Ribosomal DNA is located and transcribed in the dense fibrillar component of human Sertoli cell nucleoli

Wachtler, F.; Hartung, M; Devictor, M.; Wiegant, J.; Stahl, A; Schwarzacher, H. G.

doi:10.1016/0014-4827(89)90364-9

Cited by 67 publications

(26 citation statements)

References 32 publications

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“…Based on in situ hybridization with a radioactive probe, Arroua et al (1982) reported that, in segregated nucleoli of human spermatocytes at late pachytene, the distribution of the silver grains corresponded to the position of the fibrillar centers. On the contrary, in human Sertoli cell nucleoli, which also display a segregated arrangement of their components, the pattern of in situ hybridization of a biotinylated probe to rDNA was taken as evidence that the distribution of rDNA corresponded to the dense fibrillar component rather than to the fibrillar centers (Wachtler et al, 1989). In a recent study, Rawlins and Shaw (1990) used a biotinylated cDNA probe to describe the three-dimensional organization of rDNA in nucleoli from pea root tips.…”

Section: Rdna Localization By In Situ Hybridizationmentioning

confidence: 99%

“…However, the precise relationships between these structural components and their molecular functions remain unresolved. In particular, the spatial distribution of the transcribing rRNA genes within the nucleolar body is still a matter of debate (compare, e.g., the results and conceptions presented by Scheer andRose, 1984, andWachtler et al, 1989). In order to understand the functional organization of the nucleolus, it is of fundamental importance to know where exactly the active rRNA genes or transcription units are located.…”

Section: Introductionmentioning

confidence: 99%

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Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Thiry

Scheer

Goessens

1991

Electron Microscopy Reviews

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Abstract-Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure.Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA to po isomerase I are located together.Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.

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Section: Rdna Localization By In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Thiry

Scheer

Goessens

1991

Electron Microscopy Reviews

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“…In situ hybridization with both the TU and NTS probes reveals padlock-shaped fluo rescent areas. The dark round area seen at the periphery of the nucleoli corresponds to the fibrillar centers (Wachtler et al, 1989). The position of the label corresponds to that of the dense fibrillar material and is superimposable for both the TUs and NTS sequences (Fig.…”

Section: Sertoli Cellsmentioning

confidence: 99%

“…1987: Wachtler et al. 1989: Within the single nucleolus one large fibrillar center is located at the periphery of the nucleo lus and is surrounded by dense fibrillar material.…”

Section: Sertoli Cellsmentioning

confidence: 99%

“…The transcription units (TUs), present in multiple copies, are interspersed with regions of non transcribed spacer (NTS) sequences, now called " intergenic regions '' (Sylvester et al, 1986). The exact arrangement of these DNA sequences within the nucleolus is not clear: Although both the transcribed and nontranscribed parts appear in recent stud ies to be localized to the dense fibrillar component of the nucleo lus (Wachtler et al, 1989(Wachtler et al, , 1990a, and unpublished observa tions), it has been suggested that they may, in fact, be located in different nucleolar components or at different sites within the nucleolus (Schwarzacher and Wachtler, 1987; Marilley and Gassend-Bonnet. 1989).…”

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Transcribed and nontranscribed parts of the human ribosomal gene repeat show a similar pattern of distribution in nucleoli

Wachtler

Schöfer

Schedle

et al. 1991

Cytogenet Genome Res

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The distribution pattern of the transcribed and nontranscribed parts of human ribosomal RNA genes were visualized simultaneously in the same cells by nonautoradiographic in situ hybridization. DNA probes labeled with either digoxigenin or biotin were detected in the same cells by different fluorescence systems. The signals from both the transcribed and nontranscribed parts showed a similar distribution pattern. This finding is not compatible with the conclusion, suggested by earlier studies, that the transcribed and nontranscribed parts of the rRNA genes are located at different sites within the nucleoli or in different nucleolar components.

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Ultrastructural and morphometric analysis of nuclear and nuclear changes during the early growth period in hamster facial neurons

Clark

Jones

LaVelle

1990

J of Comparative Neurology

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In this study, progressive developmental changes in the nucleus and associated organelles, including the nucleolus, coiled bodies, nuclear envelope, and nucleoplasm, of hamster facial motor neurons were characterized by two parallel analyses: ultrastructural and morphometric. Golden hamsters (Mesocricetus auratus) used for this series were the 14-day fetus, newborn (less than 6 hr), and 1, 2, 3, 4, 5, 7, 9, 11 and 13 days postnatal ages, with 3 animals per group. Following anesthesia and perfusion fixation, facial nuclear groups were dissected and processed for electron microscopy. Electron micrographs and camera lucida tracings of nuclear profiles were collected and analyzed. The ultrastructural analysis revealed progressive changes in the nucleolus from a compact, segregated type to a reticulated form characteristic of actively protein-secreting cells. Nucleolar microbodies and fibrillar centers were seen at all ages; the latter structures appeared to decrease in size and increase with age in the series. The nucleolus-associated chromatin became less condensed, suggesting an increase in the incorporation of rDNA into the nucleolus proper. Coiled bodies, both free and attached to nucleoli, were found in varying frequencies. The nucleoplasm of neurons at the earliest stages contained large numbers of heterochromatin clumps, which decreased concomitantly with an increase in interchromatin granules and fibrils during the later stages. Nuclear envelope invaginations, polarized along one side of the nucleus, increased throughout the developmental period examined. These changes occurred in concert with a 61% increase in nuclear size and a 47% increase in the length of nuclear envelope. The sequence of nuclear changes observed during this early period of normal facial neuronal growth completes the study of a series of distinctly defined cytomorphic events in this cell type, the lability of which can be experimentally tested for their functional roles in neuronal development.

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Ribosomal DNA is located and transcribed in the dense fibrillar component of human Sertoli cell nucleoli

Cited by 67 publications

References 32 publications

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level

Transcribed and nontranscribed parts of the human ribosomal gene repeat show a similar pattern of distribution in nucleoli

Ultrastructural and morphometric analysis of nuclear and nuclear changes during the early growth period in hamster facial neurons

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