Ribonucleic acid synthesis and transport in animal cells at 27°C

Horisberger, Michel A.; Amos, Harold

doi:10.1042/bj1170347

Cited by 20 publications

(6 citation statements)

References 25 publications

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“…The latter level includes reactions such as RNA-processing, packaging of RNA with proteins, and the final transport of ribonucleoproteins through the nuclear envelope pore complexes into the cytoplasm. This interpretation is consistent with previous findings also found in other cells, i.e., that upon temperature lowering, the transport of RNA from nucleus to cytoplasm becomes more reduced than does RNA-synthesis; consequently, RNA accumulates within the nucleus [5,12,13].…”

Section: Transport and Synthesis Of Total Rnasupporting

confidence: 91%

Effect of temperature on nuclear membranes and nucleo-cytoplasmic RNA-transport inTetrahymena grown at different temperatures

Nägel

Wunderlich

1977

J. Membrain Biol.

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The effect of temperature on the nuclear envelope structure and the transport of total RNA and ribosomal subunits from nucleus to cytoplasm was examined in Tetrahymena cells propagated at two different temperatures. Freeze-etch electron microscopy of cells grown at 23 and 18 degrees C detects the emergence of smooth areas on the fracture faces of the nuclear membranes upon lowering the temperature below approximately 15 and approximately 12 degrees C, respectively. Coincident with these freeze-etch changes, a discontinuous decrease is observed in the nucleocytoplasmic RNA-transport; this is probably not due to a cease in RNA-synthesis. Below the thermotropic discontinuity observed in the transport of total RNA in 18 degrees-cells the nucleocytoplasmic transport of the small and large ribosomal subunits is equally retarded. Recent temperature studies on the endoplasmic reticulum membranes of Tetrahymena suggest that the freeze-etch changes in the nuclear membranes are induced by a thermotropic clustering of the membrane lipids. We conclude that this lipid clustering induces the permanent protein constituents in the nuclear envelope pore complexes to change from a relatively "open" into a relatively "closed" state thus causing the observed decrease in RNA-transport.

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Section: Transport and Synthesis Of Total Rnasupporting

confidence: 91%

Effect of temperature on nuclear membranes and nucleo-cytoplasmic RNA-transport inTetrahymena grown at different temperatures

Nägel

Wunderlich

1977

J. Membrain Biol.

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“…Although appreciable amounts of the 40S RNA precursor are processed to mature rRNA within an hour in the developing embryo of Xenopus laevis (21) and in cells cultured from Xenopus (22,23) when maintained at 370C, the kinetics of processing in an adult poikilotherm maintained at ambient temperatures might, predictably, be slower. For example, the rate of RNA synthesis in cultured chick-retinal cells is decreased by about 70-75% after a transition from 370 to 27°C and little processing of ribosomal-precursor RNA is found (27). Results from the present experiments indicate a processing time of at least 4 hr for label to appear in mature 28S rRNA in intact Bufo marinus.…”

Section: Xf)mentioning

confidence: 49%

Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent period

Vancura¹,

Sharp²,

Malt³

1971

J. Clin. Invest.

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A B S T R A C T Processing of RNA in the toad bladder was analyzed by polyacrylamide-gel electrophoresis to determine whether aldosterone causes any changes in the 1 hr before it potentiates transport of sodium ion. No change was found in the quantity or in the specific activity of bulk RNA labeled with uridine-5-3H. In vivo and in vitro with either uridine-5-'H or with methionine-(methyl)-'H as precursors, processing of RNA was extremely slow. Heterodisperse RNA was obvious after 30 min of continuous labeling, but labeling of the 40S precursor of ribosomal RNA was not apparent for 60 min. Labeling of mature 28S and 18S RNA first became apparent after 8 hr. -S RNA was the principal fastmigrating species labeled at 30 min, and 4S RNA was not heavily labeled until 1 hr. Aldosterone (5 x 10-mole/liter) produced no changes. If care were not taken to inhibit metabolism of native bacteria colonizing the bladder, bacterial RNA of high specific activity predominated. We conclude that RNA metabolism in the toad bladder is extraordinarily slow, that a major acceleration of de novo synthesis in response to physiologic doses of aldosterone was not demonstrable, and that some reports to the contrary may have been influenced by artifacts from bacterial RNA metabolism. Earlier evidence for obligatory alterations in RNA metabolism during the latent period is not strong.

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“…The procedure employed was essentially that described by Horisberger and Amos (11) . Cells were prelabeled with 32p04-3 for 12 h before addition of insulin, serum, or BSS followed 4 h later by a 1-h pulse with [14 C]uridine .…”

Section: Nucleotide Pool Determinationsmentioning

confidence: 99%

Stimulation by Insulin of Rna Synthesis in Chick Fibroblasts

Baseman

Paolini

Amos

1974

The Journal of Cell Biology

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After the addition of insulin to monolayers of chick fibroblasts previously incubated in serum-free medium, the rates of protein and RNA synthesis increase continuously during the first 8-10 h . Little stimulation of DNA synthesis or mitosis results with the addition of insulin alone in contrast to the addition of fresh serum which stimulates both markedly .The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity . All major classes of RNA are stimulated ; processing of preribosomal RNA to 28S and 18S and the association of this mature RNA with ribosomes appear to occur normally . The kinetics of stimulation of 5S RNA differ from those of the synthesis of 4S and of ribosomal RNA . Insulin and serum appear to affect the synthesis or stability of certain transcripts differentially .

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Ribonucleic acid synthesis and transport in animal cells at 27°C

Cited by 20 publications

References 25 publications

Effect of temperature on nuclear membranes and nucleo-cytoplasmic RNA-transport inTetrahymena grown at different temperatures

Effect of temperature on nuclear membranes and nucleo-cytoplasmic RNA-transport inTetrahymena grown at different temperatures

Kinetics of RNA synthesis in toad bladder epithelium: action of aldosterone during the latent period

Stimulation by Insulin of Rna Synthesis in Chick Fibroblasts

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