Ribosomal DNA plasmid isolated from Zygosaccharomyces bailii and its use for constructing yeast vectors effective for intergeneric gene transfer.

Sugihara, K.; Ohishi, Kazuhiko; Toh‐e, Akio

doi:10.1271/bbb1961.50.1503

Cited by 6 publications

(2 citation statements)

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“…Southern hybridizations were performed using an rDNA probe which was prepared from a 10.8 kb SaZI fragment of pAT68 which contains a rDNA repeat unit (Sugihara et al, 1986). After the fragment was cut with KpnI, the probe was labelled by a random priming method with 32P as described previously (Asakura et al, 1991) or with Digoxigenin using a kit (Boehringer Mannheim).…”

Section: Methodsmentioning

confidence: 99%

Clonal size-variation of rDNA cluster region on chromosome XII of Sacchavomyces cerevisiae

Chindamporn

Iwaguchi²,

Nakagawa³

et al. 1993

Journal of General Microbiology

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Using pulsed-field gel electrophoresis (PFGE), we have demonstrated clonal variation in the size of chromosome XI1 in a diploid strain of Saccharomyces cerevisiae X2180-2D. The sizes of the two chromosome XII homologues were very different : 2600 (L-type) and 1450 kb (S-type). The frequency with which we detected clonal size variation in the diploid, compared to that of the parental clones, was about 1540% of the progeny clones and the range of the size variation of the homologues was 2580-2680 kb (L-type) and 1340-1500 kb (S-type), respectively. The homologue of the L-type appeared to be more frequently variable than that of the S-type. The size variation was shown to be derived from size changes in the rDNA cluster region, which is present in chromosome XII, by digesting the chromosome with XhoI, whose cutting site is not present in a rDNA repeat unit, and hybridizing to rDNA probes. The clonal size variation was also investigated in haploids from spores after meiosis. The L-type and Stype chromosomes segregated 2:2 in an ascus and the sizes of all the S-type chromosomes were shifted up, compared to the original diploid, though the L-type ones were stable. The S-type sizes of 1340,1450 and 1780 kb in the original diploids changed into the ranges of 1475-1610 kb, 1520-1680 kb and 1820-2010 kb, respectively, in the segregants. Furthermore, we observed that the size of S-type chromosomes in haploid cells was gradually increasing in mitosis during successive subcultures. The rDNA units appeared to be amplified on the S-type chromosome.

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Section: Methodsmentioning

confidence: 99%

Clonal size-variation of rDNA cluster region on chromosome XII of Sacchavomyces cerevisiae

Chindamporn

Iwaguchi²,

Nakagawa³

et al. 1993

Journal of General Microbiology

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“…Southern hybridization. Hybridization with rDNA probe (15) was done according to the method described by Iwaguchi et al (5).…”

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confidence: 99%

Electrophoretic Karyotypes of Candida Yeasts Recurrently Isolated from Single Patients

Doi

Mizuguchi

Homma

et al. 1994

Microbiology and Immunology

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Candida strains were isolated repeatedly from single patients during recurrent episodes of Candida infection in a hospital, and their electrophoretic karyotypes were analyzed by pulsedfield gel electrophoresis using CHEF system. When only C. albicans (in 6 patients) or C. glabrata (in 1 patient) were recurrently isolated, their karyotypes from each patient were almost identical to one another, suggesting that they carried single type of the yeast. When multiple species were recovered from single patients (6 cases), the karyotypes of the most frequently recovered yeast species were almost identical with respect to each patient. The electrophoretic karyotype analysis has been proved to be useful for epidemiological studies because the method can tell not only the species identification but also the differences among the strains of the same species.

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Primers for amplification of mt SSU rDNA, and a phylogenetic study of Botryosphaeria and associated anamorphic fungi

Zhou

Stanosz

2001

Mycological Research

128

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Ribosomal DNA plasmid isolated from Zygosaccharomyces bailii and its use for constructing yeast vectors effective for intergeneric gene transfer.

Cited by 6 publications

References 1 publication

Clonal size-variation of rDNA cluster region on chromosome XII of Sacchavomyces cerevisiae

Clonal size-variation of rDNA cluster region on chromosome XII of Sacchavomyces cerevisiae

Electrophoretic Karyotypes of Candida Yeasts Recurrently Isolated from Single Patients

Primers for amplification of mt SSU rDNA, and a phylogenetic study of Botryosphaeria and associated anamorphic fungi

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