Ribophorin II promotes cell proliferation, migration, and invasion in esophageal cancer cells in vitro and in vivo

Li, Yongshun; Huang, Changrong; Bai, Qizhou; Yu, Jin

doi:10.1042/bsr20182448

Cited by 8 publications

(8 citation statements)

References 25 publications

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“…Next, targets of miR-378e were explored and here we first confirmed RPN2 as a functional target of miR-378e in glioma. Accruing studies suggested RPN2 as an oncogene in multiple cancers, including breast cancer, colon carcinoma, nasopharyngeal carcinoma and esophageal cancer [30][31][32][33]. Hence, we hypothesized that RPN2 might be also as a carcinogenic gene in glioma.…”

Section: Discussionmentioning

confidence: 95%

CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Ding

You

et al. 2019

J Exp Clin Cancer Res

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Background: Circular RNA nuclear factor I X (circNFIX) has been reported to play an important role in glioma progression. However, the mechanism by which circNFIX participates in glioma progression remains poorly understood.Methods: GERIA online were used to analyze the abnormally expressed genes in glioma tissues. The expression levels of circNFIX, microRNA (miR)-378e and Ribophorin-II (RPN2) were measured by quantitative real-time polymerase chain reaction or western blot. Cell cycle distribution, apoptosis, glycolysis, migration and invasion were determined by flow cytometry, special kit and trans-well assays, respectively. The target association between miR-378e and circNFIX or RPN2 was confirmed by luciferase reporter assay, RNA immunoprecipitation and pull-down. Xenograft model was established to investigate the role of circNFIX in vivo.Results: The expression of circNFIX was enhanced in glioma tissues and cells compared with matched controls and high expression of circNFIX indicated poor outcomes of patients. Knockdown of circNFIX led to arrest of cell cycle, inhibition of glycolysis, migration and invasion and promotion of apoptosis in glioma cells. circNFIX was a sponge of miR-378e. miR-378e overexpression suppressed cell cycle process, glycolysis, migration and invasion but promoted apoptosis. miR-378e silence abated the suppressive role of circNFIX knockdown in glioma progression. RPN2 as a target of miR-378e was positively regulated via circNFIX by competitively sponging miR-378e. Silencing circNFIX decreased glioma xenograft tumor growth by regulating miR-378e/RPN2 axis. Conclusion: Knockdown of circNFIX inhibits progression of glioma in vitro and in vivo by increasing miR-378e and decreasing RPN2, providing a novel mechanism for understanding the pathogenesis of glioma.

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Section: Discussionmentioning

confidence: 95%

CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Ding

You

et al. 2019

J Exp Clin Cancer Res

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“…RPN2, TUSC, as well as DDOST are the subunits of OST. The expression of RPN2 is positively correlated with the progression of breast cancers ( Ono et al, 2015 ), non-small cell lung ( Fujita et al, 2015 ), gastric ( Fujimoto et al, 2018 ), esophageal ( Li et al, 2019 ), and colorectal cancers ( Bi and Jiang, 2018 ), whereas TUSC3 was reported as a candidate tumor suppressor ( Vašíčková et al, 2018 ). DDOST, which also acted as advanced glycation end product-receptor 1 ( Li et al, 1996 ), had been reported in regulating AGE, which increased oxidative stress and inflammation and may be involved in liver injury and subsequent carcinogenesis ( Moy et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Prognostic Biomarker DDOST and Its Correlation With Immune Infiltrates in Hepatocellular Carcinoma

et al. 2022

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Background: Dolichyl-diphosphooligosaccharide–protein glycosyltransferase non-catalytic subunit (DDOST) is an important enzyme in the process of high-mannose oligosaccharide transferring in cells. Increasing DDOST expression is associated with impairing liver function and the increase of hepatic fibrosis degrees, hence exacerbating the liver injury. However, the relation between DDOST and hepatocellular carcinoma (HCC) has not been revealed yet.Method: In this study, we evaluated the prognostic value of DDOST in HCC based on data from The Cancer Genome Atlas (TCGA) database. The relationship between DDOST expression and clinical-pathologic features was evaluated by logistic regression, the Wilcoxon signed-rank test, and Kruskal–Wallis test. Prognosis-related factors of HCC including DDOST were evaluated by univariate and multivariate Cox regression and the Kaplan–Meier method. DDOST-related key pathways were identified by gene set enrichment analysis (GSEA). The correlations between DDOST and cancer immune infiltrates were investigated by the single-sample gene set enrichment analysis (ssGSEA) of TCGA data.Results: High DDOST expression was associated with poorer overall survival and disease-specific survival of HCC patients. GSEA suggested that DDOST is closely correlated with cell cycle and immune response via the PPAR signaling pathway. ssGSEA indicated that DDOST expression was positively correlated with the infiltrating levels of Th2 cells and negatively correlated with the infiltration levels of cytotoxic cells.Conclusion: All those findings indicated that DDOST was correlated with prognosis and immune infiltration in HCC.

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“…27 Besides, Li et al found that esophageal cancer cell proliferation and metastasis could be suppressed by RPN2 knockdown. 28 In HCC, Huang et al confirmed that RPN2 was a vital regulator to promote the malignant progression of HCC. 29 Our study presented that RPN2 was highly expressed in HCC.…”

Section: Discussionmentioning

confidence: 99%

Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network

Fang¹,

Liu²,

Zhou³

et al. 2020

CMAR

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Background Many studies have confirmed that circular RNAs (circRNAs) play a key role in the biological progression of cancers. However, the function of a novel circRNA, circ_0046599, in hepatocellular carcinoma (HCC) progression has not been explored. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure the expression of circ_0046599, microRNA (miR)-1258 and Ribophorin II (RPN2). Subcellular fractionation location assay was used to localize circ_0046599 in HCC cells. The circular characteristic of circ_0046599 was verified using Ribonuclease R (RNase R) digestion assay. Besides, cell counting kit 8 (CCK8) assay, colony formation assay, wound healing assay and transwell assay were used to detect cell proliferation, migration and invasion, respectively. The lactate production and glucose level were determined by Lactate and Glucose Assay Kits. Furthermore, the protein levels of glycolysis, metastasis and proliferation-related marker proteins, as well as RPN2 were tested by Western blot (WB) analysis. Moreover, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to confirm the interactions among circ_0046599, miR-1258 and RPN2. In addition, mice xenograft models were applied to observe the effect of circ_0046599 silencing on HCC tumor growth in vivo. Results Circ_0046599 was highly expressed in HCC tissues and cells, and its knockdown could suppress HCC cell proliferation, migration, invasion and glycolysis process. MiR-1258 could be targeted by circ_0046599, and its inhibitor could invert the suppressing effect of circ_0046599 knockdown on HCC progression. Further, RPN2 was a target of miR-1258. Overexpressed RPN2 could reverse the inhibiting effect of miR-1258 overexpression on HCC progression. Also, knockdown of circ_0046599 could restrain HCC tumor growth in vivo. Conclusion Our results provided new evidence that circ_0046599 could promote the progression of HCC by increasing RPN2 expression via sponging miR-1258.

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Ribophorin II promotes cell proliferation, migration, and invasion in esophageal cancer cells in vitro and in vivo

Cited by 8 publications

References 25 publications

CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Prognostic Biomarker DDOST and Its Correlation With Immune Infiltrates in Hepatocellular Carcinoma

<p>Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network</p>

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