1986
DOI: 10.1271/bbb1961.50.1503
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Ribosomal DNA plasmid isolated from Zygosaccharomyces bailii and its use for constructing yeast vectors effective for intergeneric gene transfer.

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Cited by 6 publications
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“…Southern hybridizations were performed using an rDNA probe which was prepared from a 10.8 kb SaZI fragment of pAT68 which contains a rDNA repeat unit (Sugihara et al, 1986). After the fragment was cut with KpnI, the probe was labelled by a random priming method with 32P as described previously (Asakura et al, 1991) or with Digoxigenin using a kit (Boehringer Mannheim).…”
Section: Methodsmentioning
confidence: 99%
“…Southern hybridizations were performed using an rDNA probe which was prepared from a 10.8 kb SaZI fragment of pAT68 which contains a rDNA repeat unit (Sugihara et al, 1986). After the fragment was cut with KpnI, the probe was labelled by a random priming method with 32P as described previously (Asakura et al, 1991) or with Digoxigenin using a kit (Boehringer Mannheim).…”
Section: Methodsmentioning
confidence: 99%
“…Southern hybridization. Hybridization with rDNA probe (15) was done according to the method described by Iwaguchi et al (5).…”
mentioning
confidence: 99%