain fatty acid, e.g., butyrate.

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confidence: 99%

Culture-Independent Analyses of Temporal Variation of the Dominant Fecal Microbiota and Targeted Bacterial Subgroups in Crohn's Disease

et al. 2006

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“…These studies were also inconsistent in that one group reported an overrepresentation of the phylum Bacteriodetes in Crohn disease and ulcerative colitis tissue, while another group showed no specific bacterial phylum associated with diseased or control tissue. Overall, any reported differences in phylogenetic groups between patients and controls were not sufficiently reproducible to justify the conclusion that organisms of any one group was associated with IBD (43).…”

Section: The Case For the Presence Of Abnormal Microfloramentioning

confidence: 87%

“…Complementary studies using molecular techniques to identify specific bacterial groups have also been applied to the study of the bacterial microflora in IBD (42)(43)(44). These studies do not support the presence of a specific, pathogenic organism in IBD, in that profiles of mucosa-associated bacterial ribosomal DNA showed no difference between inflamed and uninvolved areas.…”

Section: The Case For the Presence Of Abnormal Microfloramentioning

confidence: 99%

The fundamental basis of inflammatory bowel disease

Strober¹,

Fuss²,

Mannon³

2007

J. Clin. Invest.

1,192

923

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“…Indeed, recent studies demonstrate that obesity in humans and ob/ob mice is associated with stereotypical imbalances in the normal gut microbiota (31)(32)(33). Likewise, previous studies of human IBD, using standard culture techniques (34)(35)(36) or molecular analysis (37)(38)(39)(40)(41)(42)(43), have noted alterations in the GI microbiota. However, most of these IBD studies have been limited in statistical power and precision of identification or have examined only the fecal microbiota, which differs substantially from that of the GI mucosa (44).…”

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confidence: 96%

Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases

Frank

Amand

Feldman

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

3,930

193

2,968

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The two primary human inflammatory bowel diseases, Crohn's disease (CD) and ulcerative colitis (UC), are idiopathic relapsing disorders characterized by chronic inflammation of the intestinal tract. Although several lines of reasoning suggest that gastrointestinal (GI) microbes influence inflammatory bowel disease (IBD) pathogenesis, the types of microbes involved have not been adequately described. Here we report the results of a cultureindependent rRNA sequence analysis of GI tissue samples obtained from CD and UC patients, as well as non-IBD controls. Specimens were obtained through surgery from a variety of intestinal sites and included both pathologically normal and abnormal states. Our results provide comprehensive molecular-based analysis of the microbiota of the human small intestine. Comparison of clone libraries reveals statistically significant differences between the microbiotas of CD and UC patients and those of non-IBD controls. Significantly, our results indicate that a subset of CD and UC samples contained abnormal GI microbiotas, characterized by depletion of commensal bacteria, notably members of the phyla Firmicutes and Bacteroidetes. Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances.Crohn's disease ͉ culture-independent microbiology ͉ ulcerative colitis ͉ rRNA

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Ribosomal DNA Sequence Analysis of Mucosa-Associated Bacteria in Crohn’s Disease

Abstract: These findings suggest that CD is not caused by invasive pathogens associated specifically with the sites of lesions but that dysbiosis exists in this condition.

Cited by 84 publications

References 20 publications

Culture-Independent Analyses of Temporal Variation of the Dominant Fecal Microbiota and Targeted Bacterial Subgroups in Crohn's Disease

Culture-Independent Analyses of Temporal Variation of the Dominant Fecal Microbiota and Targeted Bacterial Subgroups in Crohn's Disease

The fundamental basis of inflammatory bowel disease

Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases

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